Properties of Oryctes Baculovirus Isolated in Indonesia

https://doi.org/10.22146/jpti.9318

Jun Kobayashi(1*), Susamto Somowiyarjo(2)

(1) Mie University
(2) Faculty of Agriculture Universitas Gadjah Mada
(*) Corresponding Author

Abstract


An Indonesian isolate of Oryctes baculovirus was purified from infected midguts of the rhinoceros beetle (Oryctes rhinoceros) by centrifugation on a 10–40% (w/v) sucroese gradient. Morphological features of  nucleocapsid including a tail-like projection were very same as those previously reported. Both protein components of purified particles and restriction fragment electrophoresis profiles of viral DNA were similar to those of their isolates of Oryctes baculovirus, although there were some differences.


Keywords


Baculovirus oryctes; electrophoresis

Full Text:

PDF


References

Bedford, G.O. 1980. Biology, ecology and control of palm rhinoceros beetles. Ann. Rev. Entomol. 25:309-339.

Crawford, A.M. and Sheehan. C. 1985. Replication of Oryctes baculovirus in cell culture: Viral morphogenesis, infectivity and protein synthesis. J. Gen ViroL 66:529-539.

Crawford, A.M., Zelany, B. and Aifiler, A.R. 1986. Genotypic variation in geographical isolates of Oryctes baculovirus. J. Gen Virol. 67:949-952.

Huger, A.M. 1966. A virus disease of the Indian rhinoceros beetle, Oryctes rhinoceros (L) caused by a new type of Insect virus, Rhabdionvirus oryctes gen. n., sp. n.J. Invert,. Phatol 8: 38-51.

Huger, A.M. and Krieg, A. 1991. Baculoviridae. Non occluded baculoviruses. In: J.R. Adams and J.R. Bonami (eds.) Atlas of Invertebrate Viruses, CRC Press, Boca Raton, Fl, pp. 287-319.

Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.

Marschall, K.J. 1970 Introduction of a new virus disease of the coconut rhinoceros beetle in Western Samoa. Nature 225:288.

Matthews, RE.F. 1982. Classification and nomenclature of viruses. lntervirology 17: 52-54.

Payne, C.C. 1974. The isolation and characterization of a virus from Oryctes rhinoceros. J. Gen Virol 25:105-1 16.

Payne, CC., Compson, D. and De Looze, S.M. 1977. Properties of the nucleocapsids of a virus isolated from Orycres rhinoceros. Virology 77: 269-280.

Purrini, K. 1989. Baculovirus oryctes release into Oryctes monoceros population in Tanzania, with special reference to the interaction of virus isolates used in our laboratory infection experiments. J. Invert. Phatol. 53:285.

Zelazny, B. 1976. Transmission of a baculovirus in populations of Oryctes rhinoceros. J. Invert. Pathol. 27:221-227.



DOI: https://doi.org/10.22146/jpti.9318

Article Metrics

Abstract views : 1432 | views : 712

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 1995 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics