Kemampuan Pseudomonas putida Pf-20 dan 24.7B untuk Memperbaiki Sifat Kimia Media Tumbuh dan Ketahanan Terinduksi Tembakau H877 terhadap Cucumber mosaic virus

https://doi.org/10.22146/jpti.12085

W. S. Wahyuni(1*), A. Iwan(2), A. Mudjiharjati(3), T. C. Setyowati(4), H. Purwiko(5)

(1) Jurusan HPT Fakultas Pertanian Universitas Gadjah Mada
(2) Jurusan HPT Fakultas Pertanian Universitas Negeri Jember
(3) Jurusan Tanah Fakultas Pertanian Universitas Negeri Jember
(4) Jurusan Tanah Fakultas Pertanian Universitas Negeri Jember
(5) Jurusan Tanah Fakultas Pertanian Universitas Negeri Jember
(*) Corresponding Author

Abstract


P. putida Pf-20 from medium M1 (paddy soil, leaves compost and manure compost, 1:3:2) has a higher affinity and more rapid to solubilize phosphate on Pikovskaya medium, rather than P. putida 27.4B. Those affinities of both strains from the media M1 with CMV-48 were much greater than that of medium without CMV. After 64 days planted, the content of N total, C-organic, CEC, K20, and P205 were better on medium M1, but the pH was little bit decreased. The reduction of P04 and Fe in medium M1 could due to the bacterial growth, and for producing siderophore, particularly on the media with viral treatment. These bacteria strains did not affect on the plant height and number of leaves on media M1, M2 or M3, however, these bacteria affected on the total root length and root densities in the medium M1 with virus. The more available nutrient in the medium, the more rapid bacteria colonized rhizosphere and roots. Both of these bacteria were effective to reduce the disease severity of CMV on tobacco H877. The medium M1 was the best medium for bacterial and tobacco growth.


Keywords


bacteria strains; disease severity of CMV

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References

Addy, H.S. 2003. Pengaruh introduksi cacing merah (Lumbricus rubellus) dan Pseudomonas putida Pf-20 pada beberapa medium tumbuh terhadap keparahan penyakit CMV pada tanaman ketimun. Skripsi. Fak. Pertanian UNEJ. Jember.

Andayani, S.D. 2004. lnteraksi P. putida Pf-20 dengan CMV dan TMV pada tembakau H877. Skripsi. Fak. Pertanian UNEJ. Jember.

Brimecombe, M.J., P.A. de Lej, & J.M. Lynch. 2001. The effect of root exudates on rhizosphere microbial populations. pp. 95-140. In R. Pinton, Z. Varanini & P. Nannipieri (eds.). The Rhizosphere. Marcell Dekker, Inc. New York.

Crowley, D. 2001. Function of siderophore in the plant Rhizosphere. pp. 223-261. In R. Pinton, Z. Varanini & P. Nannipieri (eds.). The Rhizosphere. Marcell Dekker, Inc. New York.

Firky-Ali. 2001. Uji selektivitas bakteri pelarut fosfat pada media cair molase dengan beberapa konsentrasi. Skripsi. Fakultas Pertanian UNEJ.

Leeman M., J.A. van Pelt, M.J. Hendrickx, R.J. Scheffer, P.H.A.M. Baker, & B.Schippers. 1995. Biocontrol of Fusarium wilt of radish in commercial greenhouse trials by seed treatment with Pseudomonas fluorescens WCS374. Phytopatliology 85: 1301-305.

Leeman, M., F.M. den Ouden, J.A. van Pelt, F.P.M. Dirkx, H. Steijl, P.H.A.M. Bakker, & B. Schippers. 1996. Iron avaibility affects induction of systemic resistance to Fusarium wilt of radish by Pseudomonas fluorescens. Phytopathology 86: 149-155.

Neumann, G. & V. Romheld. 2001. The release of root exudates as affected by the plant's physiological status. pp. 41-93. In R. Pinton, Z. Varanini & P. Nannipieri (eds.). The Rhizosphere, Marcell Dekker, Inc. New York.

Ongena, M., F. Daayl, P. Jacques, P. Thonart, N. Benhamou, T.C. Paulitz, P. Cornells, N. Koedam & R.R. Belanger. 1999. Protection cucumber againts Pythium root rot by fluorescent pseudomonads: predominant role of induced resistance over siderophores and antibiosis. Plant Pathol. 48: 66-76.

Ongena, M., F. Daayl, P. Jacques, P. Thonart, B. Benhamou, T.C. Paulitz & R.R. Belanger. 2000. Systemic induction of phytoalexins in cucumber in response to treatments with fluorescent pseudomonads. Plant Pathol.49: 523-530.

Paramita, D.A. 2004. Pemanfaatan cacing merah (Lumbricus rubellus) untuk meningkatkan peran Pseudomonas putida Pf- 20 dan 27.4B dalam menginduksi ketahanan sistemik ketimun cv. Verona F-1 terhadap CMV. Skripsi SJ. Fak. Pertanian UNEJ. Jember.

Paul, E.A. & F.E. Clark. 1989. Soil Microbiology and Biochemistry. Academic Press. New York-London.

Premono, E.M., A.M. Moaward & P.L.G. Vlek. 1996. Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and survival in the rhizosphere. Ind. J. Crop Scie. 11: 13-23.

Varanini, Z. & R. Pinton. 2001. Direct versus indirect effect of soil humic substances on plant growth and nutrition. pp. 141-157. In R. Pinton, Z. Varanini & P. Nannipieri (eds.). The Rhizosphere. Marcell Dekker, Inc. New York.

Wahyuni, W.S., R. Yutriono & S. Winarso. 2003. Pengaruh konsentrasi besi dalam media tanam pada aktivitas Pseudomonas putida Pf-20 untuk menginduksi ketahanan tembakau terhadap Cucumber mosaic virus. Hayati 10:130-133.

Wahyuni, W.S. 2003. Kemampuan Pseudomonas putida Pf-20 untuk mengendalikan Cucumber mosaic virus. Hlm 111-115. Pros. Kongres XVII & Seminar PFI. Bandung, 6-8 Agustus 2003.

Wahyuni, W.S., H.S. Addy, B. Arman, & T.C. Setyowati. 2006. Sinergisme Lumbricus rubellus dengan Pseudomonas putida Pf-20 dalam menginduksi ketahanan sistemik mentimun terhadap Cucumber mosaic virus. Hayati. Siap terbit.



DOI: https://doi.org/10.22146/jpti.12085

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