Aktivitas Antimikroba Ekstrak Etil Asetat Fungi Endofit Eutypa linearis
Baiq Maylinda Gemantari(1), Fitra Romadhonsyah(2), Arief Nurrochmad(3), Subagus Wahyuono(4), Puji Astuti(5*)
(1) Fakultas Farmasi, Universitas Gadjah Mada Departemen Farmasi, Fakultas Kesehatan, Universitas Hamzanwadi
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Pharmacology and Clinical Pharmacy Department, Faculty of Pharmacy, Universitas Gadjah Mada
(4) Pharmaceutical Biology Department, Faculty of Pharmacy, Universitas Gadjah Mada
(5) Pharmaceutical Biology Department, Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Fungi endofit banyak dilaporkan mampu memproduksi senyawa metabolit yang memiliki beragam aktivitas biologis. Diisolasi dari salah satu tanaman berkhasiat Indonesia yang dikenal dengan nama tanaman jinten (Coleus amboinicus Lour.), pada penelitian ini dilakukan observasi aktivitas antimikroba fungi endofit Eutypa linearis. Ekstrak etil asetat dari media fermentasi E.linearis dalam beragam konsentrasi diujikan kepada beberapa mikroba bakteri dan fungi yakni Staphyloccocus aureus, Pseudomonas aeruginosa, Bacillus subtilis, dan Candida albicans. Aktivitas antimikroba dievaluasi menggunakan metode mikrodilusi pada media Mueller Hinton Broth untuk kultur bakteri dan Brain Heart Infussion Broth untuk kultur fungi. Nilai optical density pada panjang gelombang 600 nm yang diperoleh dari penggunaan microplate reader digunakan untuk menghitung nilai IC50 ekstrak. Hasil penelitian menunjukkan nilai IC50 ekstrak etil asetat media fermentasi E.linearis pada S.aureus adalah sebesar 260,69 µg/mL; P.aeruginosa 359,32 µg/mL; B.subtilis 163,74 µg/mL; dan C.albicans 92,75 µg/mL. Berdasarkan metode bioautografi kontak, diketahui senyawa aktif sebagai antimikroba tersebut merupakan kelompok senyawa yang polar.
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DOI: https://doi.org/10.22146/farmaseutik.v18i3.64133
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