* Corresponding Author
Isolasi mikroalga Aurantiochytrium dari Raja Ampat dan potensinya pada industri bahan baku adjuvant vaksin
https://doi.org/10.22146/jrekpros.72045 Suhendra Suhendra(1*), Lia Septianingsih(2), Tifanny Rizka Ariandi(3), Maratul Husna(4), Zen Adi Laksana(5), Dewi Yuniasih(6), Andri Hutari(7)
(1) Program Studi Teknik Kimia, Fakultas Teknologi Industri, Universitas Ahmad Dahlan Kampus IV, Jl. Ringroad Selatan, Tamanan, Banguntapan, Bantul, D.I Yogyakarta 55164
(2) Program Studi Teknik Kimia, Fakultas Teknologi Industri, Universitas Ahmad Dahlan Kampus IV, Jl. Ringroad Selatan, Tamanan, Banguntapan, Bantul, D.I Yogyakarta 55164
(3) Program Studi Teknik Kimia, Fakultas Teknologi Industri, Universitas Ahmad Dahlan Kampus IV, Jl. Ringroad Selatan, Tamanan, Banguntapan, Bantul, D.I Yogyakarta 55164
(4) Chemical Engineering Department, Kocaeli University, Kabaoğlu, Baki Komsuoğlu bulvarı No:515, Umuttepe, 41001 İzmit/Kocaeli, Turkey, 41000
(5) Departemen Teknik Kimia, Universitas Gadjah Mada, Jl. Grafika No.2, Kampus UGM, Yogyakarta, 55281, Indonesia
(6) Fakultas Kedokteran, Universitas Ahmad Dahlan Kampus IV, Jl. Ringroad Selatan, Tamanan, Banguntapan, Bantul, D.I Yogyakarta 55164
(7) Pendidikan Biologi, Fakultas Pendidikan Universitas Muhammadiah Prof. Dr. HAMKA, Jl. Limau II No.2, Kramat Pela, Kec. Kby. Baru, Kota Jakarta Selatan, DKI Jakarta 12130
(*) Corresponding Author
Abstract
Tulisan ini bertujuan memberikan kontribusi akademis potensi mikroalga Aurantiochytrium dari hutan bakau Indonesia. Tulisan ini mempresentasikan gambaran awal penelitian tentang mikroalga Aurantiochytrium mulai dari teknik isolasi mikroalga dari habitatnya, gambaran produksinya, teknik analisa kualitatif squalene hingga analisa potensi ekonomi dan fungsinya untuk bahan baku adjuvant vaksin. Sebanyak 10 sampel daun bakau diambil dari hutan bakau Raja Ampat, Papua Barat. Teknik isolasi menggunakan metode direct plating method. Setelah aplikasi teknik streaking dari koloni mikroalga yang ada pada sampel ke dalam medium agar, dihasilkan 4 isolat murni. Produksi awal biomassa berbahan baku mikroalga Aurantiochytrium ditampikan dengan nutrisi glucosa, yeast extract, peptone dan campuran air laut dan aquadest. Analisa kualitatif produk yang dihasilkan menggunakan Kromatografi Lapis Tipis (KLT) menunjukkan hasil positif adanya sqalene pada biomassa hasil kultivasi isolat mikroalga Aurantiochytrium. Squalene dari hasil fermentasi mikroalga Aurantiochytrium telah banyak dikaji sebagai sumber bahan baku alternatif yang ramah lingkungan dan berkelanjutan (sustainable), Beberapa adjuvant vaksin, termasuk vaksin covid-19 menggunakan bahan baku mikroalga Aurantiochytrium untuk menggantikan adjuvant vaksin yang bersumber dari ikan hiu laut dalam. Di masa depan, potensi produk mikroalga Aurantiochytrium akan semakin dibutuhkan di banyak industri nutrisi kesehatan dan kosmetik. Mengingat relevansinya untuk masa depan industri strategis terkait di Indonesia, seyogyanya perlu riset mendalam yang lebih banyak dari isolat mikroalga Aurantiochytrium dari hutan bakau Indonesia.
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