Isolation and Identification of Antiradical and Anti-photooxidant Component of Aloe vera chinensis

https://doi.org/10.22146/jifnp.30

Yohana S. Kusuma Dewi(1*), Tranggono Tranggono(2), Sri Raharjo(3), Puji Hastuti(4)

(1) Tanjungpura University
(2) Gadjah Mada University
(3) Gadjah Mada University
(4) Gadjah Mada University
(*) Corresponding Author

Abstract


A potent antiradical and antiphotooxidant compound from Aloe vera chinensis was investigated. The results indicated that the methanolic extracts produced stronger antiradical and antiphotoaxidant activity also gave higher yields of extract than other organic solvents. Silica gel column separated methanolic extracts into five fractions. Of the five fractions, fraction II possessed significant antiradical activity (70.2 % inhibition on free radical DPPH) and its showed significant antiphotooxidant activity (60.1 % inhibition on hydroperoxide formation). Subsequently, fraction II was separated into eight subfractions by silica gel column. The sub fraction II-b exhibited stronger antiradical and antiphotooxidant activity than others and showed a single spot in thin layer chromatography Silica gel 60 F254 plates. On the basis of mass spectrophotometry in combination with reversed phased, high performance chromatography, this compound has been identified as 1,8 dihydroxy-3- hydroxymethylanthraquinone (Aloe emodin).


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DOI: https://doi.org/10.22146/jifnp.30

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Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

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