Vitamin D inhibits TNF-α serum level in wistar rats stimulated with Porphyromonas gingivalis

https://doi.org/10.22146/majkedgiind.81563

Hervina Hervina(1*), Dwis Syahriel(2), Dewa Made Wedagama(3), Dewi Farida Nurlitasari(4), Haris Nasutianto(5), I Gusti Ayu Dewi Haryani(6)

(1) Department of Periodontology, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali
(2) Department of Periodontology, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali
(3) Department of Conservative Dentistry, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali
(4) Department of Prosthodontic, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali
(5) Department of Dental Maxillofacial Radiology, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali
(6) Department of Periodontology, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali
(*) Corresponding Author

Abstract


Periodontitis is a chronic inflammation of the periodontal tissues initiated by increased proinflammatory cytokines such as TNF-α due to the induction of Porphyromonas gingivalis (Pg). This study aimed to analyze the effect of vitamin D (cholecalciferol) on TNF-α serum level in Wistar rats stimulated with Pg to induce periodontitis. Twenty-seven male Wistar rats (n = 27) were divided into three equal groups. Group I was healthy Wistar rats that received 2000 IU vitamin D once a day. Group II was Wistar rats stimulated with Pg and received 2000 IU vitamin D, while group III was Wistar rats stimulated with Pg but did not receive vitamin D. Blood was collected through the orbital sinus and centrifuged to get the serum. TNF-α serum levels were assessed using Elisa method on the 7th, 14th, and 28th. The data were normally distributed and homogeneous. The mean TNF-α data was analyzed for differences between groups using the one-way ANOVA and LSD post hoc test. Significant differences were seen in group II (418.49 ± 161.08 ng/mL) and group III (172.16 ± 104.18 ng/mL) on the 28th day (p = 0.001). The findings suggest that vitamin D inhibits the TNF-α serum level in Wistar rats stimulated with Pg on the 28th day.

Keywords


periodontitis; Porphyromonas gingivalis; TNF-α; Vitamin D

Full Text:

4. Hervina


References

1. Eke PI, Dye BA, Wei L, Slade GD, Thornton-Evans GO, Borgnakke WS, et al. Update on
Prevalence of Periodontitis in Adults in the United States: NHANES 2009 to 2012. J Periodontol. 2015; 86(5): 611–22. doi: 10.1902/jop.2015.140520

2. Kementerian Kesehatan Republik Indonesia. Laporan Nasional Riset Kesehatan Dasar.
Kementeri Kesehat RI. 2018;1–582.

3. Popova C, Dosseva-Panova V, Panov V. Microbiology of periodontal diseases. A review.
Biotechnol Biotechnol Equip. 2013; 27(3): 3754–3759. doi: 10.5504/BBEQ.2013.0027

4. Patini R, Staderini E, Lajolo C, Lopetuso L, Mohammed H, Rimondini L, et al. Relationship
between oral microbiota and periodontal disease: A systematic review. Eur Rev Med Pharmacol Sci. 2018; 22(18): 5775–5788. doi: 10.26355/eurrev_201809_15903

5. Kato Y, Hagiwara M, Ishihara Y, Isoda R, Sugiura S, Komatsu T, et al. TNF-α augmented
Porphyromonas gingivalis invasion in human gingival epithelial cells through Rab5 and
ICAM-1. BMC Microbiol. 2014; 14(1): 1–13. doi: 10.1186/s12866-014-0229-z

6. Tawfig N. Proinflammatory cytokines and periodontal disease. J Dent Probl Solut. 2016; 3: 012–017. doi: 10.17352/2394-8418.000026

7. Price J, Aditi V, Rajat D, Neha S, Anuj Singh P, Rochira M. Comparative evaluation of serum
tumor necrosis factor alpha in health and chronic periodontitis: a case-control study. Contemp Clin Dent. 2020; 11(4): 342–349.

8. Meghil MM, Cutler CW. Influence of Vitamin D on periodontal inflammation: a review.
pathogens. 2023; 12(9): 1180. doi: 10.3390/pathogens12091180

9. Anand N, Chandrasekaran SC, Rajput NS. Vitamin D and periodontal health: Current
concepts. J Indian Soc Periodontol. 2013; 17(3): 302–308. doi: 10.4103/0972-124X.115645

10. Alshouibi EN, Kaye EK, Cabral HJ, Leone CW, Garcia RI. Vitamin D and periodontal
health in older men. J Dent Res. 2013; 92(8): 689–693. doi: 10.1177/0022034513495239

11. Hiremath VP, Rao CB, Naiak V, Prasad KV. Anti-inflammatory effect of vitamin D on gingivitis: a dose response randomised controlled trial. Indian J Public Health. 2013;
57(1): 29–32. doi: 10.4103/0019-557X.111365

12. Ramasamy I. Vitamin D metabolism and guidelines for Vitamin D supplementation.
Clin Biochem Rev. 2020; 41(3): 103–126. doi: 10.33176/AACB-20-00006

13. Varghese SS, Thomas H, Jayakumar ND, Sankari M, Lakshmanan R. Estimation
of salivary tumor necrosis factor-alpha in chronic and aggressive periodontitis patients.
Contemp Clin Dent. 2015; 6: S152–156. doi: 10.4103/0976-237X.166816

14. Tomar N, Gupta C, Kaushik M, Wadhawan A. Nutrigenomics: A perio-nutrition
interrelationship. J Oral Res Rev. 2017; 9(1): 32. doi: 10.4103/2249-4987.201401

15. Noh MK, Jung M, Kim SH, Lee SR, Park KH, Kim DH, et al. Assessment of IL-6, IL-8 and
TNF-α levels in the gingival tissue of patients with periodontitis. Exp Ther Med. 2013; 6(3):
847–851. doi: 10.3892/etm.2013.1222

16. Rathinasamy K, Ulaganathan A, Ramamurthy S, Ganesan R, Saket P, Alamelu S. Estimation
of TNF-α levels in saliva and serum of patients with periodontal health and chronic
periodontitis: A case-control study. J Contemp Dent Pract. 2020; 21(2): 148–151.
doi: 10.5005/jp-journals-10024-2765

17. Luo G, Li F, Li X, Wang ZG, Zhang B. TNF-α and RANKL promote osteoclastogenesis by
upregulating RANK via the NF-κB pathway. Mol Med Rep. 2018; 17(5): 6605–6611.
doi: 10.3892/mmr.2018.8698

18. Yuce HB, Gokturk O, Turkal HA, Inanir A, Benli I, Demir O. Assessment of local and systemic
25-hydroxy-vitamin D, RANKL, OPG, and TNF levels in patients with rheumatoid arthritis and
periodontitis. J Oral Sci. 2017; 59(3): 397–404. doi: 10.2334/josnusd.16-0677

19. Marahleh A, Kitaura H, Ohori F, Kishikawa A, Ogawa S, Shen WR, et al. TNF-α Directly
Enhances Osteocyte RANKL Expression and Promotes Osteoclast Formation. Front
Immunol. 2019; 10(December): 1-7. doi: 10.3389/fimmu.2019.02925

20. Algate K, Haynes DR, Bartold PM, Crotti TN, Cantley MD. The effects of tumour necrosis
factor-α on bone cells involved in periodontal alveolar bone loss; osteoclasts, osteoblasts
and osteocytes. J Periodontal Res. 2016; 51(5): 549–566. doi: 10.1111/jre.12339

21. Olszewska-Czyz I, Firkova E. Vitamin D3 serum levels in periodontitis patients: a case–
control study. Med. 2022; 58(5): 585. doi: 10.3390/medicina58050585

22. Isola G, Alibrandi A, Rapisarda E, Matarese G, Williams RC, Leonardi R. Association
of vitamin D in patients with periodontitis: A cross-sectional study. J Periodontal Res.
2020;55(5): 602–612. doi: 10.1111/jre.12746

23. Khammissa RAG, Ballyram R, Jadwat Y, Fourie J, Lemmer J, Feller L. Vitamin D
Deficiency as It Relates to Oral Immunity and Chronic Periodontitis. Sabbah W, editor. Int J
Dent. 2018; 2018: 7315797. doi: 10.1155/2018/7315797

24. Lee DE, Won SY. Relationship between clinical indicators of periodontal disease and
serum level of Vitamin D. Curr Res Nutr Food Sci. 2019; 7(1): 29–40.
doi: 10.12944/CRNFSJ.7.1.04

25. Dragonas P, El-Sioufi I, Bobetsis YA, Madianos PN. Association of Vitamin D with
periodontal disease: A narrative review. Oral Heal Prev Dent. 2020; 18(2): 103–14.
doi: 10.3290/j.ohpd.a44323

26. Stein SH, Livada R, Tipton DA. Re-evaluating the role of vitamin D in the periodontium. J
Periodontal Res. 2014; 49(5): 545–553. doi: 10.1111/jre.12149



DOI: https://doi.org/10.22146/majkedgiind.81563

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