Chemical Characteristics of Waru Leaf (Hibiscus tiliaceus) As Food Packaging Material

https://doi.org/10.22146/ifnp.76137

Angela Firdausa Alif Iskandar(1*), Umar Santoso(2), Supriyadi Supriyadi(3)

(1) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada
(2) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada
(3) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Waru leaf (Hibiscus tilaceus) is commonly used as a traditional food packaging material in Indonesia. Waru leaf is known to contain bioactive components with antioxidant and antimicrobial activity. This research aimed to determine the chemical properties of waru leaf of various maturity levels and provide information on the potency of leaf extract that can be used as active packaging additive. The chemical properties include fatty acids, volatile compounds, antioxidant activity, and total phenolic content. It was discovered that both the young and mature waru leaf contained 4 types of fatty acids: predominantly linoleic acid and linolenic acid. In the young waru leaf, the primary volatile compounds were acids, with cis-vaccenic acid (21%) and glycidyl palmitate comprising (16.8%). As for the mature leaf, the main volatile compounds were acids and esters, with glycidyl oleate at (18.52%), glycidyl palmitate (12.28%), and Decocanoic acid, 3-hydroxy- (7.85%). The crude methanol extract was fractionated with hexane, ethyl acetate, and butanol to analyze bioactive compounds. The highest antioxidant activity was found in ethyl acetate fraction with an average of %Radical Scavenging Activity (%RSA) of 36.25% on the young waru leaf and 29.92% on the mature waru leaf. The ethyl acetate fraction had the highest total phenolic content of 164.66 mg GAE/g and 146.50 mg GAE/g for young and mature waru leaves, respectively. The result shows that waru leaf extract is a potential ingredient for developing active packaging. 


Keywords


antioxidant; bioactive; food packaging material; total phenolic; waru leaf

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References

Abqoriyah, R. Utomo, dan B. Suwignyo. (2015). Produktivitas tanaman kaliandra (Calliandra calothyrsus) sebagai hijauan pakan pada umur pemotongan yang berbeda. Buletin Penelitian, 39, 103-108. https:// doi.org/10.21059/buletinpeternak.v39i2.6714.

Abreu, D. A. Pereira De, J. M. Cruz and P. Paseiro Losada. (2012). Active and Intelligent Packaging for the Food Industry. Food Reviews International, 28, 146–187. https://doi.org/10.1080/87559129.2011.595022

AOAC. (2005). Official Methods of Analysis. Association of Official Analytical Chemists. Editor:

Horwitz, W and G. W. Latimer, Jr. Published by AOAC International. 18th Edition. USA.

Aree, Thammarat and Suchada Jongrungruangchok. (2016). Enhancement of Antioxidant Activity of Green Tea Epicatechins in-Cyclodextrin Cavity: Single Crystal X-Ray Analysis, DFT Calculation and DPPH Assay. Carbohydrate Polymers, 151, 1139–1151. https://doi.org/10.1016/j.carbpol.2016.05.113

Casey, N. (2015). Plant leaf in food preparation and packaging. Utar Agriculture Science Journal, 1 (4), 34–39.

Firdausi, Ismawati, Rurini Retnowati dan Sutrisno. (2015). Fraksinasi Ekstrak Metanol Daun Mangga Kasturi (Mangifera casturi kosterm) dengan Pelarut N-Butanol. Kimia Student Journal, 1(1), 785 – 790.

Grover,N. and V. Patni. (2013). Phytochemical characterization using various solvent extracts and GC-MS analysis of methanolic extract of Woodfordia fruticosa (L) Kurz. leaf. Int J Pharm Pharm Sci, 5, 291–295.

Koten, B & Soetrisno, R & Ngadiyono, N & Soewignyo, B. (2017). Penampilan Produksi Hijauan Hasil Tumpangsari Arbila (Phaseolus lunatus) Berinokulum Rhizobium dan Sorgum (Sorghum bicolor) pada Jarak Tanam Arbila dan Jumlah Baris Sorgum. Sains Peternakan. 12, 26. 10.20961/sainspet.11.1.26-33.

Kuo, J. M., A. Hwang, D. B. Yeh, M. H. Pan, M. L. Tsai, dan B.S. Pan. (2006). Lipoxygenase from Banana Leaf: Purification and Characterization of an Enzyme That Catalyses Linoleic Acid Oxygenation at the 9-Position. J. Agric Food Chem, 54, 3151-31-56.

Kusumowati, I.T.D., Sudjono, T.A., Suhendi, A. Da’i, M. & Wirawati, R. (2012). Korelasi kandungan fenolik dan aktivitas antiradical ekstrak etanol daun empat tanaman obat Indonesia (Piper bettle, Sauropus androgynous, Averrhoa bilimbi, dan Guazuma ulmifolia). Pharmacon 13(1): 1-5. doi:10.23917/pharmacon.v13i1.19.

Lazo, O., Guerrero, L., Alexi, N., Grigorakis, K., Claret, A., Perez, Z.A. dan Bou, R. (2017). Sensory Characterization, Physico-Chemical Properties and Somatic Yields of Five Emerging Fish Species. Food Research International 100, 396-406. https://doi.org/10.1016/j.foodres.2017.07.023

Meenashree, B., Vasanthi, V.J. & Mary, R.N.I. (2014). Evaluation of total phenolic content and antimicrobial activities exhibited by the leaf extracts of Musa acuminate (banana). International Journal of Current Microbiology and Applied Sciences, 3(5), 136-141.

Nayeem, Naira and Karvekar, M. D. (2010). Comparative Phytochemical and Pharmacological Screening of the Methanolic Extracts of the Frontal and Mature Leaf of Tectona Grandis. International Journal of Pharma and Bio Sciences, 1(3), 25.

Nandagopalan, V., M. Johnson Gritto and A. Doss. (2015). GC-MS Analysis of Bioactive Components of the Methanol Extract of Hibiscus tiliaceus L. Asian J. Plant Sci. Res., 5(3), 6–10.

Desai, Nivas & Sonar, Babu & Shaikh, Suraj & Patil, Udaysing & Gaikwad, Dattatraya & Chavan, Niranjana & Sabale, Anjali & Chavan, P. (2010). Screening of Some Coastal Plant Resources for Their Antioxidant Potential, Total Polyphenol and Flavonoid Content. Phcog J. 2, 151–156. 10.1016/S0975-3575(10)80083-5.

Noviadji, B.R. (2014). Desain kemasan tradisional dalam konteks kekinian. Jurnal Fakultas Desain, 1(1): 10-21. https://doi.org/10.34148/artika.v1i1.24

Nugraheni, T. P., Rosvita, V. & Pratiwi, H. K. (2017). Uji aktivitas penangkapan radikal bebas DPPH oleh ekstrak etanol daun pisang Tanduk (Musa paradisiaca Var. Formatypica) dan dan pisang Cavendish (Musa paradisiaca Var. Sapientum). Indonesia Jurnal Farmasi, 2(1), 37-42.

Opara, Umezuruike Linus, and Asanda Mditshwa. (2013). A review on the role of packaging in securing food system: Adding value to food products and reducing losses and waste. African Journal of Agriculture Research, 2621-2630. Doi: 10.5897/AJAR2013.6931

Perez, C. Rodriguez, R. Quirantes-Pine, A. Fernández-Gutiérrez and Segura Carretero. (2015). Optimization of Extraction Method to Obtain a Phenolic Compounds-Rich Extract from Moringa oleifera Lam Leaves. Industrial Crops and Products, 66, 246–254. https://doi.org/10.1016/j.indcrop.2015.01.002

Ramproshad, S., T. Afroz, B. Mondal, A. Haque, S. Ara, R. Khan, S. Ahmed. (2012). Antioxidant and Antimicrobial Activities of Leaves of Medicinal Plant Hibiscus tiliaceus L. Pharmacology Online, 82-87.

Risch, S. J. (2009). Food Packaging History and Innovations. J. Agric. Food Chem., 57, 8089–8092. https://doi.org/10.1021/jf900040r

Salisbury. (1995). Fisiologi tumbuhan jilid 2. Bandung: ITB.

Sari, Diah Ayu Puspa. (2017). Pengaruh Jenis Bahan Pengemas terhadap Aktivitas Antioksidan, Kadar Lemak, dan Kadar Abu Tempe Kedelai Kuning Impor dan Lokal. Doctoral dissertation, Universitas Gadjah Mada.

Shibahara, A., Yamamoto, K., Nakayama, T. and Kajimoto, G. (1986). Cis-Vaccenic acid in mango pulp lipids. Lipids, 21(6), 388-394. https://doi.org/10.1007/BF02534933

Shibahara, A., Yamamoto, K., Nakayama, T. and Kajimoto, G. (1987). Cis-Vaccenic acid in pulp lipids of commonly available fruits. Journal of the American Oil Chemists’ Society, 64(3), 397-401. https://doi.org/10.1007/BF02549303

Socaci, Sonia Ancuta., Maria Tofana., Carmen Socaciu., Cristina Semeniuc. (2009). Optimization of HS/GC-MS Method for the Determination of Volatile Compounds from Indigenous Rosemary. Journal of Agroalimentary Processes and Technologies, 15 (1), 45-46.

Susanto, A. (2008). Kadar Klorofil Pada Berbagai Tanaman yang Berbeda Umur. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam. Surabaya: Universitas Negeri Surabaya.

Taveira, M., F. Fernandes, P. Guedes de Pinho, P.B. Andrade, José A. Pereira, P. Valentão. (2009). Evolution of Brassica rapa var. rapa L. volatile composition by HS-SPME and GC/IT-MS. Microchem. J, 93, 140–146. https://doi.org/10.1016/j.microc.2009.05.011

Wikanta, T., Gusmita, D., Rahayu, L. dan Marraskuranto, E. (2012). Kajian Awal Bioaktivitas Ekstrak Etanol dan Fraksinya dari Spons Callyspongia sp. Terhadap Sel Lestari Tumor HeLa. JPB Perikanan 7(1): 1-10. https://doi.org/10.15578/jpbkp.v7i1.64

Zhang, M. P., Zhang, C. J., Yu, G. H., Jiang, Y. Z., Strasser, R. J., Yuan, Z. Y., Yang, X. S. and Chen, G. X. (2010). Changes in chloroplast ultrastructure, fatty acid components of the thylakoid membrane and chlorophyll a fluorescence transient in flag leaf of super-high yield hybrid rice and its parents during the reproductive stage. Journal of plant physiology, 167(4), 277–285. https://doi.org/10.1016/j.jplph.2009.09.017



DOI: https://doi.org/10.22146/ifnp.76137

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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