* Corresponding Author
Anti-inflammatory role of papaya seed extracts in inhibiting osteoclastogenesis of rats with Periodontitis
https://doi.org/10.22146/majkedgiind.26829 Ratih Pusporini(1*), Ahmad Basori(2), Agung Krismariono(3)
(1) Department of Oral Biology, Faculty of Dentistry, Universitas Brawijaya, Malang, East Java
(2) Department of Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java
(3) Department of Periodontics, Faculty of Dentistry, Universitas Airlangga, Surabaya, East Java
(*) Corresponding Author
Abstract
Some previous studies mentioned that papaya seed extract has anti-inflammatory activity because it contains polyphenols including phenolic and flavonoid acids. Polyphenols of the papaya seed extracts are known to inhibit the severity of periodontitis. It can prevent the release of excessive osteoclastogenesis which may promote bone resorption aggravating periodontitis. Hence, this study aims to determine the role of papaya seed extract in reducing the number of osteoclasts in rats with periodontitis. Twenty-eight male Wistar rats were divided into 4 groups of induced periodontitis using lipopolisakarida (LPS) Porphyromonas gingivalis (P. Gingivalis) and followed by wire ligature in “8” placed around the mandible mandibular incisor. The first group was given standard food without any papaya extract, while each rat in three other groups with periodontitis (P2, P3, P4) was given with Papaya seed extracts of 200 mg/kgBW, 300 mg/kgBW, and 400 mg/kgBW. Seven rats in one group were not induced by periodontitis and were fed with standard food as a control group. On the 30th day, the rat was sacrificed, and its transverse pieces of alveolar bone around the mandibular incisor were stained with Hematoxylin-Eosin. The data analyzed by one way ANOVA indicated a significant result. Thus, it is conclusive that extract of papaya seed ethanol inhibited osteoclastogenesis in Wistar rats induced with periodontitis.
Keywords
Full Text:
PDFReferences
1. Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, Hernandez M, Gamonal J. Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015; 23(3): 329–355. doi: 10.1590/1678-775720140259.
2. Kamer AR, Craig RG. A clinician’s guide to sys- temic effects of periodontal diseases. 2016. 93-106. Available from: http://link.springer. com/10.1007/978-3-662-49699-2.
3. Hienz SA, Paliwal S, Ivanovski S. Mechanisms of bone resorption in periodontitis. J Immunol Res. 2015; 2015: 615486. doi: 10.1155/2015/615486
4. Leyva-Lopez N, Gutierrez-Grijalva EP, Am- briz-Perez DL, Heredia JB. Flavonoids as cytokine modulators: A possible therapy for inflammation-related diseases. Int J Mol Sci. 2016;17(6). doi: 10.3390/ijms17060921.
5. Amazu LU, Azikiwe CCA, Njoku CJ, Osuala FN, Nwosu PJC, Ajugwo AO, Enye JC. Antiinflammatory activity of the methanolic extract of the seeds of Carica papaya in experimental animals. Asian Pac J Trop Med. 2010; 3(11):884–886. doi: 10.1016/S1995-7645(10)60212-X.
6. Ionel A, Lucaciu O, Moga M, Buhatel D, Ilea A, Tabaran F, Catoi C, Berce C, Toader S, Campian RS. Periodontal disease induced in Wistar rats - experimental study. HVM Bioflux. 2015; 7(2): 90–95.
7. Guimarães MR, De Aquino SG, Coimbra LS, Spolidorio LC, Kirkwood KL, Rossa C. Curcumin modulates the immune response associated with LPS-induced periodontal disease in rats. Innate Immun. 2012; 18(1): 155–163. doi: 10.1177/1753425910392935.
8. Prasetya RC, Purwanti N, Haniastuti T. Infiltrasi neutrofil pada tikus dengan periodontitis se- telah pemberian ekstrak etanolik kulit mang- gis. Majalah Kedokteran Gigi Indonesia. 2014; 21(1): 33–38.
9. Köse O, Arabaci T, Kizildag A, Erdemci B, Özkal ED, Gedikli S, Özkanlar S, Zihni M, Albayrak M, Kara A, Kermen E. Melatonin prevents radiation-induced oxidative stress and periodontal tissue breakdown in irradiated rats with experimental periodontitis. J Periodontal Res. 2017; 52(3): 438–446. doi: 10.1111/jre.12409
10. Kostić M, Kitić D, Petrović MB, Jevtović- Stoimenov T, Jović M, Petrović A, Živa- nović S. Anti-inflammatory effect of the Salvia sclarea L. ethanolic extract on lipopolysaccharide-induced periodontitis in rats. J Ethnopharmacol. 2017; 199: 52–59. doi: 10.1016/j.jep.2017.01.020.
11. Rajendrasozhan S, Yang S-R, Edirisinghe I, Yao H, Adenuga D, Rahman I. Deacetylases and NF- κ B in Redox Regulation of Cigarette Smoke-Induced Lung Inflammation: Epige- netics in Pathogenesis of COPD. Antioxid Redox Signal. 2008; 10(4): 799–812. doi: 10.1089/ars.2007.1938.
12. Dudarić L, Fužinac-Smojver A, Muhvić D, Giacometti J. The role of polyphenols on bone metabolism in osteoporosis. 2015; 77(2): 290–298. doi: 10.1016/j.foodres.2015.10.017.
13. Benso B, Franchin M, Massarioli AP, Paschoal JAR, Alencar SM, Franco GCN, Rosalen PL. Anti-inflammatory, anti-osteoclastogenic and antioxidant effects of malva sylvestris extract and fractions: In vitro and in vivo studies. PLoS One. 2016; 11(9): 1–19. doi: 10.1371/journal.pone.0162728.
14. Trzeciakiewicz A, Habauzit V, Horcajada MN. When nutrition interacts with osteoblast function: Molecular mechanisms of poly- phenols. Nutr Res Rev. 2009; 22(1): 68–81. doi: 10.1017/S095442240926402X.
15. Kumar P, Ansari SH, Ali J. Herbal remedies for the treatment of periodontal disease - a patent review. Recent Pat Drug Deliv Formul. 2009; 3(3): 221–228. doi: 10.2174/187221109789105603.
16. Maruyama T, Tomofuji T, Endo Y, Irie K, Azuma T, Ekuni D, Tamaki N, Yamamoto T, Morita Met. Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation. Arch Oral Biol. 2011; 56(1): 48–53. doi: 10.1016/j.archoralbio.2010.08.015.
17. Subhashini R, Mahadeva Rao U, Sumathi P, Gunalan G. A comparative phytochemical analysis of cocoa and green tea. 2010; 3(2): 188–192.
DOI: https://doi.org/10.22146/majkedgiind.26829
Article Metrics
Abstract views : 4640
| 
views : 3256
Refbacks
- There are currently no refbacks.
Copyright (c) 2018 Majalah Kedokteran Gigi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.