* Corresponding Author
Double Infection of Onion yellow dwarf virus and Shallot latent virus in Garlic from Several Regions in Indonesia
https://doi.org/10.22146/jpti.62818 Nurenik Nurenik(1), Sedyo Hartono(2*), Sri Sulandari(3), Susamto Somowiyarjo(4), Argawi Kandito(5)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No.1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No.1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No.1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(4) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No.1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(5) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No.1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(*) Corresponding Author
Abstract
Viruses have been a problem on garlic cultivations in various countries. There are several viruses reported infecting garlic. Genera Potyvirus and Carlavirus are the most common viruses found infecting garlic. Mixed infection on garlic is often designated as a “garlic viral complex”. These viruses can be transmitted through imported garlic seeds. Therefore, it is necessary to conduct early detection of garlic seeds to prevent the epidemic of these viruses. This study aimed to detect Onion yellow dwarf virus (OYDV) and Shallot latent virus (SLV) on garlic. Garlic samples were obtained from Enrekang, Magelang, Temanggung, Tawangmangu, and Yogyakarta. Total RNA was extracted from the samples and subsequently used for RT-PCR using two pairs of specific primers SLV-F/SLV-R and OYDV-F/OYDV-R. Primary pair SLV-F/SLV-R in amplicons sized 276 bp, while OYDV-F/OYDV-R in amplicons sized 112 bp. RT-PCR results showed that OYDV was found in all samples tested in this study. Meanwhile, double infections (OYDV and SLV) were found in eight out of ten samples tested. These results indicated that double infections on garlic were common in Indonesia.
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Abraham, A.D., Kidanemariam, D.B., & Holton, T.A. (2019). Molecular Identification, Incidence and Phylogenetic Analysis of Seven Viruses Infecting Garlic in Ethiopia. European Journal of Plant Pathology, 155(1), 181–191. https://doi.org/10.1007/s10658-019-01760-9
Amanda, D., Syaukat, Y., & Firdaus, M. (2016). Estimating the Market Power in Indonesia Garlic Industry. Journal of International Society for Southeast Asian Agricultural Sciences, 22(2), 66–79.
Araújo, E.R., Higashikawa, F.S., & Lima, M.F. (2018). First Report of Onion yellow dwarf virus and Allexivirus Associated with Noble Garlic in Itajai Valley, Santa Catarina State, Brazil. Summa Phytopathologica, 44(2), 195-196. https://doi.org/10.1590/0100-5405/178028
Bereda, M., Paduch-Cichal, E., & Dąbrowska, E. (2017). Occurrence and Phylogenetic Analysis of Allexiviruses Identified on Garlic from China, Spain and Poland Commercially Available on the Polish Retail Market. European Journal of Plant Pathology, 149(1), 227–237. https://doi.org/10.1007/s10658-017-1171-3
Celli, M.G., Perotto, M.C., Buraschi, D., & Conci, V.C. (2016). Biological and Molecular Characterization of Garlic virus D and its Effects on Yields of Garlic. Acta Horticulturae, 1143, 193-200. https://doi.org/10.17660/ActaHortic.2016.1143.28
Da Silva, L.A., Oliveira, A.S., Melo, F.L., Ardisson- Araújo, D.M.P., Resende, F.V., Resende, R.O., & Ribeiro, B.M. (2019). A New Virus Found in Garlic virus complex is a Member of Possible Novel Genus of the Family Betaflexiviridae (order Tymovirales). PeerJ, 7, e6285. https://doi.org/10.7717/peerj.6285
Gibbs, A. & Mackenzie A. (1997). A Primer Pair for Amplifying Part of the Genome of All Potyvirids by RT-PCR. Journal of Virological Methods, 63, 9–16. https://doi.org/10.1016/s01660934(96)02103-9
Harti, H., Hidayat, S.H., Sobir, & Wiyono, S. (2020). Detection of Major Viruses Infecting Shallot and Molecular Characterization of Onion yellow dwarf virus from Several Locations in Indonesia. Biodiversitas, 21(4), 1697-1701. https://doi.org/10.13057/biodiv/d210451
Hu, X.-X., Lei, Y., Wang, P., Tang, L.-F., He, C.-Z., Song, Y., …, Nie, X.-Z. (2015). Development of a Multiplex Reverse Transcription-PCR Assay for Simultaneous Detection of Garlic Viruses. Journal of Integrative Agriculture, 14(5), 900–908. https://doi.org/10.1016/S2095-3119(14)60892-3
Jemal. K., Abraham, A., & Feyissa, T. (2015). The Occurrence and Distribution of Four Viruses on Garlic (Allium sativum L.) in Ethiopia. International Journal of Basic and Applied Sciences, 4(1), 5–11. Retrieved from http://www.crdeepjournal.org/wp-content/uploads/2015/01/Vol41-2-IJBAS.pdf
Jones, S., Baizan-Edge, A., MacFarlane, S., & Torrance, L. (2017). Viral Diagnostics in Plants Using Next Generation Sequencing: Computational Analysis in Practice. Frontiers in Plant Science, 8, 1770. https://doi.org/10.3389/fpls.2017.01770
Kadwati & Hidayat, S.H. (2015). Deteksi Virus Utama Bawang Merah dan Bawang Putih dari Daerah Jawa Barat dan Jawa Tengah. Jurnal Fitopatologi Indonesia, 11(4), 121–127. https://doi.org/10.14692/jfi.11.4.121
Laili, N.U. & Damayanti, T.A. (2019). Deteksi Virus pada Tanaman Mentimun di Jawa Barat. Agrovigor: Jurnal Agroekoteknologi, 12(1), 8–15. https://doi.org/10.21107/agrovigor.v12i1.5089
Leisova-Svobodova, L. & Karlova-Smekalova, K. (2011). Detection of Garlic Viruses Using SYBR Green Real-time Reverse Transcription-Polymerase Chain Reaction. Journal of Phytopathology, 159(5), 429–434. https://doi.org/10.1111/j.1439-0434.2011.01790.x
Majumder, S. & Baranwal, V.K. (2014). Simultaneous Detection of Four Garlic Viruses by Multiplex Reverse Transcription PCR and their Distribution in Indian Garlic Accessions. Journal of Virological Methods, 202, 34–38. https://doi.org/10.1016/j.jviromet.2014.02.019
Majumder, S., Baranwal, V.K., & Joshi, S. (2008). Simultaneous Detection of Onion yellow dwarf virus and Shallot latent virus in Infected Leaves and Cloves of Garlic by Duplex RT-PCR. Journal of Plant Pathology, 90(2), 371–374. Retrieved from https://www.jstor.org/stable/41998518
Marie-Jeanne, V., Loss, R., Peyre, J., Alliot, B., & Signoret., P. (2000). Differentiation of Poaceae Potyviruses by Reverse Transcription-Polymerase Chain Reaction and Restriction Analysis. Journal
of Phytopathology, 148, 141–151. https://doi.org/10.1046/j.1439-0434.2000.00473.x
Mohammed, H.S., Zicca, S., Manglli, A., Mohamed, M.E., El Siddig, M.A.R., El Hussein, A.A., & Tomassoli, L. (2013). Occurrence and Phylogenetic Analysis of Potyviruses, Carlaviruses and Allexiviruses in Garlic in Sudan. Journal of Phytopathology, 161(9), 642–650. https://doi.org/10.1111/jph.12115
Nam, M., Lee, Y.-H., Park, C.Y., Lee, M.-A., Bae, Y.-S., Lim, S., Lee, J.H. Moon, J.S., & Lee, S.-H. (2015). Development of Multiplex RT-PCR for Simultaneous Detection of Garlic Viruses and the Incidence of Garlic Viral Disease in Garlic Genetic Resource. The Plant Pathology Journal, 31(1), 90–96. https://doi.org/10.5423/PPJ.NT.10.2014.0114
Pauzi, Y.S., Lestari, S.M., & Hidayat, S.H. (2018). Variations of Garlic common latent virus and Shallot latent virus Concentration on Shallot and Garlic. IOP Conference Series: Earth and Environmental Science, 197, 012045. https://doi.org/10.1088/1755-1315/197/1/012045
Pramesh, D. & Baranwal, V.K. (2015). Production of Virus-free Garlic (Allium sativum L.) through Meristem Tip Culture after Solar or Hot Air Treatment of Cloves. The Journal of Horticultural Science and Biotechnology, 90(2), 180–186. https://doi.org/10.1080/14620316.2015.11513170
Pramesh, D., Islam, S., Singh, J., & Baranwal, V.K. (2012). Serological and PCR Detection of Garlic common latent virus Associated with Garlic Accessions in India. Medicinal Plants-International Journal of Phytomedicines and Related Industries, 4(1), 17–22. https://doi.org/10.1080/14620316.2015.11513170
Putri, C.A. & Hidayat, S.H. (2020). Sensitivity of Serological and Polymerase Chain Reaction Methods for Detection of Viruses in Allium spp. IOP Conference Series: Earth and Environmental Science, 468, 01203. https://doi.org/10.1088/1755-1315/468/1/012023
Swari, F.S.P., Subandiyah, S., & Hartono, S. (2015). Deteksi dan Identifikasi Virus-virus yang Menginfeksi Bawang Merah di Kabupaten Bantul, Yogyakarta, Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 1(5), 961–968. https://doi.org/10.13057/psnmbi/m010501
Takaichi, M., Nagakubo, T., & Oeda, K. (2001). Mixed Virus Infections of Garlic Determined by a Multivalent Polyclonal Antiserum and Virus Effects on Disease Symptoms. Plant Disease, 85(1), 71–75. https://doi.org/10.1094/PDIS.2001.85.1.71
Wylie, S.J., Li, H., Saqib, M., & Jones, M.G.K. (2014). The Global Trade in Fresh Produce and the Vagility of Plant Viruses: A Case Study in Garlic. PLoS ONE, 9(8), e105044. https://doi.org/10.1371/journal.pone.0105044
DOI: https://doi.org/10.22146/jpti.62818
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