* Corresponding Author
Rapid enamel deposition on Sprague Dawley after nano calcium supplementation during pregnancy
https://doi.org/10.22146/majkedgiind.37412 Heriati Sitosari(1*), Alma Linggar Jonarta(2), Yustina Andwi Ari Sumiwi(3), Tetiana Haniastuti(4)
(1) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Histology and Cellular Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta
(4) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
Calcium is one of the most important minerals needed during hard tissue development. The preparation of this material into nano-sized particle is carried out to enhance the bioavailability and distribution of calcium in the body. Lack of calcium during odontogenesis causes defect in enamel such as hypoplasia and hypomineralization. During amelogenesis, after secretion of organic matrices, enamel mineralization will start in the presence of calcium. The objective of this study was to determine the effect of nano calcium supplementation during pregnancy on enamel development. In this study, 3-month-old female Sprague Dawley were mated and divided into three groups: nano calcium group (A), micro calcium group (B), and negative control group (C). The treatment was started on day 1 of pregnancy to day 1 after birth by intragastric administration method. The mandibles of 6 pups from each group were collected and stained with hematoxylin and eosin. Examination was conducted using microscope. Enamel deposition was measured using Optilab Image Raster® and the data collected was analyzed using t-test. Histological section of mandibular right first molar on Sprague Dawley newborn pups showed that enamel was observed on day 1 after birth but only on the group treated with nano calcium and micro calcium. Statistical analysis performed showed that the difference between the two groups was significant (p<0.05). From this study it can be concluded that the administration of nano calcium during pregnancy leads to rapid enamel deposition on Sprague Dawley pups.
Keywords
Full Text:
PDF-1nReferences
1. Vavrusova M, Skibsted LH. LWT - Food Science and Technology Calcium nutrition.
Bioavailability and forti fi cation. LWT - Food Sci Technol. 2014; 59: 1198–1204.
2. Gharibzahedi SMT, Jafari SM. The importance of minerals in human nutrition: Bioavailability, food fortification, processing effects, and nanoencapsulation. Trends Food Sci Technol. 2017; 62(2017): 119–132.
3. Kardos TB, Hunter AR, Hanlin SM, Kirk EE. Odontoblast differentiation: a response to environmental calcium? Endod Dent Traumatol. 1998; 14(3): 105–111.
4. Hirayama S, Komine C, Takahashi C, Matsui S, Matsushima K. Effects of calcium carbonate
on odontoblast differentiation and calcification ability of human dental pulp cells. J Oral Tissue Engin. 2013; 11(2): 123–134.
5. Chaudhry Q, Scotter M, Blackburn J, Ross B, Boxall A, Castle L, et al. Applications and
implications of nanotechnologies for the food sector. Food Addit Contam Part A Chem
Anal Control Expo Risk Assess. 2008; 25(3): 241–258.
6. Rizvi SSH, Moraru CI, Bouwmeester H, Kampers FWH. Nanotechnology and Food
Safety. In: Boisrobert CE, Stjepanovic A, Oh S, Lelieveld HLM, editors. Ensuring Global
Food Safety: Exploring Global Harmonization. Amsterdam: Academic Press; 2010.
7. Jeong MS, Cho HS, Park SJ, Song KS, Ahn KS, Cho M-H, et al. Physico-chemical
characterization-based safety evaluation of nanocalcium. Food Chem Toxicol. 2013;
62(2013): 308–317.
8. Hagens WI, Oomen AG, De Jong WH, Cassee FR, Sips AJ. What do we (need to) know about the kinetic properties of nanoparticles in the body? Regul Toxicol Pharmacol. 2007; 49(3): 217–229.
9. De Jong WH, Hagens WI, Krystek P, Burger MC, Sips AJ, Geertsma RE. Particle sizedependent organ distribution of gold nano particles after intravenous administration. Biomaterials. 2008; 29(12): 1912–1919.
10. Buchowski MS. Calcium in the context of dietary sources and metabolism. In: Preedy VR, editor. Calcium: Chemistry, Analysis, Function, and Effects. Cambridge: The Royal Society of Chemistry; 2016.
11. Ziegler J, Mobley CC. Pregnancy, child nutrition, and oral health. In: Touger-Decker R, Mobley C, Epstein JB, editors. Nutrition and Oral Medicine. London: Humana Press; 2014.
12. Berkovitz BKB, Holland GR, Moxham BJ. Oral anatomy, histology, and embryology. 4th ed. London: Mosby Elsevier; 2009.
13. Berkovitz BKB, Moxham BJ, Linden RWA, Sloan AJ. Master dentistry volume three:
oral biology. Third Edit. London: Churchill Livingstone Elsevier; 2011.
14. Otto GM, Franklin CL, Clifford CB. Biology and diseases of rats. In: Fox JG, Anderson LC,
Otto GM, Pritchett-Corning KR, Whary MT, editors. Laboratory Animal Medicine. 3rd ed.
Amsterdam: Academic Press; 2015.
15. Gaete M, Lobos N, Torres-Quintana M. Mouse tooth development time sequence determination for the ICR/Jcl strain. J Oral Sci. 2004; 46(3): 135–141.
16. Ziegler J, Mobley CC. Pregnancy, child nutrition, and oral health. In: Touger-Decker R,
Mobley CC, Epstein JB, editors. Nutrition and Oral Medicine. New York: Springer Science;
2014.
17. Ornitz DM, Itoh N. The fibroblast growth factor signaling pathway. WIREs Dev Biol. 2015; 4: 215–266.
18. Zheng L, Ehardt L, McAlpin B, About I, Kim D, Papagerakis S, et al. The tick tock of
odontogenesis. Exp Cell Res. 2014; 325: 83–89.
19. Nurbaeva MK, Eckstein M, Concepcion AR, Smith CE, Srikanth S, Paine ML, et al.
Dental enamel cells express functional SOCE channels. Sci Rep. 2015; 5(15803): 1–10.
20. Nanci A. Ten Cate’s Oral Histology. 9th ed. Missouri: Elsevier; 2017.
21. Tsai F-C, Kuo G-H, Chang S-W, Tsai P-J. Ca2+ signaling in cytoskeletal reorganzation,
cell migration, and cancer metastasis. BioMed Res Int. 2015; 2015: 1–13.
22. Matalova E, Lungova V, Sharpe P. Development of tooth and associated structures. In: Vishwakarma A, Sharpe P, Shi S, Ramalingam M, editors. Stem Cell Biology and Tissue Engineering in Dental Sciences. 1st ed. Cambridge: Academic Press; 2014.
23. Li YC, Pirro AE, Demay MB. Analysis of vitamin D-dependent calcium-binding protein
messenger ribonucleic acid expression in mice lacking the vitamin D receptor. Endocrinology.
1998; 139(3): 847–851.
24. Christakos S, Dhawan P, Porta A, Mady LJ, Seth T. Vitamin D and intestinal calcium
absorbtion. Mol Cell Endocrinol. 2011; 347(1–2): 25–29.
DOI: https://doi.org/10.22146/majkedgiind.37412
Article Metrics
Abstract views : 2161
| 
views : 1851
Refbacks
- There are currently no refbacks.
Copyright (c) 2018 Majalah Kedokteran Gigi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.