The Consumption Effect of Indigenous Probiotic Powder Lactobacillus plantarum Dad-13 on Gut Microbiota Population and Short Chain Fatty Acids in Students of SMPN 1 Pangururan, Samosir

https://doi.org/10.22146/ifnp.86598

Nancy Eka Putri Manurung(1), Pratama Nur Hasan(2), Mohammad Juffrie(3), Tyas Utami(4), Rini Yanti(5), Endang Sutriswati Rahayu(6*)

(1) Faculty of Agricultural Technology, Gadjah Mada University
(2) Faculty of Agricultural Technology, Universitas Gadjah Mada
(3) Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
(4) Faculty of Agricultural Technology, Universitas Gadjah Mada
(5) Faculty of Agricultural Technology, Universitas Gadjah Mada
(6) Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The human intestine is a diverse ecosystem populated by microbiota affected by several factors, including age. The aim of this research was to evaluate the effects of the probiotic powder Lactobacillus plantarum Dad-13 on the numbers of gut microbiota, short-chain fatty acids (SCFA), and fecal characteristics in healthy adolescents. This research was conducted at SMPN 1 Pangururan, Samosir, with a randomized, double-blind, parallel placebo-controlled trial. 54 healthy adolescents aged 13 to 14 were divided into two groups, one consumed a gram of skimmed milk powder (placebo group) and the other ingested powder containing L. plantarum Dad-13 with 1.18 × 109 CFU/gram (probiotic group). After 33 days of intervention, the height of placebo group (149.42 ± 5.03 cm) and probiotic group (154.37 ± 4.67 cm) increased significantly. Significant increases in body weight (44.35 kg ± 4.61 to 45.20 kg ± 4.78) and BMI (and 18.77 ± 2.12 to 18.99 ± 2.11) were observed in the probiotic group. In the probiotic group, the numbers of gut microbiota were not significantly affected (p > 0.05). The amount of SCFA and fecal characteristics of both groups showed no significant differences. Thus, the consumption of L. plantarum Dad-13 increased weight, height, and BMI but could not influence the numbers of gut microbiota, SCFA, and the fecal characteristics of healthy adolescents.


Keywords


Lactobacillus plantarum Dad-13; Gut Microbiota; Short Chain Fatty Acid; Fecal Characteristics

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DOI: https://doi.org/10.22146/ifnp.86598

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