The Consumption Effect of Indigenous Probiotic Powder Lactobacillus plantarum Dad-13 on Gut Microbiota Population and Short Chain Fatty Acids in Students of SMPN 1 Pangururan, Samosir

https://doi.org/10.22146/ifnp.86598

Nancy Eka Putri Manurung(1), Pratama Nur Hasan(2), Mohammad Juffrie(3), Tyas Utami(4), Rini Yanti(5), Endang Sutriswati Rahayu(6*)

(1) Faculty of Agricultural Technology, Gadjah Mada University
(2) Faculty of Agricultural Technology, Universitas Gadjah Mada
(3) Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
(4) Faculty of Agricultural Technology, Universitas Gadjah Mada
(5) Faculty of Agricultural Technology, Universitas Gadjah Mada
(6) Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The human intestine is a diverse ecosystem populated by microbiota affected by several factors, including age. The aim of this research was to evaluate the effects of the probiotic powder Lactobacillus plantarum Dad-13 on the numbers of gut microbiota, short-chain fatty acids (SCFA), and fecal characteristics in healthy adolescents. This research was conducted at SMPN 1 Pangururan, Samosir, with a randomized, double-blind, parallel placebo-controlled trial. 54 healthy adolescents aged 13 to 14 were divided into two groups, one consumed a gram of skimmed milk powder (placebo group) and the other ingested powder containing L. plantarum Dad-13 with 1.18 × 109 CFU/gram (probiotic group). After 33 days of intervention, the height of placebo group (149.42 ± 5.03 cm) and probiotic group (154.37 ± 4.67 cm) increased significantly. Significant increases in body weight (44.35 kg ± 4.61 to 45.20 kg ± 4.78) and BMI (and 18.77 ± 2.12 to 18.99 ± 2.11) were observed in the probiotic group. In the probiotic group, the numbers of gut microbiota were not significantly affected (p > 0.05). The amount of SCFA and fecal characteristics of both groups showed no significant differences. Thus, the consumption of L. plantarum Dad-13 increased weight, height, and BMI but could not influence the numbers of gut microbiota, SCFA, and the fecal characteristics of healthy adolescents.


Keywords


Lactobacillus plantarum Dad-13; Gut Microbiota; Short Chain Fatty Acid; Fecal Characteristics

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References

Agans, R., Rigsbee, L., Kenche, H., Michail, S., Khamis, H.J., and Paliy, O. (2011). Distal gut microbiota of adolescent children is different from that of adults. FEMS Microbiology Ecology, 77(2), 404–412, https://doi.org/10.1111/j.1574-6941.2011.01120.x.

Angelakis, E., Bastelica, D., Ben, A., El, A., Dutour, A., Mege, J., Alessi, M., and Raoult, D. (2012). Microbial Pathogenesis An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice. Microbial Pathogenesis, 52(1), 61–68, https://doi.org/10.1016/
j.micpath.2011.10.004.

Anonim. (2017). Konsumsi Kalori Dan Protein Penduduk Indonesia Dan Provinsi 2017. Jakarta: BPS-Statistic Indonesia

Anonim. (2018). Konsumsi Kalori Dan Protein Penduduk Indonesia Dan Provinsi 2018. Jakarta: BPS-Statistic Indonesia https://doi.org/3202001.

Anonymous. (2014). Total Diet Study: 2014 Indonesian Individual Food Consumption Survey. pp.

De Filippo, C., Cavalieri, D., Di Paola, M., Ramazzotti, M., Poullet, J. B., Massart, S., Collini, S., Pieraccini, G., & Lionetti, P. (2010). Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proceedings of the National Academy of Sciences of the United States of America, 107(33), 14691–14696. https://doi.org/
10.1073/pnas.1005963107

FAO and WHO. (2011). Probiotics in Food: Health and Nutritional Properties and Guidelines for Evaluation. Rome, pp.

Finegold, M., Attebery, H., and Sutter, V. L. (1974). Effect of diet on human comparison of Japanese fecal and flora: American. The American Journal of Clinical Nutrition, 27(12), 1456–1469, https://doi.org/10.1093/
ajcn/27.12.1456

Fitrianingthias, R. D. R., Rahayu, E. S., Utami, T., Widada, J (2018). The effect of consumption indigenous probiotic powder Lactobacillus plantarum Mut-7 on the population of Lactobacillus, Bifidobacterium, Clostridium, and Short Chain Fatty Acids on Feces of Junior High School Student at Ngemplak. Universitas Gadjah Mada.

Glibowski, P., & Turczyn, A. (2013). Determining the effect of consuming fermented milk drinks on the incidence of constipation, diarrhea, and resistance to respiratory illness. Roczniki Panstwowego Zakladu Higieny, 64(4), 339–344.

Goossens, D., Jonkers, D., Russel, M., Stobberingh, E., Van Den Bogaard, A., & StockbrUgger, R. (2003). The effect of Lactobacillus plantarum 299v on the bacterial composition and metabolic activity in faeces of healthy volunteers: a placebo-controlled study on the onset and duration of effects. Alimentary pharmacology & therapeutics, 18(5), 495–505. https://doi.org/10.1046/j.1365-2036.2003.01708.x

Guerin-Danan, C., Chabanet, C., Pedone, C., Popot, F., Vaissade, P., Bouley, C., Szylit, O., and Andrieux, C. (1998). Milk fermented with yogurt cultures and Lactobacillus casei compared with yogurt and gelled milk: Influence on intestinal microflora in healthy infants. American Journal of Clinical Nutrition, 67(1), 111–117, https://doi.org/10.1093/ajcn/67.1.111.

Hentges D. J., B. R. Maier, G. C. Burton, M. A. Flynn, and R. K. T. (1977). Effect of a High-Beef Diet on the Fecal Bacterial Flora of Humans. Cancer Research, 37(2), 568–571.

Indonesian Central Agency of Statistics. (2022). Jumlah Kasus Penyakit Menurut Kabupaten/Kota dan Jenis Penyakit di Provinsi Sumatera Utara, 2022. Retrieved December 18, 2023. https://sumut.bps.go.id/
statictable/2023/03/30/3064/jumlah-kasus-penyakit-menurut-kabupaten-kota-dan-jenis-penyakit-di-provinsi-sumatera-utara-2022.html.

Kadooka, Y., Sato, M., Imaizumi, K., Ogawa, A., Ikuyama, K., Akai, Y., Okano, M., Kagoshima, M., and Tsuchida, T. (2010). Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial. European Journal of Clinical Nutrition, 64(6), 636–643, https://doi.org/10.1038/ejcn.2010.19.

Kleerebezem, M., and Vaughan, E. E. (2009). Probiotic and Gut Lactobacilli and Bifidobacteria: Molecular Approaches to Study Diversity and Activity. Annual Review of Microbiology, 63(1), 269–290, https://doi.org/10.1146/annurev.micro.091208.073341.

Kurakawa, T., Ogata, K., Matsuda, K., and Tsuji, H. (2015). Diversity of Intestinal Clostridium coccoides Group in the Japanese Population, as Demonstrated by Reverse Transcription- Quantitative PCR. PLoS ONE, https://doi.org/10.1371/journal.pone.0126226.

Levison, M. E. (1973). Effect of colon flora and short chain fatty acids on growth in vitro of Pseudomonas aeruginosa and Enterobacteriaceae. Infection and Immunity, 8(1), 30–35.

Marta, M., E. S. Rahayu, J. W. (2018). Pengaruh konsumsi probiotik powder Lactobacillus plantarum Dad-13 terhadap populasi Lactobacillus, Bifidobacterium, Coliform, dan Konsentrasi Shirt Chain Fatty Acid pada Feses pelajar di SMPN 1 Ngemplak Yogyakarta. Universitas Gadjah Mada p.

Matsuki, T., Watanabe, K., Fujimoto, J., Takada, T., Tanaka, R., and T. (2004). Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Applied and Environmental Microbiology, 70(12), 7220–7228, https://doi.org/10.1128/AEM.70.12.7220.

Matsumoto, K., Takada, T., Shimizu, K., Moriyama, K., Kawakami, K., Hirano, K., Kajimoto, O., & Nomoto, K. (2010). Effects of a probiotic fermented milk beverage containing Lactobacillus casei strain Shirota on defecation frequency, intestinal microbiota, and the intestinal environment of healthy individuals with soft stools. Journal of bioscience and bioengineering, 110(5), 547–552. https://doi.org/
10.1016/j.jbiosc.2010.05.016

Matsumoto, K., T. Takada, K. Shimizu, Y. Kado, K. Kawakami, I. Makino, Y. Yamaoka, K. Hirano, A. Nishimura, O. Kajimoto, and K. N. (2006). The Effects of a Probiotic Milk Product Containing Lactobacillus casei Strain Shirota on the Defecation Frequency and the Intestinal Microflora of Sub-optimal Health State Volunteers: A Randomized Placebo-controlled Cross-over Study. Bioscience Microflora, 25(2), 39–48.

McCabe, L. R., Irwin, R., Schaefer, L., & Britton, R. A. (2013). Probiotic Use Decreases Intestinal Inflammation and Increases Bone Density in Healthy Male but not Female Mice. National Institute of Health, 228(8), 1793–1798, https://doi.org/10.1002/jcp.24340.
Probiotic.

Nakayama, J., A. Yamamoto, L. A. Palermo-Conde, K. Higashi, K. Sonomoto, J. Tan, and Y. K. L. (2017). Impact of westernized diet on gut microbiota in children on Leyte Island. Frontiers in Microbiology, 8, 1–18, https://doi.org/10.3389/fmicb.2017.00197.

Nakayama, J., K. Wanabe, J. Jiang, K. Matsuda, S. H. Chao, P. Haryono, O. La-ongkham, M. A. Sarwoko, I. N. Sujaya, L. Zhao, K. T. Chen, H. H. Chiu, T. Hidaka, N. X. Huang, C. Kiyohara, T. Kurakawa, N. Sakamoto, K. Sonomoto, K. Tashiro, H. Tsuji, M. J. Chen, and Y. K. L. (2015). Diversity in gut bacterial community of school-age children in Asia. Scientific Reports, 5, 1–11, https://doi.org/10.1038/srep08397.

Onubi, O. J., A. S. Poobalan, B. Dineen, D. Marais, and G. McNeill. (2015). Effects of probiotics on child growth: a systematic review. Journal of Health, Population and Nutrition, 34(1), 8, https://doi.org/10.1186/
s41043-015-0010-4.

Panda, S., I. E. Khader, F. Casellas, J. L. Vivancos, M. G. Cors, A. Santiago, S. Cuenca, F. Guarner, and C.M. (2014). Short-term effect of antibiotics on human gut microbiota. PLoS ONE, 9(4) https://doi.org/
10.1371/journal.pone.0095476.

Panjaitan, M., Rahayu, E. S., Widada, J., Utami, T., and Cahyanto, M. (2018). Pengaruh Konsumsi Probiotik Powder Lactobacillus plantarum Dad-13 Pada Populasi Lactobacillus plantarum, Bacteroides fragilis, Prevotella, dan Senyawa Putrefactive dari Feses Siswa SMPN 1 Ngemplak. Universitas Gadjah Mada.

Rahayu, E. S., Yogeswara, A., Mariyatun, Windiarti, L., Utami, T., and Watanabe, K. (2015). Molecular characteristics of indigenous probiotic strains from Indonesia. International Journal of Probiotics and Prebiotics, 11(2).

Rios-Covian, D., P. Ruas-Madiedo, A. Margolles., M. Gueimonde, C. G. de los Reyes-Gavilan, and N.S. (2009). Efecto de un programa integrado de mindfulness e inteligencia emocional sobre las estrategias cognitivas de regulación emocional. Ansiedad y Estres, 15(2–3), 207–216, https://doi.org/10.3389/fmicb.2016.00185.

Sepp, E., I. Smidt, J. Stesepetova, T. Roop, P. Hutt, M. Ratsep, M. M. (2018). The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial. Journal of Functional Foods, 48(April), 430–438, https://doi.org/10.1016/
j.jff.2018.04.053.

Shioiri, T., Yahagi, K., Nakayama, S., Asahara, T., Yuki, N., Kawakami, K, Yamaoka, Y., Sakai, Y., Nomoto, K., and Totani, M. (2006). The Effects of a Synbiotic Fermented Milk Beverage Containing Lactobacillus casei Strain Shirota and Transgalactosylated Oligosaccharides on Defecation Frequency, Intestinal Microflora, Organic Acid Concentrations, and Putrefactive Metabolites of Sub-Opti. Bioscience and Microflora, 25(4), 137–146, https://doi.org/
10.12938/bifidus.25.137.

Silva, J. R., Silva, J. B. da., Costa, G. N., Santos, J. S. dos., & Castro-Gomez, R. J. H. (2020). Probiotic gummy candy with xylitol: development and potential inhibition of Streptococcus mutans UA 159. Research, Society and Development, 9(10), e7369108942. https://doi.org/10.33448/rsd-v9i10.8942

Tari, A. I. N., Handayani, C. B., and Sudarmi (2016). Potensi Probiotik Indigenus Lactobacillus plantarum Dad 13 pada Yogurt Dengan Suplementasi Ekstrak Ubi Jalar Ungu untuk Penurun Diare dan Radikal Bebas. Agritech, 36(1), 7–14, https://doi.org/10.1109/
ITHET.2012.6246063.

Toscano, M., Grandi, R. De, Leonardo, V., Mattina, R., and Drago, L. (2017). Ability of Lactobacillus kefiri LKF01 (DSM32079) to colonize the intestinal environment and modify the gut microbiota composition of healthy individuals. Digestive and Liver Disease, 49(3), 261–267, https://doi.org/
10.1016/j.dld.2016.11.011.

Utami, T., Kasmiati, Harmayani, E., and Rahayu, E. S. (2015). Recovery of Lactobacillus casei strain shirota (LCS) from the intestine of healthy Indonesian volunteers after intake of fermented milk and its impact on the enterobacteriaceae faecal microbiota. International Journal of Probiotics and Prebiotics, 10(2–3), 77–84,.

Wang, L., Zhang, J., Gui, Z., Kwok, L., Ma, C., Zhang, W., Lv, Q., Weiqiang, H. & Zhang, H. (2014). Effect of oral consumption of probiotic Lactobacillus plantarum P-8 on fecal microbiota, SIgA, SCFAs, and TBAs of adults of different ages. Nutrition, 30(7–8), 776–783, https://doi.org/10.1016/j.nut.2013.11.018.

Wu, G. D., J. Chen, C. Hoffmann, K. Bittinger, Y. Chen, and A. S. (2011). Linking long-term dietary patterns with gut microbial enterotypes. Science, 334(6052), 105–108, https://doi.org/10.1126/science.1208344.



DOI: https://doi.org/10.22146/ifnp.86598

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