Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus

https://doi.org/10.22146/jpti.12103

Sholeh Avivi(1*), Sudarsono Sudarsono(2), Satriyas Ilyas(3), Hajrial Aswidinnoor(4)

(1) Fakultas Pertanian Universitas Jember
(2) Fakultas Pertanian Institut Pertanian Bogor
(3) Fakultas Pertanian Institut Pertanian Bogor
(4) Fakultas Pertanian Institut Pertanian Bogor
(*) Corresponding Author

Abstract


The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamiana
T0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.

Keywords


N. benthamiana; cp-PStV; resistance

Full Text:

PDF


References

Al-Kaff, N.S., S.N. Covey, M.M. Kreike, A.M. Page, R. Pinder, & P.J. Dale, 1998. Transcriptional and Post-Transcriptional Plant Gene Silencing in Response to a Pathogen. Science, 279:2113-2115.

Anandalakshmi, R., G.J. Pruss, X. Ge, R. Marathe, T.H. Smith, & V.B. Vance. 1998. A Viral Suppressor of Gene Silencing in Plant. Proc. Natl. Acad. Sci. USA. 95:13079-13084.

Ange, S.M. & D.C. Baulcombe. 1997. Consistent Gene Silencing in Transgenic Plants Expressing a Replicating Potato Virus X RNA. EMBO J. 16:3675-3684.

Atkinson, R.G, L.R.F. Bieleski, A.P. Gleave, DJ, Jannsen, & B.A.M. Morris. 1998. Post-Transcriptional Silencing of Chalcone Synthase in Petunia Using a Gemini virus-Based Episoma Vector. Plant J. 15:593-604.

Barker, .H., B. Reavy, D. Kara, McGeachy, & S. Dawson. 1998. Transformation of Nicotiana bethamiana With the Potato Mop-Top Virus Coat Protein. MPMI. 11(7):626-633.

Beachy, R.N. 1990. Coat Protein Mediated Resistance in Transgenic Plants. p 13-22, In T.P. Pirone & J.G. Shaw (eds). Viral Genes and Plant Pathogenesis. Springer-Verlag. New York. 215p.

Brigneti, G., 0. Voinnet, L. Wan-Xiang, J. Liang-Hui, S.W. Ding, & D.C. Baulcombe. 1998. Viral Pathogenicity Determinant are Suppressors of Transgene Silencing in Nicotiana bethamiana. EMBO J. 17:6739- 6746.

Dietzgen, R.G., Z. Xu, & P.Y. Teycheney. 1994. Digoxigenin-Labeled c-RNA Probes for the Detection of Two Potyviruses Infecting Peanut (Arachis hypogaea L.). Plant Disease 78(7):708- 711.

Frank G.F., A. Stuart, Macfarlane, & D.C. Baulcombe. 1999. Gene Silencing Without DNA: RNA-Mediated Cross-Protection between Viruses. The Plant Cell. 11: 1207-1215.

Gonsalves, D. & J.L. Slightom. 1993. Coat Protein-Mediated Protection: Analyses of Transgenic Plants for Resistance in a Variety of Crops. Sem. Virology. 4:397-406.

Grumet, R. 1990. Genetically Engineered Plant Virus Resistance. Hort. Science. 25(5):508-513.

Hammond, J. 1998. Resistance to Plant Viruses-an Overview. p 163-171. In A. Hadidi, R.K. Khetarpal, & H. Koganezawa (eds.) Plant Virus Disease Control. APS Press. The American Phytopathological Society. St. Paul, Minnesota.

Hull, R. 1990. Non-Conventional Resistance to Viruses in Plants Concepts and Risk. P289-303. In J.P. Gustafson (ed.). Gene Manipulation in Plant Improvement II. Penum Press, New York.

Kaniewski, W. & C. Lawson. 1998. Coat Protein and Replicase-Mediated Resistance to Plant Viruses. In A. Hadidi, R.K. Khetarpal, & H. Koganezawa (eds.) Plant Virus Disease Control. APS Press. The American Phytopathological Society. St. Paul, Minnesota.

Mat Akin, H. 1998. Peanut stripe virus Strain Indonesia: Variasi Biologi, Deteksi Molekuler, Pengklonan, dan Determinasi Urutan Nukleotida 3' Genom RNA PStV, serta Analisis Keragaman dan Filogenetika Berdasarkan Gen cp dan 3' utr. Disertasi. Program Pascasarjana. IPB. Bogor.

Middleton, K.J. & N. Saleh. 1988. Peanut Stripe Disease in Indonesia and the Aciar Project. Summary Proceedings of the First Coordinators' Meeting on Peanut Stripe Virus. ICRISAT, India:4-6.

Natural, M.P., F.L. Mangaban, & L.D. Valencia. 1989. Groundnut Research in the Philippines. Second Coordinators Meeting on Peanut Stripe Virus (PStV). Agrikam. 5(2):71-83.

Newton, T.R. 1997. Agrobacterium Mediated Transformation of Peanut. Honours Thesis. The University of Queensland.

Saleh, N. & Y. Baliadi. 1992. Penyakit Virus Bilur Kacang Tanali (Peanut stripe virus) dan Usaha Pengendaliannya. Balai Penelitian Tanaman Pangan. Malang. 22p.

Sudarsono, 1993. Transgenic Burley and Flue-Cured Tobacco with Resistance to Various Isolates of Potato Virus Y (PVY). NC State University, Raleigh, NC, USA.

Sudarsono, S. Tumbelaka, & S. Ilyas. 1997. Penurunan Hasil Akibat Peanut stripe virus dan Penularan Virus Lewat Benih pada Kacang Tanah. Hayati: 55-58.

Thomson, D. & R.G Dietzgen. 1995. Detection of DNA and RNA Plant Viruses by PCR and RT-PCR Using a Rapid Virus Release Protocol without Tissue Homogenization. J. Virological Methods 54:85-95.

Xu, Z., C. Kunrong, Z. Zongyi, C. Jinxiang, & K.J. Middleton. 1990. Research on Peanut Stripe Virus in China. 6p. (Unpublished).

Xu, Z., Z. Zhang, K. Chen, J. Chen, & D.V.R. Reddy. 1995. Current Research on Groundnut Virus Diseases in China. In the Proceedings of the Groundnut Virus Disesases in Asia-Pacific Region working Group Meeting in Thailand in March. Thailand.



DOI: https://doi.org/10.22146/jpti.12103

Article Metrics

Abstract views : 1655 | views : 834

Refbacks

  • There are currently no refbacks.




Copyright (c) 2005 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics