Isolasi dan Karakterisasi Kitinase Akar Tusam (Pinus merkusii Jungh. et de Vriese) Roots yang Bersimbiosis dengan Fungi Ektomikoriza

https://doi.org/10.22146/jpti.12099

A. Handayani(1*), S. M. Wisyastuti(2), S. Margino(3)

(1) Pusat Studi Bioteknologi Universitas Gadjah Mada
(2) Fakultas Kehutanan Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The experiment was aimed to detect the activity and characterize the chitinase of tusam root during ectomycorrhizal symbiosis. Tusam inoculated with tusam stands soil from Kaliurang as fungi inocula. Crude proteins were isolated from 4, 6, and 8 weeks age of tusam. Chitinase activity and isoform were detected using glycol chitin as a subtrat. The enzyme was purified by ammonium sulfat precipitation, dialysis, followed by gel filtration chromatography. The results showed that tusam prodced chitinasse with a molecular weight of approximately 52kDa. The optimum activity was at pH 5 and temperature of 30°C.

Keywords


isolation; characterization; chitinase; ectomycorrhiza; tusam

Full Text:

PDF


References

Albrecth, C., A. Asselin., Y. Piche & F. Lapeyrie. 1994. Chitinase Activities are Induced in Eucalyptus globulus Roots by Ectomycorrhizal or Pathogenic Fungi During Early Colonization. Physiol. Plantarum. 91: 104-110.

Anggoro, M.D. 2003. Isolasi dan Karakterisasi β-1,3 Glukanase Akar Tusam (Pinus merkusii Jungh. et de Vriese) yang Berasosiasi dengan Jamur Pembentuk Ektomikorisa. Tesis. Pusat Studi Bioteknologi, UGM, Yogyakarta. Tidak Dipublikasikan.

Brundertt, M., N. Bougher., B. Dell., T Grove & N. Malajczuk. 1996. Working with Mycorrhizas in Forestry and Agriculture. ACIAR, Canberra. 374p.

Cabib, E. 1987. The Synthesis and Degradation of Chitin. dalam: A. Meister (Ed). Advances in Enzymology and Related Areas of Molecular Biology. John Wiley and Sons, New York. 59: 59-101.

Duschene, L. C. 1996. Role of Mycorrhizae Fungi in Biocontrol dalam. Pfleger, F.L. dan R.G. Linderman. Mycorrhizae and Plant Health. APS Press. St. Paul, Minesota. 334p.

Hartman, G.E., C.K. Hayes., M. Lorito., R.M. Broadway., A. Diepitro., C. Peterbeauer & A. Tronsmo. 1993. Chitynolitic Enzymes of Trichoderma harzianum: Purification Chitobiosidase and Endochitinase. Phytopathol. 83: 313-318.

Hou, W.C., Y.C. Chen & Y. H. Lin. 1998. Chitinase Activity of Sweet Potato (Ipomoea batatas [L] Lam var Tainong 57). Bot. Bull. Acad. Sin. 39: 93-97.

Hunyh, N.V. 2002. Volarization of Chitin with the Aim of Production of Biofungicides. VAR Veterinary and Agrochemical Research Centre.

Laemmli, U.K. 1970. Cleaveage of Sturtural Protein During The Assembly of The Head of Bacteriophage T4. Nature, London. 227: 680-685.

Lin, Z.F., D. Wu., A. Luo & W. Zhang. 1982. Chitinase from Seed of Zea mays and Coix Lachrymajobi L. Purification and some Properties. Process Biochem. 27: 83-88.

Muzarelli, R.A.A. & M.G. Peter. 1997. Gel Electrophoretic analysisi of Chitinase, Chitonase and Chitin Deacetylase. In Chitin Handbook. European Chitin Society. Torette, AN, Italy. 237-242.

Pozo, M.J., C.A. Aguilar, E. D. Gaudot & J. M. Barea. 1998. Chitonase and Chitinase Activities in Tomato Roots During Interactions with Arbuscular Mycorrhizal Fungi or Phytophthora parasitica. Experimen. Botan. 49 (327): 1729-1739.

Salzer, P., B. Hubner, A. Sirrenberg & A. Hager. 1997. Differential Effect of Purified Spruce Chitinase and β-1,3 Glukanases on the Activity of Ellicitors from Ectomycorrhizal Fungi. Plant. Physiol. 114: 957-968.

Salzer, P., H. Corbiere, M. Engelhard, R. Godat, T. Boller & A. Wiemkema. 2002. Molecular Biology of Symbiosis. Molecular Biology of Symbiosis. Htm. 1-5.

Sauter, M. & A. Hager. 1989. The Mycorrhizal Fungus Amanita muscaria Induces Chitinase Activity in Roots Suspension-Cultured Cells of Its Host Picea abies. Planta. 179: 61-66.

Soerianegara, I. & M. J. Lemmens. 1996. PROSEA. Plant Resources of South - East Asia 5 : (1) Timber Trees; Major Commercial Timbers. Prosea Foundation, Bogor. 349-357.

Sumardi & S. M. Widyastuti. 2000. Identifikasi Gangguan pada Persemaian Pinus Penanggulangan serta Pencegahannya. Laporan penelitian Kerjasama Fakultas Kehutanan UGM-Perhutani.

Trudel, J. & A. Asselin. 1989. Detection of Chitinase Activity after Polyacriylamide Gel Electrophoresis. Anal. Biochem. 178: 362-366.

Vannini, A., C. Coruso, L. Leonardi, E. Rugini, E. Chiarot, C. Caporale & V. Buonocore. 1999. Antifungal Properties of Chitinase from Castenea sativa against Hypovirulent and Virulent Strains of the Chesnut Blight Fungus Crryponectria parasitica. Physiol. Mol. Plant. Pathol. 55: 29-35.

Yamagami, T. & G. Funatsu. 1993. Purification and some Properties of the Three Chitinases from the Seed of Rye. Biosic. Biotech. Biochem. 57: 643-647.



DOI: https://doi.org/10.22146/jpti.12099

Article Metrics

Abstract views : 1229 | views : 935

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2005 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics