Pemanfaatan Antibodi Monoklonal dalam I-ELISA untuk Deteksi Penyebab Penyakit Busuk Pucuk Kelapa (Phytophthora palmivora)

https://doi.org/10.22146/jpti.12762

Susamto Somowiyarjo(1*), Daisy Prapto Sriyanti(2), Mulyadi Mulyadi(3), Suryanti Suryanti(4), Y. M.S. Maryudani(5), Bambang Hadisutrisno(6)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pettanian UPN Veteran Yogyakarta
(3) Fakultas Pertanian Universitas Gadjah Mada
(4) Fakultas Pertanian Universitas Gadjah Mada
(5) Fakultas Pertanian Universitas Gadjah Mada
(6) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Mice monoclonal antibodies (MCAs) against Phytophthora palmivora, Butl. (the pathogen of coconut bud rot) have been produced and tested. The selected hybridoma (Ab-PM.3) secreted monoclonal antibodies of IgM subclass and the titer in vitro of 10^2 - 1O^3, was employed to develop Indirect-Enzyme Linked lmmunosorbent Assay. This assay was able to differenciate coconut isolate of P. palmivora not only from cocoa and pepper isolate of the same species, but also from other fungus isolated from coconut bud rot diseased coconut (Fusarium sp., Colletotrichum sp., and Thielaviopsis sp.). This assay was able to differenciate P. pulmivora isolate from diseased coconut with those isolated from cocoa and pepper, and other fungus isolated from diseased coconut, thereby providing specific diagnostic tool for coconut bud rot which is needed for supporting the application of the concept of integrated pest mangement on coconut.

Keywords


monoclonal antibodies; Phytophthora palmivora; I-ELISA

Full Text:

PDF


References

Anonim, 1992. Survai Penyakit Busuk Pucuk (Phytophthora sp.) pada Beberapa Varietas Kelapa di Indonesia. Direktorat Jenderal Perkebunan Departemen Pertanian. 35 p.

Burrell, R.G., Clayton, C.W., Gallegly, M.E. and Lilly, V.G. 1966. Factors Affecting the Antigenicity of the Mycelium of Three Species of Phytopthora. Phytopathology 56: 422-426.

Cahill, D.M. & Hardham, A.R. 1984. Exploitation of zoospore Taxis in the Development of a Novel Dipstick Immunoassay for the Specific Detection of Phytophthora cinnamomi. Phytopathology 84: 193-200.

Clark, M.F. & A.N. Adam. 1977. Characteristics of the Microplate Method of Enzyme-Linked Imrnunosorbent Assay for the Detection of Plant Viruses. J. Gen. Virol. 34: 475-483.

Dietzgen, R.G. & E. Sander. 1982. Monoclonal Antibodies Against Plant Virus. Arch. Virol. 74: 197-204.

Galfre, G., Howe, S.C., Milstein, C., butcher, G.W. & Howard, J.C. 1977. Antibodies to Major Histocompatibility antigens Produced by Hibrid cell Lines. Nature 266: 550-552.

Girsang, P. 1990. Situasi Perkelapaan di Indonesia. Manggar 3 (3): 40-46.

Koenig, R., 1981. Indirect-ELISA Methods for the Broad Specificity Detection of Plant Viruses. J. Gen. Virol. 67. 53-62.

Kohler, G. & Milstein, C. 1975. Continuous Culture of Fused Cells Screening Antibodies of Pre-defined Specificity. Nature 256: 495-497.

Luben, R.A., P. Brazeau, P. Bohlen, & R. Guillemin. 1982. Monoclonal antibodies to Hypothalamicgrowth Hormone-Releasing Factor with Picomoles of Antigen. Science 218:887-889.

Modjo, H.S., Hadisutrisno, B., & Sri Sumarni, V. 1989. Kajian Tipe Pasangan (Mating Type) Empat Isolat Phytophthora palmivora. Kongres Nasional PFI X dan Seminar Ilmiah,Denpasar, Bali, 5 p.

Schreier, M., G. Kohler, H. Hengartner, C. Berek, M. Trucco, & L. Forni. 1980. Hybridoma Techniques. Cold Spring Harbor Laboratory, New York, 65 p.

Sikora, K & H.M. Smedley. 1984. Monoclonal Antibodies. Blackwell Scientific Publications, London, 132 p.

Somowiyarjo, S., N. Sako, & F. Nonaka. 1985. Application of Precoated Indirect-Enzyme Linked Immunosorbent Assay for Detecting Zucchini Yellow Mosaic Virus. Ann. Phytopath. Soc. Japan 51: 569-575.

Spitz, M., L. Spitz, R. Thorpe & E. Eugui. 1984. Intrasplenic Primary Imrmunization for the Production Monoclonal Antibodies. J. Immunol. Methods 70: 39-43.

Thomas, J.E., P.R. Massalski & B.D. Hansson, 1986. Production of Monoclonal Antibodies to African Cassava Mosaic Virus and Differences in their Reactivities with Other White Fly-Transmitted Gemini Viruses. J. Gen. Virol. 67: 2739-2748.



DOI: https://doi.org/10.22146/jpti.12762

Article Metrics

Abstract views : 1905 | views : 4021

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 1999 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics