OPTIMATION OF COMPOSTION OF SPAN 80-CRODURET 50-PROPYLENE GLYCOL AS EMULSIFIER OF STRAWBERRY JUICE (Fragaria vesca L.) EMULGEL AND TRANSPORT TEST THROUGH SHED SNAKE SKIN MEMBRANE

https://doi.org/10.14499/jfps

DIAN EKA ERMAWATI(1*)

(1) DEPARTEMEN OF PHARMACEUTICS, FACULTY OF PHARMACY, GADJAH MADA UNIVERSITY, YOGYAKARTA
(*) Corresponding Author

Abstract


Strawberry (Fragaria vesca L.) contains many nutrients that are beneficial to health because rich of antioxidant such as ascorbic acid, quercetin-3-β-D–glucoside, anthocyanin pelargonidin-3-O-glucoside and cyanidin-3-glucoside. Amount of anthocyanins and flavonoids in strawberry during process will decline respectively 53% for pelargonidin-3-O-glucoside and 40% for flavonoid quercetin. Using water-oil emulsion (w/o) topically less convenient, so it needs to be dispersed in the matrix gel to help sustain the permeation of the active components of strawberry juice into the stratum corneum. Emulsion consisting of water and oil components are not mixed with each other, thus requiring additional emulsifier to create a system that is homogeneous and stable.

The proportion of combination of emulsifier as span 80, croduret 50, propylene glycol are optimized, to produce an optimum formula with good physical stability. The optimization method was Simplex Lattice Design by using Design Expert® software 7.1.5. The results of the response of the assessed physical parameter were viscosity, separation ratio (F), and diameter of emulsion globule. The optimum formula have measured physical stability test, active ingredient of flavonoid and anthocyanins, which  then was conducted  transport  test  through the membrane of shed snake skin by Franz’s model diffusion cells with the flavonoid concentration parameters that were able to pass through the membrane.

Emulsion w/o that use combination of 2,00% Span 80; 4,00% croduret 50, and 2,00% of propylene glycol which produce a physically stable emulsion base on Simplex Lattice Design [p-value> 0.05]. Strawberry juice contains 23,3 % of flavonoid, 13,23% of anthocyanin, and IC50 331 µg/mL. The optimum formula of emulgel contains 20% of flavonoids and 11.67% of anthocyanin. The cumulative amount of flavonoid which was transported through the shed snake skin membrane was 117,14 µg/cm2  during five hours. The permeability of membrane shed snake skins is 2,84x10-5 µg/cm2and flux value is 6,6x10-5 µg/secon.

 

Keywords : strawberry juice, emulsifier, Simplex Lattice Design, emulgel, flavonoid




References

Ali, M.D., Alam, M.I., Shamim, M., Imam, F., Anwer, T., Siddiqui, M.R., dkk., 2012. Design and Characterization of Nanostructure Topical Gel of Betamethasone Dipropionate for Psoriasis.

Bolton, S. dan Bon, C., 2003. Pharmaceutical Statistics: Practical and Clinical Applications, Revised and Expanded, 4 edition. ed. CRC Press, New York.

Kubo, H., Fujii, K., Kawabe, T., Matsumoto, S., Kishida, H., dan Hosoe, K., 2008. Food content of ubiquinol-10 and ubiquinone-10 in the Japanese diet. Journal of Food Composition and Analysis, 21: 199–210.

Korać, R., Krajišnik, D., Savić, S., Pantelić, I., Jovančić, P., Cekić, N., dkk., 2014. A new class of emulsion systems – Fast inverted o/w emulsions: Formulation approach, physical stability and colloidal structure. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 461: 267–278.

Lee, J., Durst, W.R., Wrolstad, E.R. Determination of Total Monomeric Anthocyanin Pigment Content of Fruits Juices, Beverages, Natural Colorants, and Wines by pH Differential Methds : Collaborative Study. Journal of AOAC International Vol.88, No.5, 2005.

Musa, K.H., Abdullah, A., Kuswandi, B., dan Hidayat, M.A., 2013. A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity. Food Chemistry, 141: 4102–4106.

Patras, A., Brunton, N.P., Tiwari, B.K., dan Butler, F., 2009. Stability and Degradation Kinetics of Bioactive Compounds and Colour in Strawberry Jam during Storage. Food and Bioprocess Technology, 4: 1245–1252.

Patel D.M. and Patel N.M. Gastroretentive drug delivery system of carbamazepine: Formulation optimization using simplex lattice design : A technical note, AAPS PharmSciTech. 2007; 8(1): 82–86.

Prajapati S. D. and Patel D.L. Floating matrix tablets of domperidone : formulation and optimization using simplex lattice design. Thai J. Pharm. Sci. 2009; 33:113-122.

Sapei, L. dan Hwa, L., 2014. Study on the Kinetics of Vitamin C Degradation in Fresh Strawberry Juices. Procedia Chemistry, International Conference and Workshop on Chemical Engineering UNPAR 2013 (ICCE UNPAR 2013) 9: 62–68.

Satish, K.M., Saugat, A., Ameya, A.D. Apllication of Simplex Lattice Design in Formulation and Development of Buoyant Matrices of Dipyridamole. Journal : Journal of Applied Pharmaceutical Science Vol.2[12] pp.107-111. Desember 2012. Doi : 10.7324/JAPS.2012.21221.

Suhesti, T.S., Fudholi, A., Martien, R., Apllication of Simplex Lattice Design for The Optimization of The Pyroxicam Nanosuspensions Formulation using Evaporative Antisolvent Technique. Journal : International Journal of Pharmaceutical and Clinical research. 2016. 8[5] suppl : 433-439. ICPAPS 2015. Faculty of Pharmacy. Gadjah Mada University. Yogyakarta.

Vollrath, K.M., Subongkot, T., Ngawhirunpat, T. Model Membrane From Shed Snake Skins. World Academy of Science Enginereing and Technology. International Journal of Medical Health, Biomedical and Pharmaceutical Engineering Vol : 7, No : 10, 2013.

Wang, S.Y. dan Jiao, H., 2000. Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. Journal of Agricultural and Food Chemistry, 48: 5677–5684.

Zhu, Q., Nakagawa, T., Kishikawa, A., Ohnuki, K., dan Shimizu, K., 2015. In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria × ananassa var. Amaou). Journal of Functional Foods, 13: 38–49.



DOI: https://doi.org/10.14499/jfps

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