Formula Optimization of o/w Cream Combination Oxybenzone and Titanium Dioxide and Its In Vivo Activity Testing

Ribka Elcistia; Abdul Karim Zulkarnain

doi:10.22146/farmaseutik.v14i2.42596

Formula Optimization of o/w Cream Combination Oxybenzone and Titanium Dioxide and Its In Vivo Activity Testing

https://doi.org/10.22146/farmaseutik.v14i2.42596

Ribka Elcistia⁽¹⁾, Abdul Karim Zulkarnain^(2*)

(1) Fakultas Farmasi, Universitas Gadjah Mada Yogyakarta
(2) Fakultas Farmasi, Universitas Gadjah Mada Yogyakarta
(*) Corresponding Author

Abstract

The downside effects from sunlight exposure can be reduced by using sunscreen. Emulsifier optimization of triethanolamine (TEA) stearate and cetyl alcohol in sunscreen cream containing combination of oxybenzone and titanium dioxide is expected to produce the desired cream optimum formula with good physical stability. This study aimed to determine the optimum TEA sterate and cetyl alcohol proportion and figure out the SPF value of o/w cream containing combination of oxybenzone and titanium dioxide. The optimum formula was obtained based on cream physical characteristics testing with Simplex Lattice Design method using software Design Expert version 9.0.4. One sample t-test was used to determine the difference of optimum formula value between research and software Design Expert version 9.0.4 analysis. The comparison between optimum formula o/w cream combination oxybenzone and titanium dioxide during 4 weeks storage was analyzed using ANOVA test. The SPF value testing through in vivo was conducted against female rabbits strain New Zealand White induced by 8-methoxyprosalen. The result showed that proportion TEA stearate and cetyl alcohol which produced the optimum formula of o/w cream combination oxybenzone and titanium dioxide were 8.93% and 2.07%. The viscosity and adhesiveness were not siginifically different, while cream spreadability was significally different during 4 weeks storage. Testing activity in vivo sunscreen cream o / w combination of oxybenzone and titanium dioxide produced SPF 12.

Keywords

oxybenzone, titanium dioxide, sunscreen, in vivo

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References

Abdurahman, H. N. & Rosli, M. Y., 2006, Stability Investigation of Water-in-Crude Oil Emulsion, Journal of Applied Science, 6, 2895-2900. Agoes, G., 2012, Sediaan Farmasi Likuida-Semisolida (SFI-7), Penerbit ITB, Bandung. Ameliana, L., Oktora, L. & Maharani, Z., 2012, Optimasi Komposisi Asam Laktat dan Zink Oksida dalam Krim Tabir Surya Kombinasi Benzophenone-3 dan Octyl methoxycinnamate, Skripsi, Fakultas Farmasi Universitas Jember, Jember. Amnuaikit, T. & Boonme, P., 2015, Formulation and Characterization of Sunscreen Creams with Synergistic Efficacy on SPF by Combination of UV Filters, Journal of Applied Pharmaceutical Science, 3(8), 001-005. Anief, M., 1999, Sistem Dispersi, Formulasi Suspensi dan Emulsi, Gadjah Mada University Press, Yogyakarta. Ansel, H. C., 2005, Pengantar Bentuk Sediaan Farmasi, Edisi IV, Penerbit UI, Jakarta. Aulton, M. E., 2002, Pharmaceutics : The Science Dosage Form Design, 2nd Ed., Ed. Harcourt Publisher, New York . Barmar, M., Barikani, M. & Kaffashi, B., 2005, Steady Shear Viscosity Study of Various HEUR Models with Hydrophilic and Hydrophobic Sizes, Colloids and Surfaces A : Physicochemical and Engineering Aspects, 253, 77-82. Baughman, B. M. et al., 2009, Structural and Spectroscopic Studies of the Photophysical Properties of Benzophenone Derivatives, The Journal of Physical Chemistry A , 113(28), 8011-8019. Benson, H., 2007, Sunscreen : Efficacy, Skin Penetration, and Toxicological Aspects. Dalam: K. Walters & M. Roberts, Dermatologic, Cosmeceutic, and Cosmetic Development, 419-435, Informa Healthcare, USA. Correa, B. A. M. et al., 2012, Molecular Modeling Studies of the Structural, Electronic, and UV Absorption Properties of Benzophenone Derivatives, The Journal of Physical Chemistry A, 116, 10927-10933. Diaz-Cruz, M. S., Llorca, M. & Barcelo, D., 2008, Organic UV Filters and Their Photodegradates, Metabolites, and Disinfection By-Products in the Aquatic Environment. Trends in Analytical Chemistry, 27. Draelos, Z. D. & Thaman, L. A., 2006, Cosmetic Formulation of Skin Care Products, Informa Healthcare USA, Inc., New York. El-Boury, S. et al., 2007, Effect of The Combination of Organic and Inorganic Filters on the Sun Protection Factor (SPF) Determined by In Vitro Method, International Journal of Pharmaceutics, 1-5. Faurschou, A. & Wulf, H., 2007, The Relation Between Sun Protection Factor and Amount of Sunscreen Applied In Vivo, British Journal of Dermatology, 156, 716-719. Garg, A., Aggarwal, D., Garg, S. & Singla, A., 2002, Speading of Semisolid Formulation : An Update, Pharmaceutical Technology, 84-102. Goskonda, S. R., 2009, Dalam: R. C. Rowe, P. J. Sheskey & M. E. Quinn, Handbook of Pharmaceutical Excipients, 754-755, Pharmaceutical Press and American Pharmacists Association, USA. Sunscreen Protection, Journal of Dermatology, 20, 112-119. Hanson, K. M., Gratton, E. & Bardeen, C. J., 2006, Sunscreen Enhancment of UV-induceed Reactive Oxygen Species in the Skin, Free Radical Biology & Medicine Journal, 41, 1205. Holick, M., MacLaughlin, J., Clark, M. & Holick, S., 1980, Photosynthesis of Previtamin D3 in Human Skin and the Physiologic Consequences, Science 210, 203-205. Hussein, M. R., 2005, Ultraviolet Radiation and Skin Cancer: Molecular Mechanisms, Journal of Cutaneous Pathology , 3, 191-205. Issa, T. S. et al., 2000. Improved Kinetic Parameter Estimation in pH-Profile Data, International Journal of Pharmaceutics, 198, 39-49. Jellinek, J. S., 1970, Formulation and Function of Cosmetics, Wiley-Interscience, New York. Jelvehgari, M., Rashidi, M. R. & Mohammadi, S. H. M., 2007, Adhesive and Spreading Properties of Pharmaceutical Gel Composed of Cellulose Polymer, Jundishapur Journal of Natural Pharmaceutical Products , 2(1), 45-58. Kim, S. & Choi, K., 2014, Occurrences, Toxicities, and Ecological Risks of Benzophenone-3, A Common Component of Organic Sunscreen Products: A Mini-Review, Environment International, 70, 143–157. Kim, S. M. et al., 2010, The Relation Between the Amount of Sunscreen Applied and the Sun Protection Factor in Asian Skin, Journal of the American Academy Dermatology, 62(2), 218-222. Kurniawan, D. W. & Sulaiman, S., 2009, Teknologi Sediaan Farmasi, Edisi IV, Graha Ilmu, Yogyakarta. Lachman, L., Lieberman, H. A. & Kanig, J. L., 1994, Teori dan Praktek Farmasi Industri II, Edisi III, Universitas Indonesia Press, Jakarta. Langenbucher & Lange, 2007, Teori dan Praktek Farmasi Industri II . Edisi III, Universitas Indonesia Press, Jakarta. Martin, A., Swarbick, J. & Cammarata, A., 1993, Farmasi Fisik 2, Edisi II, Universitas Indonesia Press, Jakarta. Marchaban, Fudholi, A., Sulaiman, T.N.S., Mufrod, Martin, R., Bestari, A.N., 2015, Seri Buku Petunjuk Praktikum Teknologi Farmasi: Teknologi Formulasi Sediaan Cair Semi Padat, Laboratorium Teknologi Farmasi Fakultas Farmasi UGM, Yogyakarta. Matousek, J. L. et al., 2003, Evaluation of the Effect of pH on In Vivo Growth of Malassezia Pachydermatis, Canadian Journal of Veterinary Research, 67, 56-59. Myers, D., 2006, Surfactant Science and Technology, 3rd Ed., John Willey and Sons Inc., New Jersey. Ningrum, A. A., 2011, Optimasi Proses Pencampuran Hand Lotion dengan Kajian Kecepatan Putar Mixer, Suhu, dan Waktu Pencampuran Menggunakan Metode Desain Faktorial, Skripsi, Universitas Sanata Dharma, Yogyakarta. Osterwalder, U. & Herzog, B., 2009, Sun Protection Factor : World Wide Confusion, British Journal of Dermatology, 161 (3), 13-24. Rosita, M. R. E., Murrukmihadi, M. & Suwarmi, 2014, Pengaruh Kombinasi Oxybenzone dan Octyl Methoxycinnamate (OMC) pada Karakteristik Fisik dan SPF dalam Sediaan Krim Tabir Surya, Majalah Farmaseutik , 10(1), 182-186. Rowe, R., Sheskey, P. J. & Owen, S. C., 2005, Handbook of Pharmaceutical Excipients, 5th Ed., Pharmaceutical Press, London. Sambandan, D. R. & Ratner, D., 2011, Sunscreens : An Overview and Update, Journal of the American Academy of Dermatology, 64, 748-758. Sastrohamidjojo, H., 1991, Spektroskopi, Liberty, Yogyakarta. Sekardani, N. I., 2011, Stabilitas Fisik Sediaan Krim Pati Bengkuang (Pachyrhizus erosus (L.) Urban) dan Aktivitasnya Sebagai Tabir Surya pada Mencit, Skripsi, Fakultas Farmasi Univeristas Gadjah Mada, Yogyakarta. Sheu, M. T. et al., 2003, Correlation of In vivo and In vitro Measurements, Journal of Food and Drug Analysis, 11(2), 128-132. Sinko, P. J., 2006, Physical Pharmacy and Pharmaceutical Sciences, 5th Ed., Lippincott William & Wilkins, Philadelphia. Sliney, D. H., 2001, Photoprotection of the Eye UV Radiation and sunglasses, Journal Photochemical & Photobiology B, 64, 166-175. Smith, E. W., Maibach, H. I. & Surber, C., 2000, Use of Emulsions as Topical Drug Delivery Systems, In: F. Nielloud & G. Marti-Mestres, (Ed.), Pharmaceutical Emulsions and Suspensions , 259-270, Marcel Dekker, New York. Swarbick, J. & Boylan, J. C., 1996, Encyclopedia of Pharmaceutical Technology, 14th Ed., Marcel Dekker Inc., New York. Swarbrick, J. & Boylan, J., 1995, Percutaneous Absorption, Dalam: Encyclopedia of Pharmaceutical Technology, 413-445, Marcel Dekker Inc., New York. Tian, Y. et al., 2014, The Injury and Cumulative Effects on Human Skin by UV Exposure from Artificial Fluorescence Emission, Photochemistry and Photobiology, 90,1433-1438. Voigt, R., 1984, Buku Pelajaran Teknologi Farmasi, Edisi V, Gadjah Mada University Press, Yogyakarta.

DOI: https://doi.org/10.22146/farmaseutik.v14i2.42596