* Corresponding Author
Phytoplasma Associated with White-backed Planthopper on Rice Plants in Sidrap Regency, South Sulawesi
https://doi.org/10.22146/jpti.84736 Saipul Abbas(1*), Ernawati Djaya(2), Erwin Najamuddin(3), Amelia Sebayang(4), Ayyub Ar Rahman(5), Aminah Aminah(6), Hasbi Hasbi(7), Surianto Sipi(8), Nur Fathurahman Ridwan(9), Rini Ismayanti(10), Elisurya Ibrahim(11)
(1) Faculty Agriculture, Lambung Mangkurat University, Jln. Jend. A. Yani Km. 36 Banjarbaru, South Kalimantan 70714 Indonesia
(2) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(3) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(4) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(5) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(6) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(7) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(8) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(9) Ecology Laboratory, Biology Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Jln. Colombo No. 1 Yogyakarta, 55281 Indonesia
(10) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(11) Research Center for Food Crops, National Research and Innovation Agency (BRIN) Jln. Raya Bogor Km. 46, Cibinong, Bogor, West Java, 16911 Indonesia
(*) Corresponding Author
Abstract
South Sulawesi is one of the largest rice production centers in Indonesia. Several important diseases of rice plants, such as those caused by viruses and phytoplasmas, can be transmitted by insect vectors, especially leafhoppers and stem plant. Symptoms of diseases caused by viruses and phytoplasmas are quite diverse but visually similar and difficult to distinguish. This study aims to analyze the presence of phytoplasma associated with white-backed planthopper which are commonly found in rice plantations. The research method used is by conducting surveys and explorations of insect samples in six villages in Sidrap District. White-back planthoppers found on rice plantations showing symptoms of yellowing and stunted leaves were sampled for further analysis, including total DNA isolation of insects, standard PCR amplification for insect and Nested-PCR for phytoplasma identification, gene sequencing for both amplicons, and nucleotide analysis using BLAST method and Mega X program. The PCR with CO1 primer successfully amplified a 700 bp amplicon from insects, whereas nested-PCR using fP1/rP7 primers followed by m23SR/R16F2n amplified phytoplasma supposedly around 1800 bp and 1250 bp of 16S RNA gene, respectively. The DNA sequencing analysis results indicate that the insect samples were identified as 83% Sogatella vibix species based on homology percentage analysis using BLAST and Mega X Program. As for the phytoplasma, it leans more towards the 16SrI group or Candidatus phytoplasma asteris (Aster yellows phytoplasma) with a homology percentage of 99%.
Keywords
Full Text:
PDFReferences
Abbas, S., Sulandari, S., Hartono, S., & Trisyono, Y.A. (2020). Molecular Detections and Resistance Response of Six Rice Varieties to Tungroviruses from South Sulawesi. Jurnal Perlindungan Tanaman Indonesia, 24(1), 89-97. https://doi.org/10.22146/jpti.47355
Abbas, S., Sulandari, S., Hartono, S., Daud, I. D., & Djaya, E. (2023). Molecular Detection of Five Mixed Rice Viruses by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Pakistan Journal of Phytopathology, 35(1), 25-34. https://doi.org/10.33866/phytopathol.035.01.0793
Ammar, E.D. 1977. Effect of European wheat striate mosaic, acquired by feeding on diseased plants, on the biology of its planthopper vector Javesella pellucida. Annals of Applied Biology 79(2), 195–202. https://doi.org/10.1111/j.1744-7348.1975.tb01533.x
Asche, M., Wilson, M.R. (1990). The Delphacid genus Sogatella and Related Groups: A Revision with Special Reference to Rice-associated species (Homoptera: Fulgoroidea). Systematic Entomology, 15(1), 1-42. https://doi.org/10.1111/j.1365-3113.1990.tb00301.x
Bertaccini, A., Duduk, B., Paltrinieri, S., & Contaldo, N. (2014). Phytoplasmas and Phytoplasma Diseases: A Severe Threat to Agriculture. American Journal of Plant Sciences, 5(12), 1763–1788. https://doi.org/10.4236/ajps.2014.512191
BPS (Badan Pusat Statistik). (2023). Rice Harvest Area, Production, and Productivity by Province (2020-2022). https://www.bps.go.id/indicator/53/1498/1/luas-panen-produksi-dan-produktivitas-padi-menurut-provinsi.html (Accessed 10 February 2023).
Constable, F. E., Joyce, P. A., & Rodoni, B. C. (2007). A Survey of Key Australian Pome Fruit Growing Districts for Exotic and Endemic Pathogens. Australasian Plant Pathology, 36(2), 165-172. https://doi.org/10.1071/AP07003
Deng, S., & Hiruki, C. (1991). Amplification of 16S rRNA Genes from Culturable and Nonculturable Mollicutes. Journal of Microbiological Methods, 14(1), 53-61. https://doi.org/10.1016/0167-7012(91)90007-D
Doyle, J.J., & Doyle, J.L. (1990). Isolation of Plant DNA from Fresh Tissue. Focus, 12, 13-15. https://www.scienceopen.com/document?vid=e1f34a8c-27b4-41d7-a98d-132d7158d2be
Firrao, G., Andersen, M., Bertaccini, A., Boudon, E., Bové, J. M., Daire, X., Davis, R. E., Fletcher, J., Garnier, M., Gibb, K. S., Gundersen-Rindal, D. E., Harrison, N., Hiruki, C., Kirpatrick, B. C., Jones, P., Kuske, C. R., Lee, I. M., Liefting, L., Marcone, C., … Wang, K. (2004). ‘Candidatus Phytoplasma’, a Taxon for the Wall-less, Non-helical Prokaryotes that Colonize Plant Phloem and Insects. International Journal of Systematic and Evolutionary Microbiology, 54(4), 1243–1255. https://doi.org/10.1099/ijs.0.02854-0
Gibb, K. S., Constable, F. E., Moran, J. R., & Padovan, A. C. (1999). Phytoplasmas in Australian Grapevines-detection, Differentiation and Associated Diseases. Vitis, 38(3), 107-114. https://doi.org/10.5073/vitis.1999.38.107-114
Gundersen, D. E., & Lee, I.-M. (1996). Ultrasensitive Detection of Phytoplasmas by Nested-PCR Assays Using Two Universal Primer Pairs. Phytopathologia Mediterranea, 35(3), 144–151. http://www.jstor.org/stable/42685262
Hibino, H. (1986). Rice grassy stunt virus. Tropical Agriculture Research Series No. 19. Trop. Agr. Res. tent. Min. of Agl. Forestry and Fisheries. Japan. https://www.jircas.go.jp/en/publication/tars/19/165
Hibino, H., & Cabauatan, P. Q. (1987). Infectivity Neutralization of Rice Tungro-associated Viruses Acquired by Vector Leafhoppers. Phytopathology, 77(3), 473-476. https://doi.org/10.1094/Phyto-77-473
Hogenhout, S. A., Oshima, K., Ammar, el-D., Kakizawa, S., Kingdom, H. N., & Namba, S. (2008). Phytoplasmas: Bacteria that Manipulate Plants and Insects. Molecular Plant Pathology, 9(4), 403–423. https://doi.org/10.1111/j.1364-3703.2008.00472.x
Huang, W., Reyes-Caldas, P., Mann, M., Seifbarghi, S., Kahn, A., Almeida, R. P., Béven, L., Heck, M., Hogenhout, S. A., & Coaker, G. (2020). Bacterial Vector-Borne Plant Diseases: Unanswered Questions and Future Directions. Molecular Plant, 13(10), 1379-1393. https://doi.org/10.1016/j.molp.2020.08.010
International Rice Research Institute. 2011. Training on Rice Arthropod Biodiversity and Taxonomy in Thailand. International Rice Research Institute. Los Baños, the Philippines.
Jung, H. Y., Sawayanagi, T., Wongkaew, P., Kakizawa, S., Nishigawa, H., Wei, W., Oshima, K., Miyata, S., Ugaki, M., Hibi, T., & Namba, S. (2003). ‘Candidatus Phytoplasma oryzae’, a Novel Phytoplasma Taxon Associated with Rice Yellow Dwarf Disease. International Journal of Systematic and Evolutionary Microbiology, 53(6), 1925–1929. https://doi.org/10.1099/ijs.0.02531-0
Kumari, S., Nagendran, K., Rai, A. B., Singh, B., Rao, G. P., & Bertaccini, A. (2019). Global Status of Phytoplasma Diseases in Vegetable Crops. Frontiers in Microbiology, 10, 441347. https://doi.org/10.3389/fmicb.2019.01349
Lee, I., Michaels, S. D., Masshardt, A. S., & Amasino, R. M. (1994). The Late‐flowering Phenotype of FRIGIDA and Mutations in LUMINIDEPENDENS is Suppressed in the Landsberg erecta Strain of Arabidopsis. The Plant Journal, 6(6), 903-909. https://doi.org/10.1046/j.1365-313X.1994.6060903.x
Li, S., Hao, W., Lu, G., Huang, J., Liu, C., & Zhou, G. (2015). Occurrence and Identification of a New Vector of Rice Orange Leaf Phytoplasma in South China. Plant Disease, 99(11), 1483–1487. https://doi.org/10.1094/PDIS-12-14-1243-RE
Ling, K.-C. (Ed.) Orange Leaf Disease. In Rice Virus Diseases; International Rice Research Institute: Los Banõs, Philippines, 1972; pp. 79–81.
Mariani, R., & de Remes Lenicov, A.M.M (2017). A New Species of Sogatella (Hemiptera: Delphacidae) from Temperate Argentina. Revista Brasileira de Entomologia, 62(1), 77-81. https://doi.org/10.1016/j.rbe.2017.12.001
Nakashima, K. & Murata, N. (1993). Destructive Plant Diseases Caused by Mycoplasma-like Organisms in Asia. Outlook on Agriculture, 22(1), 53–58. https://doi.org/10.1177/003072709302200110
Nakata, M.M., Chung, N.H., & Kobori, Y. (2019). Insecticide Application and its Effect on the Density of Rice Planthoppers, Nilaparvata lugens and Sogatella furcifera, in Paddy Fields in the Red River Delta, Vietnam. J. Pestic. Sci. 44, 129-135. https://doi.org/10.1584/jpestics.D18-080
Nei, M., & Kumar, S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York. https://www.mimuw.edu.pl/~lukaskoz/teaching/sad2/books/Molecular-Evolution-and-Phylogenetics.pdf
Nesmi, A. K. T. (2016). Diversity of Leafhopper Fauna in Rice and Vegetable Ecosystems [Doctoral dissertation], Department of Agricultural Entomology, College of Horticulture, Vellanikkara. http://14.139.185.57:8080/jspui/handle/123456789/6673
Ramaiah, M., Meshram, N.M., & Dey, D. (2023). Integrative Approaches Establish Colour Polymorphism in the Bamboo-Feeding Leafhopper Mukaria splendida Distant (Hemiptera: Cicadellidae) from India. Insects, 14(1), 44. https://doi.org/10.3390/insects14010044
Rosida, N., Senoaji, W., Ibrahim, E., & Kuswinanti, T. (2023). Molecular Detection of Rice tungro bacilliform virus (RTBV) on Weed Host in South Sulawesi. IOP Conference Series: Earth and Environmental Science, 1160(1), 012054. https://doi.org/10.1088/1755-1315/1160/1/012054
Valarmathi, P., Rabindran, R., Relazhahan, R., Suresh, S., & Robin, S. (2013). First Report of Rice Orange Leaf Disease Phytoplasma (16 SrI) in Rice (Oryza sativa) in India. Australasian Plant Disease Notes, 8, 141–143. https://doi.org/10.1007/s13314-013-0117-7
Wilson, M.R., & Claridge, M.F. (1991). Handbook for the Identification of Leafhoppers and Planthoppers of Rice. CAB International for International Institute of Entomology, in association with Natural Resources Institute: London. 142pp.
Wulandari, I., Mutaqin, K.H., Giyanto, & Hidayat S.H. (2022). Identifikasi Fitoplasma pada Wortel (Daucus carota L.) dan Wereng yang Berasosiasi dengan Penyakit Kuning di Bogor dan Bandung, Jawa Barat. Jurnal Fitopatologi Indonesia, 16(4), 157-165. https://doi.org/10.14692/jfi.16.4.157-165
Zadoks, C.J., & Schein, R.D. (1979). Epidemiology and Plant Disease Management. New York: Oxford University Press. 427 p. https://www.cabdirect.org/cabdirect/abstract/19801361394
DOI: https://doi.org/10.22146/jpti.84736
Article Metrics
Abstract views : 1175
| 
views : 517
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics