Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin
Keywords:
allergy, inflammation, diosgenin, purple yam, steroidal saponin
Abstract
Steroidal saponin compound in Dioscorea species is one of the crucial substance because it has some biological functions, such as immunomodulatory and anti-allergic agent. This study aims to analyze the effect of steroidal saponin of Dioscorea alata L. on CD4 T cells activation, cytokines IL-4, IL-5 and TGF-β. This study used primary lymphocyte cell which isolated from BALB/c mice spleen. Mice lymphocyte were cultured in the complete medium with presence of various concentrations (50, 25 and 12.5 µg/mL) of three isolate of steroidal saponin of D. alata for 24 h. Diosgenin was used as positive control. After 24 h of treatment, the activation of lymphocyte, IL-4 and IL-5 production was determined by Flowcytometry analysis. Data were analyzed using student t-test with p < 0.05 and p < 0.01. The results showed that the low concentration of Isolate 1 (12.5 µg/mL) was found to be effective to stimulate the activation of CD4 T cells, reduce the production of IL4 and IL-5 by CD4 T cells and increase TGF-β production. These results showed that a low concentration of steroidal saponin of D. alata could be considered as immunomodulatory and anti-allergic agent. Purification and identification of each isolate isolated from DAE is in progress.
References
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Christina, Y. I., & Rifa’i, M. (2014). Bioactivity of Purple Yam Tuber (Dioscorea alata L.) on the Level of CD8+ and CD8+CD462L+ T cells and Histology of Liver in BALB/c Mice Model of Digestive Allergy. J.Exp. Life Sci, 4(2), 27–33.
D, H. C., K, R., & Dsouza, M. R. (2019). Diosgenin From Dioscorea Alata: Extraction and Potential Effects on Enzymes Related to Metabolic Syndrome. International Journal of Pharmacy and Biological Sciences, 9(1), 177–185.
Diding, Listyaningsih, E., & Subijanto, A. (2008). Pengaruh Probiotik Terhadap Gambaran Histologis Mukosa Usus Pada Mencit Balb/C Model Alergi. YARSI Medical Journal, 16(1), 6–12. https://doi.org/10.33476/JKY.V16I1.219
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Gour, N., & Wills-Karp, M. (2015). IL-4 and IL-13 signaling in allergic airway disease. Cytokine, 75(1), 68–78. https://doi.org/10.1016/J.CYTO.2015.05.014
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Jantan, I., Ahmad, W., & Bukhari, S. N. A. (2015). Plant-derived immunomodulators: An insight on their preclinical evaluation and clinical trials. Frontiers in Plant Science, 6(AUG), 655. https://doi.org/10.3389/FPLS.2015.00655/BIBTEX
Jayachandran, K. S., Rachel Vasanthi, A. H., & Gurusamy, N. (2016). Steroidal Saponin Diosgenin from Dioscorea bulbifera Protects Cardiac Cells from Hypoxia-reoxygenation Injury Through Modulation of Pro-survival and Pro-death Molecules. Pharmacognosy Magazine, 12(Suppl 1), S14–S20. https://doi.org/10.4103/0973-1296.176114
Jesus, M., Martins, A. P. J., Gallardo, E., & Silvestre, S. (2016). Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology. Journal of Analytical Methods in Chemistry, 2016. https://doi.org/10.1155/2016/4156293
Kaur, B., Khatun, S., & Suttee, A. (2021). Current Highlights on Biochemical and Pharmacological Profile of Dioscorea Alata: A Review. Plant Archieves, 21(1), 552–559. https://doi.org/10.51470/PLANTARCHIVES.2021.v21.S1.085
Koyasu, S., & Moro, K. (2011). Type 2 innate immune responses and the natural helper cell. Immunology, 132(4), 475. https://doi.org/10.1111/J.1365-2567.2011.03413.X
Lin, S., Wang, D., Yang, D., Yao, J., Tong, Y., & Chen, J. (2007). Characterization of steroidal saponins in crude extract from Dioscorea nipponica Makino by liquid chromatography tandem multi-stage mass spectrometry. Analytica Chimica Acta, 599(1), 98–106. https://doi.org/10.1016/J.ACA.2007.07.070
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Magni, P., Ruscica, M., Dozio, E., Passafaro, L., Steffani, L., Morelli, P., Banfi, G., & Corsi, M. M. (2010). Plasma adiponectin and leptin concentrations in professional rugby players. J Biol Regul Homeost Agents, 24(1), 87–91.
Makiyah, S. N. N. (2018). Steroidal Saponin Isolates of Dioscorea alata L. Induced B220IgE and B220IgG1 In Vitro. 8th Annual Basic Science International Conference Convergence of Basic Sciences, Toward the World’s Sustainability Challenges.
Makiyah, S. N. N., Djati, M. S., Muhaimin, R., & Widodo. (2015). Lymphocyte Proliferation on Hypersensitivity of Balb/C Mice After Given Ethanol Extract Tuber of Dioscorea Alata L. The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013), 2(1), 107. https://doi.org/10.18502/KLS.V2I1.125
Makiyah, S. N. N., Noor, Z., Widodo, Rifa’i, M., & Djati, M. S. (2014). Ethanol Extracts of Tubers Dioscorea Alata L. As Antiallergic Agent on Mice Balb/C Induced with Ovalbumin. International Journal of Pharma and Bio Sciences, 5(3), 214–220.
Makiyah, S. N. N., Widodo, Rifa’i, M., & Djati, M. S. (2016). The influence of ethanol extract Dioscorea alata L. on CD4+CD62L+ and CD8+CD62L+ profile of BALB/c mice model digestive tract allergy. AIP Conference Proceedings, 1744(1), 020054. https://doi.org/10.1063/1.4953528
Marwa, K., & Kondamudi, N. P. (2021). Type IV Hypersensitivity Reaction. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK562228/
Robinson, D. S., Bentley, A. M., Hartnell, A., Kay, A. B., & Durham, S. R. (1993). Activated memory T helper cells in bronchoalveolar lavage fluid from patients with atopic asthma: relation to asthma symptoms, lung function, and bronchial responsiveness. Thorax, 48(1), 26–32. https://doi.org/10.1136/THX.48.1.26
Robinson, Douglas S., Hamid, Q., Ying, S., Tsicopoulos, A., Barkans, J., Bentley, A. M., Corrigan, C., Durham, S. R., & Kay, A. B. (1992). Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma. The New England Journal of Medicine, 326(5), 298–304. https://doi.org/10.1056/NEJM199201303260504
Salehi, B., Sener, B., Kilic, M., Sharifi-Rad, J., Naz, R., Yousaf, Z., Mudau, F. N., Fokou, P. V. T., Ezzat, S. M., El Bishbishy, M. H., Taheri, Y., Lucariello, G., Durazzo, A., Lucarini, M., Suleria, H. A. R., & Santini, A. (2019). Dioscorea Plants: A Genus Rich in Vital Nutra-pharmaceuticals-A Review. Iranian Journal of Pharmaceutical Research : IJPR, 18(Suppl1), 68. https://doi.org/10.22037/IJPR.2019.112501.13795
Saxena, R., Sharma, A., Bharti, M., & Rathore, M. (2012). Immunomodulator A New Horizon: An overview. Journal of Pharmacy Research, 5, 2306–2310.
Sharma, P., Kumar, P., Sharma, R., Gupta, G., & Chaudhary, A. (2017). Immunomodulators: Role of medicinal plants in immune system. National Journal of Physiology, Pharmacy and Pharmacology, 552(6). https://doi.org/10.5455/njppp.2017.7.0203808032017
Shivhare, P., Shankarnarayan, L., Singh, A., Patil, S. T., & Yadav, M. (2015). Role of Immunomodulators in Oral Diseases. International Journal of Oral Health and Medical Research, 2(3), 73–80.
Soyer, O. U., Akdis, M., & Akdis, C. A. (2011). Mechanisms of subcutaneous allergen immunotherapy. Immunology and Allergy Clinics of North America, 31(2), 175–190. https://doi.org/10.1016/J.IAC.2011.02.006
Sprent, J., & Surh, C. D. (2011). Normal T cell homeostasis: the conversion of naive cells into memory-phenotype cells. Nature Immunology 2011 12:6, 12(6), 478–484. https://doi.org/10.1038/ni.2018
Steinke, J. W., & Borish, L. (2001). Th2 cytokines and asthma — Interleukin-4: its role in the pathogenesis of asthma, and targeting it for asthma treatment with interleukin-4 receptor antagonists. Respiratory Research, 2(2), 66. https://doi.org/10.1186/RR40
Tarapdar, S., Duggal, M., Varshney, J., Bansal, V., Tyagi, S., & Gupta, A. (2020). Potential source of immunomodulators from medicinal plant products: Review. International Journal of Botany Studies, 5(4), 232–235.
Wang, T.-S., Lii, C.-K., Huang, Y.-C., Chang, J.-Y., & Yang, F.-Y. (2011). Anticlastogenic effect of aqueous extract from water yam (Dioscorea alata L.). Journal of Medicinal Plants Research, 5(26), 6192–6202. https://doi.org/10.5897/JMPR11.1126
Yuan, S., Yan, Y. C., & Lin, H. H. (2005). Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis.
Casale, T. B., & Stokes, J. R. (2008). Immunomodulators for allergic respiratory disorders. Journal of Allergy and Clinical Immunology, 121(2), 288–296. https://doi.org/10.1016/J.JACI.2007.11.040
Catanzaro, M., Corsini, E., Rosini, M., Racchi, M., & Lanni, C. (2018). Immunomodulators Inspired by Nature: A Review on Curcumin and Echinacea. Molecules, 23(11). https://doi.org/10.3390/MOLECULES23112778
Chairunnisa, S., Wartini, N. M., & Suhendra, L. (2019). Effect of Temperature and Maseration Time on Characteristics of Bidara Leaf Extract (Ziziphus mauritiana L.) as Saponin Source. Jurnal Rekayasa Dan Manajemen Agroindustri, 7(4), 551–560. https://doi.org/https://doi.org/10.24843/JRMA.2019.v07.i04.p07
Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., & Zhao, L. (2018). Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 9(6), 7204. https://doi.org/10.18632/ONCOTARGET.23208
Cheng, W. Y., Kuo, Y. H., & Huang, C. J. (2007). Isolation and identification of novel estrogenic compounds in yam tuber (Dioscorea alata Cv. Tainung No. 2). Journal of Agricultural and Food Chemistry, 55(18), 7350–7358. https://doi.org/10.1021/JF0711690
Christina, Y. I., & Rifa’i, M. (2014). Bioactivity of Purple Yam Tuber (Dioscorea alata L.) on the Level of CD8+ and CD8+CD462L+ T cells and Histology of Liver in BALB/c Mice Model of Digestive Allergy. J.Exp. Life Sci, 4(2), 27–33.
D, H. C., K, R., & Dsouza, M. R. (2019). Diosgenin From Dioscorea Alata: Extraction and Potential Effects on Enzymes Related to Metabolic Syndrome. International Journal of Pharmacy and Biological Sciences, 9(1), 177–185.
Diding, Listyaningsih, E., & Subijanto, A. (2008). Pengaruh Probiotik Terhadap Gambaran Histologis Mukosa Usus Pada Mencit Balb/C Model Alergi. YARSI Medical Journal, 16(1), 6–12. https://doi.org/10.33476/JKY.V16I1.219
Dong, Y. K., Hye, Y. M., CHIN, Y.-W., Park, J. ., & JINWOONG, K. (2008). Determination of Allantoin in Dioscorea Rhizoma by High Performance Liquid Chromatography Using Cyano Columns. Natural Product Sciences, 14(4), 254–259.
Fischer, R., McGhee, J. R., Vu, H. L., Atkinson, T. P., Jackson, R. J., Tomé, D., & Boyaka, P. N. (2005). Oral and nasal sensitization promote distinct immune responses and lung reactivity in a mouse model of peanut allergy. The American Journal of Pathology, 167(6), 1621–1630. https://doi.org/10.1016/S0002-9440(10)61246-1
Georas, S. ., Guo, J., Fanis, U. De, & Casolaro, V. (2005). Series Signalling and Transcriptional Regulation in Inflammatory and Immune Cells: Importance in Lung Biology and Disease. European Respiratory Journal, 26, 1119–1137.
Gour, N., & Wills-Karp, M. (2015). IL-4 and IL-13 signaling in allergic airway disease. Cytokine, 75(1), 68–78. https://doi.org/10.1016/J.CYTO.2015.05.014
Greenfeder, S., Umland, S. P., Cuss, F. M., Chapman, R. W., & Egan, R. W. (2001). Th2 cytokines and asthma. The role of interleukin-5 in allergic eosinophilic disease. Respiratory Research, 2(2), 71–79. https://doi.org/10.1186/RR41/METRICS
Harun, N. H., Septama, A. W., Ahmad, W. A. N. W., & Suppian, R. (2020). Immunomodulatory effects and structure-activity relationship of botanical pentacyclic triterpenes: A review. Chinese Herbal Medicines, 12(2), 118–124. https://doi.org/10.1016/J.CHMED.2019.11.007
Huber, S., Gagliani, N., O’Connor, W., Geginat, J., & Caprioli, F. (2017). CD4 + T Helper Cell Plasticity in Infection, Inflammation, and Autoimmunity. Mediators of Inflammation, 2017. https://doi.org/10.1155/2017/7083153
Jacobsen, E. A., Taranova, A. G., Lee, N. A., & Lee, J. J. (2007). Eosinophils: singularly destructive effector cells or purveyors of immunoregulation? The Journal of Allergy and Clinical Immunology, 119(6), 1313–1320. https://doi.org/10.1016/J.JACI.2007.03.043
Jantan, I., Ahmad, W., & Bukhari, S. N. A. (2015). Plant-derived immunomodulators: An insight on their preclinical evaluation and clinical trials. Frontiers in Plant Science, 6(AUG), 655. https://doi.org/10.3389/FPLS.2015.00655/BIBTEX
Jayachandran, K. S., Rachel Vasanthi, A. H., & Gurusamy, N. (2016). Steroidal Saponin Diosgenin from Dioscorea bulbifera Protects Cardiac Cells from Hypoxia-reoxygenation Injury Through Modulation of Pro-survival and Pro-death Molecules. Pharmacognosy Magazine, 12(Suppl 1), S14–S20. https://doi.org/10.4103/0973-1296.176114
Jesus, M., Martins, A. P. J., Gallardo, E., & Silvestre, S. (2016). Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology. Journal of Analytical Methods in Chemistry, 2016. https://doi.org/10.1155/2016/4156293
Kaur, B., Khatun, S., & Suttee, A. (2021). Current Highlights on Biochemical and Pharmacological Profile of Dioscorea Alata: A Review. Plant Archieves, 21(1), 552–559. https://doi.org/10.51470/PLANTARCHIVES.2021.v21.S1.085
Koyasu, S., & Moro, K. (2011). Type 2 innate immune responses and the natural helper cell. Immunology, 132(4), 475. https://doi.org/10.1111/J.1365-2567.2011.03413.X
Lin, S., Wang, D., Yang, D., Yao, J., Tong, Y., & Chen, J. (2007). Characterization of steroidal saponins in crude extract from Dioscorea nipponica Makino by liquid chromatography tandem multi-stage mass spectrometry. Analytica Chimica Acta, 599(1), 98–106. https://doi.org/10.1016/J.ACA.2007.07.070
Luis Muñoz-Carrillo, J., Castro-García, F. P., Chávez-Rubalcaba, F., Chávez-Rubalcaba, I., Luis Martínez-Rodríguez, J., Elizabeth Hernández-Ruiz, M., Chávez-Ruvalcaba, F., & Chávez-Ruvalcaba, I. (2018). Immune System Disorders: Hypersensitivity and Autoimmunity. Immunoregulatory Aspects of Immunotherapy. https://doi.org/10.5772/INTECHOPEN.75794
Macey, M. R., Sturgill, J. L., Morales, J. K., Falanga, Y. T., Morales, J., Norton, S. K., Yerram, N., Shim, H., Fernando, J., Gifillan, A. M., Gomez, G., Schwartz, L., Oskeritzian, C., Spiegel, S., Conrad, D., & Ryan, J. J. (2010). IL-4 and TGF-β1 Counterbalance One Another while Regulating Mast Cell Homeostasis. The Journal of Immunology, 184(9), 4688–4695. https://doi.org/10.4049/JIMMUNOL.0903477
Magni, P., Ruscica, M., Dozio, E., Passafaro, L., Steffani, L., Morelli, P., Banfi, G., & Corsi, M. M. (2010). Plasma adiponectin and leptin concentrations in professional rugby players. J Biol Regul Homeost Agents, 24(1), 87–91.
Makiyah, S. N. N. (2018). Steroidal Saponin Isolates of Dioscorea alata L. Induced B220IgE and B220IgG1 In Vitro. 8th Annual Basic Science International Conference Convergence of Basic Sciences, Toward the World’s Sustainability Challenges.
Makiyah, S. N. N., Djati, M. S., Muhaimin, R., & Widodo. (2015). Lymphocyte Proliferation on Hypersensitivity of Balb/C Mice After Given Ethanol Extract Tuber of Dioscorea Alata L. The 3rd International Conference on Biological Science 2013 (The 3rd ICBS-2013), 2(1), 107. https://doi.org/10.18502/KLS.V2I1.125
Makiyah, S. N. N., Noor, Z., Widodo, Rifa’i, M., & Djati, M. S. (2014). Ethanol Extracts of Tubers Dioscorea Alata L. As Antiallergic Agent on Mice Balb/C Induced with Ovalbumin. International Journal of Pharma and Bio Sciences, 5(3), 214–220.
Makiyah, S. N. N., Widodo, Rifa’i, M., & Djati, M. S. (2016). The influence of ethanol extract Dioscorea alata L. on CD4+CD62L+ and CD8+CD62L+ profile of BALB/c mice model digestive tract allergy. AIP Conference Proceedings, 1744(1), 020054. https://doi.org/10.1063/1.4953528
Marwa, K., & Kondamudi, N. P. (2021). Type IV Hypersensitivity Reaction. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK562228/
Robinson, D. S., Bentley, A. M., Hartnell, A., Kay, A. B., & Durham, S. R. (1993). Activated memory T helper cells in bronchoalveolar lavage fluid from patients with atopic asthma: relation to asthma symptoms, lung function, and bronchial responsiveness. Thorax, 48(1), 26–32. https://doi.org/10.1136/THX.48.1.26
Robinson, Douglas S., Hamid, Q., Ying, S., Tsicopoulos, A., Barkans, J., Bentley, A. M., Corrigan, C., Durham, S. R., & Kay, A. B. (1992). Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma. The New England Journal of Medicine, 326(5), 298–304. https://doi.org/10.1056/NEJM199201303260504
Salehi, B., Sener, B., Kilic, M., Sharifi-Rad, J., Naz, R., Yousaf, Z., Mudau, F. N., Fokou, P. V. T., Ezzat, S. M., El Bishbishy, M. H., Taheri, Y., Lucariello, G., Durazzo, A., Lucarini, M., Suleria, H. A. R., & Santini, A. (2019). Dioscorea Plants: A Genus Rich in Vital Nutra-pharmaceuticals-A Review. Iranian Journal of Pharmaceutical Research : IJPR, 18(Suppl1), 68. https://doi.org/10.22037/IJPR.2019.112501.13795
Saxena, R., Sharma, A., Bharti, M., & Rathore, M. (2012). Immunomodulator A New Horizon: An overview. Journal of Pharmacy Research, 5, 2306–2310.
Sharma, P., Kumar, P., Sharma, R., Gupta, G., & Chaudhary, A. (2017). Immunomodulators: Role of medicinal plants in immune system. National Journal of Physiology, Pharmacy and Pharmacology, 552(6). https://doi.org/10.5455/njppp.2017.7.0203808032017
Shivhare, P., Shankarnarayan, L., Singh, A., Patil, S. T., & Yadav, M. (2015). Role of Immunomodulators in Oral Diseases. International Journal of Oral Health and Medical Research, 2(3), 73–80.
Soyer, O. U., Akdis, M., & Akdis, C. A. (2011). Mechanisms of subcutaneous allergen immunotherapy. Immunology and Allergy Clinics of North America, 31(2), 175–190. https://doi.org/10.1016/J.IAC.2011.02.006
Sprent, J., & Surh, C. D. (2011). Normal T cell homeostasis: the conversion of naive cells into memory-phenotype cells. Nature Immunology 2011 12:6, 12(6), 478–484. https://doi.org/10.1038/ni.2018
Steinke, J. W., & Borish, L. (2001). Th2 cytokines and asthma — Interleukin-4: its role in the pathogenesis of asthma, and targeting it for asthma treatment with interleukin-4 receptor antagonists. Respiratory Research, 2(2), 66. https://doi.org/10.1186/RR40
Tarapdar, S., Duggal, M., Varshney, J., Bansal, V., Tyagi, S., & Gupta, A. (2020). Potential source of immunomodulators from medicinal plant products: Review. International Journal of Botany Studies, 5(4), 232–235.
Wang, T.-S., Lii, C.-K., Huang, Y.-C., Chang, J.-Y., & Yang, F.-Y. (2011). Anticlastogenic effect of aqueous extract from water yam (Dioscorea alata L.). Journal of Medicinal Plants Research, 5(26), 6192–6202. https://doi.org/10.5897/JMPR11.1126
Yuan, S., Yan, Y. C., & Lin, H. H. (2005). Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis.
Published
2023-03-31
How to Cite
Makiyah, S. N. N., Tasminatun, S., Djati, M. S., Rifa’i, M., & Widodo, W. (2023). Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin. Indonesian Journal of Pharmacy, 34(1), 153-161. https://doi.org/10.22146/ijp.4614
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Research Article
