Isolation and Identification of Antiradical and Anti-photooxidant Component of Aloe vera chinensis

https://doi.org/10.22146/jifnp.30

Yohana S. Kusuma Dewi(1*), Tranggono Tranggono(2), Sri Raharjo(3), Puji Hastuti(4)

(1) Tanjungpura University
(2) Gadjah Mada University
(3) Gadjah Mada University
(4) Gadjah Mada University
(*) Corresponding Author

Abstract


A potent antiradical and antiphotooxidant compound from Aloe vera chinensis was investigated. The results indicated that the methanolic extracts produced stronger antiradical and antiphotoaxidant activity also gave higher yields of extract than other organic solvents. Silica gel column separated methanolic extracts into five fractions. Of the five fractions, fraction II possessed significant antiradical activity (70.2 % inhibition on free radical DPPH) and its showed significant antiphotooxidant activity (60.1 % inhibition on hydroperoxide formation). Subsequently, fraction II was separated into eight subfractions by silica gel column. The sub fraction II-b exhibited stronger antiradical and antiphotooxidant activity than others and showed a single spot in thin layer chromatography Silica gel 60 F254 plates. On the basis of mass spectrophotometry in combination with reversed phased, high performance chromatography, this compound has been identified as 1,8 dihydroxy-3- hydroxymethylanthraquinone (Aloe emodin).


Full Text:

PDF


References

AOCS. 1990. Official. Methods and Recommended of the American Oil Chemist Society. 4th Ed. Champaign, II. American Oil Chemist Society.

Dewi, Y. S. K., Tranggono, S. Raharjo and Pudji Hastuti. 2005. Extract Aloe vera Sebagai Penangkap Radikal. Agritech (In Press).

Duh, P. D. 1998. Antioxidant Activity of Burdock (Actium lappa Linne'): Its Scavenging Effect on Free-Radical and Active Oxygen. J. Am. Oil. Chem. Soc. 75:455-461

Esteban A>, J. M. Zapata, L. Casano M. Martin dan B. Sabater. 2000. Peroxide Activity in Aloe barbadensis Comemercial Gel: Probable Role in Skin Protection. Planta Medica 66: 724-727.

Grindlay, D. and T. Reynolds. 1986. The Aloe Vera Phenomenon: A Review on The Properties and Modern Uses of The Leaf Parenchyme Gel. J. Ethnopharmacol. 16: 117-151

Guyid, S., N. Marnet, D. Laraba., P. Sannoner, J. F. Drilleau. 1998. Reversed Phase HPLC Following Thiolysis for Quantitative Estimation and Characterization of the Four Main Classes of Phenolic Compounds in Different TIssue Zones of a Frech Cider Apple Variety (Malus domestic Var. Kemerrien). J. Agric. Food Chem. 46: 1698-1705

Hatano, T. H. Kagawa, T. Yasahura and T. Okuda. 1998. Two New Flavonoids and Other Constituents in Licorice Root: Yheri Relatitive Astringency and Radical Scavenging Effects, Chm, Phar Bull. 36: 2090-2097.

Hu, Y., J. Xu and Q. H. Hu. 2003. Evaluation of Antioxidant Potential of Aloe vera (Aloe barbadensis Mill Extracts. Journal of Agricultural and Food Chemistry. 26: 7788-7791

Hu, Q., Y. Hu and J. Xu. 2005. Free Radical - Scavenging Activity of Aloe vera (Aloe barbadensis Miller) Extracts ny Supercritical carbon Dioxide Extraction. Food Chemistry 91:85-90

Huttler, J. A., M. Salman W. B. Statunova, N. satsangi. R. F. Williams. R. T. Streeper dan S. T. Weintraub. 1996. Antiinflammatory C-Glucosyl Chromone from Aloe barbadensis J. Nat. Prod. 59: 541-543

Joshi, S. P., 1998. Chemical Constituents and Biological Activity of Aloe barbadensis - a Review. J. Med. Aroma. Plant. Sci. 20:768-773

Jung M. Y., J. P, kim and S. Y. Kim. 1999. Methanolic Extracts of Cotis Japonica Makino Reduces Photosensitized Oxidation of Oils. Food Chem. 67: 261-268

Lee, K. Y., J. P. Weintraub and B. P. Yu. 2000. Isolation and Identification of Phenolic Antioxidant from Aloe barbadensis. Free Rad. Bio & Med. 28: 261-265

Martin, T. S., H. Kikuzaki, M. Hisamoto and N. Nakatani. 2000. Constituents of Amonum tsao-ko and Their Radical Scavenging and Antioxidant Activities. J. Am. Oil. Chem. Soc. 77: 667-673

Shahidi, F. 1997. Natural Antioxidants: An Overview. In Natural Antioxidants. Chemistry, Health Effects and Applications. AOCS Press. CHampaign. Illinois. p.1-11

Schuler, P. 1990. Natural Antioxidants Exploited Commercially. In Hudson. B. J., ed FOod Antioxidants, Amsterdam: Elsevier, p. 99

Yen, G. Y., H.W. Chen and P. D. Duh. 1998. Extraction and Identification of an Antioxidative Component from Jue Ming Zi (Cassia tore L. ) J. Agric. Food Chem. Vol 46.3

Yen G. C., P.D. Duh and D. Y. Chuang. 2000. Antioxidant Activity of Anthraquinones and Anthrone. Food. Chem. 70: 437-441



DOI: https://doi.org/10.22146/jifnp.30

Article Metrics

Abstract views : 877 | views : 942

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Food and Nutrition Progress (print ISSN 0854-6177, online ISSN 2597-9388) is published by the Indonesian Association of Food Technologist in collaboration with Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada.

Journal of Indonesian Food and Nutrition Progress have been indexed by: 

   

 

This works is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.