* Corresponding Author
Efek Pemberian Ekstrak Lidah Buaya (Aloe Barbadensis Miller) pada Soket Gigi terhadap Kepadatan Serabut Kolagen Pasca Ekstraksi Gigi Marmut (Cavia Porcellus)
https://doi.org/10.22146/majkedgiind.8743 Fatma Yuza(1*), Ivan Arie Wahyudi(2), Sri Larnani(3)
(1) Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(2) Bagian Biomedika, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Bagian Biomedika, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Tindakan ekstraksi gigi menyebabkan terjadinya luka sehingga akan melibatkan proses penyembuhan luka pada jaringan. Salah satu tahap penting dari proses penyembuhan luka pasca esktraksi gigi adalah terbentuknya serabut kolagen. Lidah buaya (Aloe barbadensis Miller) mengandung saponin, vitamin C dan acemannan yang diduga membantu
proses pembentukan serabut kolagen. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak lidah buaya terhadap kepadatan serabut kolagen pada proses penyembuhan luka pasca ekstraksi gigi marmut (Cavia porcellus). Lidah buaya yang digunakan berasal dari Sleman, Yogyakarta. Pembuatan ekstrak menggunakan metode maserasi dan pelarut air. Selanjutnya, dua puluh tujuh ekor marmut dibagi ke dalam kelompok kontrol dan kelompok perlakuan. Kelompok perlakuan terdiri dari kelompok ekstrak lidah buaya 45% dan 90%. Ekstrak lidah buaya sebanyak 0,05ml diteteskan kedalam soket gigi marmut pasca ekstraksi gigi pada kelompok perlakuan. Soket gigi marmut kelompok kontrol tidak diberi aplikasi zat aktif apapun. Tiga ekor subjek dari masing-masing kelompok dikorbankan pada hari ke-3, 7, dan 14 setelah ekstraksi gigi. Preparat histologis kepadatan kolagen soket gigi marmut diamati dengan menggunakan mikroskop cahaya perbesaran 400x. Analisis data dilakukan dengan menggunakan uji Kruskal-Wallis dan dilanjutkan
dengan uji Post Hoc menggunakan uji Mann-Whitney untuk membandingkan kepadatan kolagen antar kelompok pasca ekstraksi gigi marmut. Hasil uji statistik antar kelompok menunjukkan bahwa ekstrak lidah buaya 90% berpengaruh pada pembentukan serabut kolagen jika dibandingkan dengan kelompok kontrol (p<0,05) pada hari ke-7 pasca ekstraksi gigi marmut. Kesimpulan dari penelitian ini adalah ekstrak lidah buaya 90% dapat membantu meningkatkan kepadatan serabut kolagen soket gigi hari ke-7 pasca ekstraksi gigi marmut.
The Effect of Aloe Barbadensis Miller Extract to The Density of Collagen Fibers in The Wound Healing Process after Tooth Extraction of Guinea Pig (Cavia porcellus). Tooth extraction causes wound that would involve wound healing process on tissue. One of the important stages of wound healing process after dental extraction is the formation of collagen fibers. Aloe barbadensis Miller contains saponins, vitamin C and ace mannan that allegedly assist the process of collagen fibers formation. The purpose of this study was to determine the effect of Aloe barbadensis Miller extract to the density of collagen fibers in the wound healing process after tooth extraction of guinea pig (Cavia
porcellus). Aloe vera is used in this study came from Sleman, Yogyakarta. Extract is made with maceration method and water as the solvent. Furthermore, twenty-seven guinea pigs were divided into a control group and treatment groups. The treatment groups consisted 45% Aloe barbadensis Miller extract group and 90% Aloe barbadensis Miller extract
group. Aloe barbadensis Miller extract as much as 0.05 ml dropped into guinea pigs tooth sockets after tooth extraction in the treatment groups. Guinea pig’s tooth socket of the control group was not given any active substance. Three guinea pigs of each group were sacrificed on day 3, 7, and 14 after tooth extraction. Histology preparations of guinea pig teeth
sockets density of collagen were observed using light microscope 400x magnification. Analyzing data is done by Kruskal-Wallis test followed by Post Hoc test using the Mann-Whitney test for comparing collagen density between groups. Statistically results between groups showed that the extract of 90% Aloe barbadensis Miller affected the formation of collagen fibers when compared to the control group (p <0.05) on day 7 after tooth extraction of guinea pig. The conclusion of this study was 90% Aloe barbadensis Miller extract increased the density of collagen fibers from the tooth socket seven days after tooth extraction of guinea pig.
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Balaji SM. Text Book of Oral and Maxillofacial Surgery. New Delhi: Elsevier; 2007. p. 211. Diakses dari http://books.google.co.id/books?Balaji.,+2007,+Textbook+of+Oral+and+Maxi
llofacial+Surgery diunduh 4 Februari 2013.
Gottrup F, Jensen SS, Andreasen JO. Wound Healing Subsequent to Injury. In: Andreasen JO, Andreasen FM, Andersson L, eds, Textbook and Color Atlas of Traumatic
Injuries to the Teeth. 4th ed. Oxford: Blackwell Publishing Ltd.; 2007. p. 1,4, 6, 23, 26, 32, 41.
Švermová D. Teeth Extraction. In: Dostálová T and Seydlová M, eds, Dentistry and Oral Diseases. Praha: Grada Publishing;
p. 74. Diakses dari http://google.co.id/books?Švermová.+D.,+ 2010+Teeth+Extraction,+in+Dostálová.diunduh 12 Februari 2013.
Balogh MB, Fehrenbach MJ. Dental Embryology, Histology, and Anatomy. 2nd ed.Philadelphia: Elsevier Inc.; 2006. p. 112-3.
Panda H. Aloe vera Handbook Cultivation, Research Findings, Products, Formulations, Extraction and Processing. New Delhi: Asia Pacific Business Press Inc.; 2003. p. 16, 40. Diakses dari http://books.google.co.id/books?id=zqwL83POxWoCprintsec=frontcoverdq=panda+aloe+vera+handbook&hl=id&sa=X&ei=_r00UZjqNsSzrAeh-oBY&redir_esc=y.
diunduh 13 Februari 2013.
Barcroft A, Myskja,A. Aloe vera Nature’s Silent Healer. London: BAAM Publishing Ltd.;2003.
p. 1, 27, 28, 30, 33, 34, 38, 39, 46. Diakses
dari http://books.google.co.id?Barcroft+Aloe+vera
nature’s+silent+healer. diunduh 13 Februari 2013.
Nusgens BV, Humbert P, Rougier A, Colige AC, Haftek M, Lambert CA, Richard A, Creidi P, Lapière M. Topically Applied Vitamin C Enhances the mRNA Level of Collagens I and III, Their Processing Enzymes and Tissue Inhibitor of Matrix Metalloproteinase 1 in the Human Dermis. Journal of Investigative
Dermatology. 2001; 116(16): 853-859.
Moon HI, Chung JH, Lee JK, Zee OP. Tritepenoid saponin from Viola hondoensisW. Becker et H Boss. and their effect on MMP-
and type I procollagen expression. Archives Pharmacal Research. 2004; 7(27): 730-733.
Sahawat D, Khantasuwan S, Sangvanich P, Takata T, Kitagawa M, Thunyakitpisal P. Acemannan Induces Cementoblast
Proliferation, and Mineral Deposition. Journal of Medicinal Plants Research. 2012; 6(23): 4069-4076.
Arijani E, Khoswanto C. The Use of 90% Aloe vera Freeze Drying as The Modulator of Collagen Density On Extraction Socket of Incicivus Cavia cobaya. Dental Journal. 2008;
(2): 74-6.
Irshad S, Butt M, Younus H. In-Vitro Antibacterial Activity of Aloe Barbadensis Miller (Aloe Vera). International Research
Journal of Pharmaceuticals. 2011; 2(1): 59-64.
Furnawanthi I. Khasiat dan Manfaat Lidah Buaya Si
Tanaman Ajaib. Jakarta: Agromedia Pustaka; 2007. p. ,1, 3, 4, 10. Diakses dari http://books.google.co.id/bookfurnawanthi+khasiat+manfaat diunduh 13 Februari 2013.
Cho S, Lee S, Lee MJ, Lee DH, Won CH, Kim SM, Chung JH. Dietary Aloe Vera Supplementation Improves Facial Wrinkles
and Elasticity and It Increases the Type I Procollagen Gene Expression in Human Skin in vivo. Ann Dermatol (Seoul). 2009; 1(21): 6-11.
Glitz D. Protein Shyntesis: Translation and Posttranslational Modifications. In: Devlin TM, eds, Textbook of Biochemistry With Clinical Correlations. 7th ed. Philadelphia: John Wiley and Sons Inc.; 2011. p. 232, 244.
Schultz RM. In: Devlin TM, eds, Textbook of Biochemistry With Clinical Correlations. 7th ed. Philadelphia: John Wiley and Sons Inc.; 2010. p. 103.
Reis-Filho CR, Silva ER, Martins AB, Pessoa FF, Gomes PVN, Araújo MSC, Miziara MN, Alves JB. Demineralised Human Dentine
Matrix Stimulates The Expression of VEGF and Accelerates The Bone Repair In Tooth Sockets of Rats. Archieve of Oral Biology.
; 5(57): 469-479.
Yugoshi LI, Sala MA, Brentegani LG, Carvalho TLL. Histometric Study of Socket Healing after Tooth Extraction in Rats Treated with Diclofenac. Brazil Dental Journal. 2002; 13(2):
-6.
Fawcett DW. Buku Ajar Histologi (A Textbook of Histology) terj.). 2nd ed. Jakarta: EGC; 2002. p. 122-3, 125, 132, 178.
Berkovitz BKB, Moxham BJ, Linden RWA, Sloan AJ. Master Dentistry. 3th ed. London: Elsevier Ltd.; 2011. p. 221.
Trombelli L, Farina R, Marzola A, Bozzi L, Lindhe J. Modeling and Remodeling of Human Extraction Sockets. Journal of Clinical
Periodontology. 2008; 35: 630-9.
Subramanian S, Kumar DS, Arulselvan P. Wound Healing Potential of Aloe vera Leaf Gel Studied in Experimental Rabbits. Asian Journal of Biochemistry. 2008; 1(2): 178-185.
Rodwell VW. Catabolism of Proteins and of Amino Acid Nitrogen. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, eds, Harper’s Illustrated Biochemistry. 26th ed. New York: Mc
Graw-Hill Companies Inc.; 2007. p. 244.
Arunkumar S, Muthuselvam M. Analysis of Phytochemical Constituents and Antimicrobial Activities of Aloe vera L. Against Clinical Pathogens. World Journal of Agriculture
Sciences. 2009; 5(5): 572-6.
Hwang YP, Kim HG, Choi JH, Han EH, Kwon K, Lee YC, Choi JM, Chung YC, Jeon TC, Jeong HG. Saponin From The Roots of Platycodon grandiflorum Supress Ultraviolet A-Induced Matrix Metalloproteinase-1 Expression Via MAPKs and NF-κB/Ap-1-Dependent Signaling in HaCaT Cells. Food and Chemical
Toxicology. 2011; 12(49): 3374-82.
Hoffmann D. Medical Herbalism : The Science and Practice of Herbal Medicine. New York: Healing Arts Press; 2003. p. 74-5. Diakses dari http://google.co.id/books?hoffman+medical+
herbalism. diunduh 13 Februari 2013.
Mayes PA, Bender DA. Carbohydrates of Physiologic Significance. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, eds, Harper’s Illustrated Biochemistry. 26th ed. New
York: Mc Graw-Hill Companies Inc.; 2003. p. 105.
Murray RK, Keeley FW. The Extracellular Matrix. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, eds, Harper’s Illustrated
Biochemistry. 26th ed. New York: Mc Graw-Hill Companies Inc.;2003. p. 535, 537.
Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS, Lee CK. Acemannan purified from Aloe vera induces phentpic and functional maturation of immature dendritic cells. International Immunopharmacology. 2001; (1): 1275-84.
Garg HG, Longaker MT. Scarless Wound Healing. New York: Marcel Dekker, Inc.; 2005. p. 219.
DOI: https://doi.org/10.22146/majkedgiind.8743
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