Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples

  • Kusnul Khotimah Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia
  • Sudibyo Martono Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia; The National Agency of Drug and Food Control, District of Yogyakarta, Republic of Indonesia
  • Anjar Windarsih Research Division for Natural Product Technology (BPTBA), Indonesian Institute of Sciences (LIPI), Yogyakarta 55861, Indonesia.
  • Irnawati Irnawati Faculty of Pharmacy, Halu Oleo University, Kendari 93232, Indonesia
  • Erna Prihandiwati School of Health Sciences ISFI Banjarmasin, South Kalimantan, Indonesia, 70123
  • Abdul Rohman Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia; Center of Excellence (CoE), Institute of Halal Industry and Systems (IHIS), Gadjah Mada University, Yogyakarta, 55281 Indonesia
Keywords: chloramphenicol, hydrocortisone acetate, FTIR spectroscopy, partial least square, cream

Abstract

Analysis of chloramphenicol (CL) and hydrocortisone acetate (HCA) in cream samples using FTIR (Fourier Transform Infrared) spectroscopy. The objective of this study was to develop FTIR spectroscopy combined with multivariate calibrations for effective analysis of CL and HCA in cream formulation. High performance liquid chromatography (HPLC) method was applied for determining the actual value of CL and HCA. Cream samples containing CL and HCA were scanned using FTIR spectrophotometer employing attenuated total reflectance (ATR) technique. FTIR-ATR spectra were subjected to several optimization including wavenumbers selection and derivatization for quantitative analysis. The results showed that the optimum prediction model for correlation between actual values of CL and HCA as determined by HPLC and FTIR-predicted values was obtained using first derivative FTIR spectra at wavenumbers of 1500-1000 cm-1. The R2 value for calibration and internal validation for CL and HCA was > 0.9 with relatively small RMSEC (root mean square error of calibrations), RMSECV (root mean square error of cross validations) and PRESS (predicted residual error sum of squares) values. External validation of CL and HCA models produced R2 > 0.98 with RMSEP values of 0.6501 and 1.0041, respectively. The results of CL and HCA assay using HPLC and FTIR spectroscopy were compared resulting non-significant results (p > 0.05) for these two methods. FTIR spectroscopy in combination with PLS can be used as a rapid and reliable analytical method for determination of CL and HCA in cream formulation.

Author Biographies

Sudibyo Martono, Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia; The National Agency of Drug and Food Control, District of Yogyakarta, Republic of Indonesia

Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia

The National Agency of Drug and Food Control, District of Yogyakarta, Republic of Indonesia

Abdul Rohman, Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia; Center of Excellence (CoE), Institute of Halal Industry and Systems (IHIS), Gadjah Mada University, Yogyakarta, 55281 Indonesia

Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia; Center of Excellence (CoE), Institute of Halal Industry and Systems (IHIS), Gadjah Mada University, Yogyakarta, 55281 Indonesia

References

Abbas N., Arshad MS., Hussain A., Irfan M., Ahsan M., Rasool MF., 2017. Development and validation of a spectroscopic method for the simultaneous analysis of miconazole nitrate and hydrocortisone acetate in pharmaceutical dosage form. Trop. J. Pharm. Res. 16: 413-18.
Altamimi MA., Elzayat EM., Qamar W., Alshehri SM., Sherif AY., Haq N., Shakeel F., 2019. Evaluation of the bioavailability of hydrocortisone when prepared as solid dispersion. Saudi Pharm. J. 27: 629-36.
Brayfield A., Cadart CR., Crehan EE., Eager K. 2005. Martindale the complete drug reference. 38th ed. Pharmaceutical Press, London. pp 169-70.
Guntarty A., Ahda M., Kusbandari A., Prihandoko SW., 2019. Analysis of lard in sausage using Fourier transform infrared spectrophotometer combined with chemometrics. J. Pharm. Bioallied. Sci. 11: 594-600.
Iqbal M., Shad MW., Ashraf M., Bilal M., Saeed M., 2006. Development and validation of an HPLC method for the determination of dexamethasone, dexamethasone sodium phosphate and chloramphenicol in presence of each other in pharmaceutical preparations. Chromatographia. 64: 219–22.
Karoui R., Downey G., Blecker C., 2010. Mid-infrared spectroscopy coupled with chemometrics: a tool for the analysis of intact food systems and the exploration of their molecular structure−quality relationships − a review. Chem. Rev.110: 6144–68.
Karthikeyan S., 2011. UV-Visible and infrared analysis of commercial drug and its mixtures. Arch. Physics. Res. 2: 72-9.
Katakam P., 2012. Stability indicating HPLC method for simultaneous determination of chloramphenicol and prednisolone acetate in bulk and formulations. Int. J. Pharm. Pharm. Sci. 5: 182–5.

Khajehsharifi H., Pourbasheer E., Tavallali H., Sarvi S., Sadeghi M., 2014. The comparison of partial least squares and principal component regression in simultaneous spectrophotometric determination of ascorbic acid, dopamine and uric acid in real samples. Arab. J. Chem. 10: S3451-58.
Kristiningrum N., Rakhmawati M., 2021, Simultaneous determination of chloramphenicol and hydrocortisone acetate in cream using TLC densitometry method. Int. Curr. Pharm. 2: 7–10.
Livingston RJ., Butterworth JW., Belt P., 2013. Reaction or infection: topical chloramphenicol treatment. Ann. R Coll. Surg. Engl. 95: e20–1.
Miller JN., Miller JC. 2010. Statistic and chemometrics for analytical chemistry, 6th ed. Prentice Hall, England. Pp. 237-45.
Moffat AC., Watts J., Clarke EGC. 2011. Clarke’s analysis of drugs and poisons: in pharmaceuticals, body fluids and postmortem material. 4th ed. Pharmaceutical Press, London.
Pavia DL., Lampman GM., Kriz GS., Vyvyan JR., 2009. Introduction to spectroscopy, 4th ed. W.B. Saunders Company, Philadelpia.
Pebriana RB., Rohman A., Lukitaningsih E., Sudjadi, 2017. Development of FTIR spectroscopy in combination with chemometrics for analysis of rat meat in beef sausage employing three lipid extraction systems. Int. J. Food Prop. 20: S1995-S2005.
Prabaningdyah NK., Riyanto S., Rohman A., 2018. Application of FTIR spectroscopy and multivariate calibration for analysis of curcuminoid in syrup formulation. J Applied Pharm. Sci. 8: 172-79.
Roggo Y., Chalus P., Maurer L., Lema-Martinez C., Edmond A., Jent N., 2007. A review of near infrared spectroscopy and chemometrics in pharmaceutical technologies. J. Pharm. Biomed. Anal. 44: 683–700.
Rohman A., Arsanti L., Erwanto Y., Pranoto Y., 2016. The use of vibrational spectroscopy and chemometrics in the analysis of pig derivatives for halal authentication. IFRJ. 23: 1839–48.
Rohman A., Windarsih A., Hossain MAM., Johan MR., Ali ME., Fadzillah NA., 2019. Application of near- and mid-infrared spectroscopy combined with chemometrics for discrimination and authentication of herbal products: A review. J. Applied Pharm. Sci. 9: 137-47.
Shadoul WA., Kariem EAG., Adam ME., Ibrahim, KEE., 2011. Simultaneous determination of dexamethasone sodium phosphate and chloramphenicol in ophthalmic solutions. Int. J. Chem. Sci. Technol. 1: 60–9.
Worley B., Powers R., 2013. Multivariate analysis in metabolomics. Curr. Metabolomics. 1: 92-107.
Xiaoyan L., 1998. Determination of two components in chloramphenicol and hydrocortisone ear drops by high performance liquid chromatography. Chinese. J. Chromato. 16: 71–3.
Published
2021-09-29
How to Cite
Khotimah, K., Martono, S., Windarsih, A., Irnawati, I., Prihandiwati, E., & Rohman, A. (2021). Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples. Indonesian Journal of Pharmacy, 32(3), 408-415. Retrieved from https://dev.journal.ugm.ac.id/v3/IJP/article/view/2687
Section
Research Article