Binding of Aflatoxin B1 to Lactobacillus paracasei SNP-2 and Stability of Bacteria-AFB1 Complex

https://doi.org/10.22146/ifnp.24258

Rohula Utami(1), Tyas Utami(2), Suparmo Suparmo(3), Endang Sutriswati Rahayu(4*)

(1) Department of Food Science and Technology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia
(2) Departement of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, 5528, Indonesia
(3) Departement of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, 5528, Indonesia
(4) Departemnt of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, 5528, Indonesia
(*) Corresponding Author

Abstract


The aim of this research was to study the binding ability of viable and non-viable of Lactobacillus paracasei SNP-2 to aflatoxin B1 (AFB1) in phosphate buffer saline (PBS) at pH 7.3. Bacterial cells were grown in MRS broth at 37 °C for 24 h, and then centrifuged at 1,800g[a1]  for 20 min at 10 °C to get the pellet. Pellet was suspended in PBS pH 7.3 until the cell concentration was about 1010 CFU/ml. Viable cells, the heated, and acid-killed cells were evaluated their ability to bind AFB1 in PBS pH 7.3. Stability of the L. paracasei SNP-2/AFB1 complexes was evaluated by determining the amount of the released AFB1 to the PBS following five times washing. The results showed that AFB1 binding ability to heat-and acid-kill bacteria were higher than that of by viable cells. More than 70% of bound AFB1 was released from viable bacteria after five times washing. However, the heated and acid-killed cell treatments significantly increased the complex stability of bacteria-AFB1

Keywords


Aflatoxin B1; Lactobacillus acidophilus SNP-2; Binding of AFB1; Peptidoglycan

Full Text:

PDF


References

Coulombe, JR. R.A. 1993. Symposium: Biological Actions of Mycotoxins. J. Dairy Sci.76: 880-889

Dharmaputra, O. S., Retnowati, I., Ambarwati, S., and Maysra, E. 2005. Aspergillus flavus Infection and Aflatoxin Contamination in Peanuts at Various Stages of Delivery Chains in Cianjur Regency, West Java, Indonesia. Biotropia. 24:1-19

El-Nezami, H., Kankaanpaa, P., Salminen, S., and Ahokas, J. 1998a. Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen, aflatoxin B1. J. Food Chem. Toxicol. 36: 321-326

El-Nezami, H., Kankaanpaa, P., Salminen, S., and Ahokas, J. 1998b. Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media. J. Food Protection. 61:466-468.

Haskard, C. A., El-Nezami, H., Kankaanpaa, P. E., Selminen, S., and Ahokas. J. T. 2001. Surface binding of aflatoxin B1 by lactic acid bacteria. Applied and Environmental Microbiology. 67(7): 3086-3091.

Hernandez-Mendoza. A., Garcia, H. S., Steele, J. L. 2009. Screening of Lactobacillus casei strains for their ability to bind aflatoxins B1. F. Chem. Toxicol. 47: 1064-1048.

Lahtinen, S. J., Haskard, C. A., Ouwehand, A. C., Salminen, S. J., and Ahokas. J. T. 2004. Binding of aflatoxin B1 to cell wall components of Lactobacillus rhamnosusstrain GG. Food Additives and Contaminants. 21(2):158-164.

Oatley, J. T., Rarick, M. D., Ji, G. E, and Linz, J. E. 2000. Binding of aflatoxin B1 to Bifidobacteria in vitro. Journal of Food Protection. 63(8): 1133-1136.

Peltonen, K., El-Nezami, H., Haskard, C., Ahokas, J. and Salminen, S. 2001. Aflatoxin B1 binding by dairy strain lactic acid bacteria and Bifidobacteria. Journal of Dairy Science 84: 2152-2156.

Rahayu, E.S., Raharjo, S., Rahmianna, A.A. 2003. Aflatoxin contamination in corn production in East Java. Agritech 23 (4):174.

Samarajeewa, U. S., Cohen, A. C., and Wei, C. L. 1990. Detoxification of aflatoxin in foods and feeds by physical and chemical methods. J. Food Protection. 53: 489-501.

Simanjuntak, R. 2005. Decontamination of aflatoxin B1 by binding to lactic acid bacteria. M.Sc. Thesis in the Study Program of Food Science and Technology, Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia. pp. 59.

Vosough, P.R., and Sani, A.M. 2014. In vitro effect of Lactobacillus rhamnosusGG on reduction of aflatoxin B1. Nutrition Food Sci. 44: 32-40.

William, J. H., Phillips, T. D., Jolly, P. E., Stiles, J. K., Jolly, C. M., and Aggarwal, D. 2004. Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. American Journal of Clinical Nutrition. 80: 1106-1122.

Zinedine, A., Faid, M., and Benlemlih. M. 2005. In vitro reduction of aflatoxin B1 by strain of lactic acid bacteria isolated from Morocca Sourdough bread. Int. J. Agriculture and Biology. 7 (1): 67-70.



DOI: https://doi.org/10.22146/ifnp.24258

Article Metrics

Abstract views : 2132 | views : 2382

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

Journal of Indonesian Food and Nutrition Progress have been indexed by: 

   

 

This works is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.