* Corresponding Author
Anti-Autooxidative and Anti-Photooxidative Effect of Lemon Grass Extracts (Cymbopogon citratus)
https://doi.org/10.22146/jifnp.31 Sri Raharjo(1*), Edi Suryanto(2)
(1) Gadjah Mada University
(2) Sam Ratulangi University
(*) Corresponding Author
Abstract
Lemon grass (Cymbopogon citratus) is a traditional food ingredient characterized by its specific and refreshing aroma. This study was intended determine to the effect of lemon grass extract in both autooxidation and photooxidation reaction in model systems. Lemon grass was extracted sequentially with hexane, acetone and ethanol. The antioxidative effects of the extracts were determined by 1,1-diphenyl-2-spicrylhydrazyl (DPPH) scavenging assay, reducing power, β-carotene bleaching method and linoleic acid system. The photooxidation reaction system was consisted of linoleic acid (0,03 M) in methanol containing 100 ppm erythrosine as a sensitizer and the reaction mixture was exposed under 4000 lux fluorescent light for up to 5 hours. Total phenolic content of acetone, hexane and ethanol extracts were 20.38, 7.65, and 4.97 mg/100g, respectively, which was expressed as gallic acid equivalent. The addition of acetone extracts of lemon grass at 200 and 500 ppm in the reaction mixture showed the highest scavenging activity in 1,1-diphenyl-2-spicrylhydrazyl (DPPH) radical, reducing power, and β-carotene bleaching and linoleic acid system.
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Burda, S., and W. Oleszek. 2001. Antioxidant and Antiradical Activities of Flavonoids. J. Agric. Food Chem. 49: 2774-2779.
Chipault, J. R., G. R. Mizuno, Hawkinsand, and W. Lundberg. 1955. Antioxidant Properties of Natural Spices. Food Research. 17: 46-55
Duh, P. D. and G. C. Yen. 1997 Antioxidative Activity of Three Herbal Water Extracts. Food Chemistry. 60: 639-645
Endo, Y., R. Usuki, and T. Kaneda. 1985. Antioxidant Effects on Chlophylls and Pheophytin on the Autooxidation of Oils in The Dark II. JAOCS. 62: 1387-1390
Hammerschmidt, P. A., and D. E. Pratt., 1978. Phenolic Antioxidant of Dried Soybeans. J. Food Sci. 43:556-559
Hung and Yen. 2002. Antioxidant Activity of Phenolic Compounds Isolated from Meson procumbens Hemsl. J. Agric Food Chem. 50: 2993-2997
Lai, L-S., S-T Chou, and W-W Chao 2001. Studies on the Antioxidative Activities of Hsian-tsao (Mesonaprocumberns Hemsl) Leaf GUm. J. Agric Food Chem. 49: 963-968
Lee, K.H., M. Y. Jung, and S. Y. Kim. 1997. Quenching Mechanism and Kinetics of Ascorbyl Palmitate for Reduction of Photosensitized Oxidation of Oils. JAOCS. 74: 1053-1057
Miean, K. H. and S. Mohamed. 2001. Flavonoid (Myricetin, Quercetin, Kaemprefol, Luteolin, and Apigenin) Content of Edible Troical Plants. J. Agric Food Chem. 49:3106-3112
Morel, I., G. Lescoat, P. Cogrel, O. Sergent, N. Pasdecoup, P. Brissot, P. Cillard, and J. JIllard 1993. Antioxidant and Iron Chelating Activities of The flavonoids Catechin, Quercetin and Diosmetin on Iron-Loaded Rat Hepatocyte Cultures. Biochem. Pharmacol. 1: 13-19
Peri, C. and Pompei C. 1971. Estimation of Different Phenolic Groups in Vegetable Extracts. Phytochemistry. 10:2187-2189
Perry, L. M. 1978. Medical Plants of East and ANtioxidant. In Simic, M. G. Karel M. (eds) Autooxidation in Food and Biological Systems, Plenum Press, New York
Salah, N., N. J. Miller, G. Paganga, L. Tijburg, G. P. Bolwell, C. Rice-Evans. 1995. Polyphenolic Flavonols as Scavenger of Aquesous hase Radicals and as Chain-Breaking Antioxidants. Arch Biochim. Biophys. 2: 339-346
Sorata, Y., U. Takahama, and M. Kimura. 1984. Protective Effect of Quercetin and Rutin on Photosensitized Lysis of Human Erythrocytes in The presence of Hematoporphyrin. Biochim. Biophys. Acta. 799: 313-317
Von Gadow, A., E. Joubert, and C. F. Hansmann. 1997.Comparison of the Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products. J. Agric Food Chem. 46:4113-4117
Velioglu, Y. S., G. Mazza, L. Gao, and B. D Oomah. 1998. Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products. J. Agric Food Chem. 46:4113-4117
Yang, W. T., J. H. Lee, and D. B. Min. 2002. Quenching Mechanisms and Kinetics of a-Toopherol and b-Carotene on the Photosensiting Effect of Synthetic Food Colorant FD&C Red No. 3. J. Food Sci. 67: 507-510
Yen, G-C, and H-Y. Chen 1995. Antioxidant Activity of Various Tea Extracts in Relation to Their Antimutagenicity. J. Agric Food Chem. 43:27-32.
Wanasundara, U. N., and F. Shahidi. 1998. Antioxidant and Pro-oxidant Activity of Green Tea Extract in Marine Oils. Food Chemistry. 63: 335-342
Whang, P. F., and R. L. Zheng. 1992. Inhibitions of The autoxidation of Linoleic Acids Flavonoids on Micelles. Chem. Phys. Lipids. 63: 37-40
Wiart, C. 2002. Medical Plants of Southeast Asia. Prentice Hall, Malaysia
DOI: https://doi.org/10.22146/jifnp.31
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