Effect of Tithonia Diversifolia (Hemsley) A.Gray and Curcuma Domestica Val Extracts Combination Against Nodule and Body Weight In Cancer Rats Model

Mae Sri Hartati Wahyuningsih; Wahyu Nur Halimah; Woro Rukmi Prtiwi

doi:10.22146/farmaseutik.v13i1.38465

Effect of Tithonia Diversifolia (Hemsley) A.Gray and Curcuma Domestica Val Extracts Combination Against Nodule and Body Weight In Cancer Rats Model

https://doi.org/10.22146/farmaseutik.v13i1.38465

Mae Sri Hartati Wahyuningsih^(1*), Wahyu Nur Halimah⁽²⁾, Woro Rukmi Prtiwi⁽³⁾

(1) Departemen Farmakologi dan Terapi, Fakultas Kedokteran UGM
(2) Mahasiswa Program Studi Pendidikan Dokter FK-UGM
(3) Departemen Farmakologi dan Terapi, Fakultas Kedokteran UGM
(*) Corresponding Author

Abstract

Kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] is a potentially anticancer plants that have been previously shown have cytotoxics effect on WiDr colon cancer cells, IC₅₀=61.55µg/mL. Turmeric (Curcuma domestica Val.) is widely used in traditional medicine. Curcuminoids compounds from turmeric have potential anticancer activity with possibly modulate immune function mediated by lymphocytes. With the immunostimulant effect of turmeric when mixed with kembang bulan as an anticancer candidate is expected to synergize better. This study aims to determine the potential of T. diversifolia and C. domestica ethanolic extracts combination in cancer rats model by measuring weight, number of nodules, and nodule volume animals. The study was a quasi-experimental study. Mixed 70% ethanol extract of kembang bulan’s leaves and turmeric’s rhizome divided into 3 doses, 40:150mg, 80:150mg, and 160:150mg. Then it given to each group of rats by orally that had experienced breast cancer . After that, see potential on body weight, number of nodules, and nodule volume. Then compared with normal group, negative control group, and positive control group. Furthermore, the percentage change in each treatment group were compared and statistically tested using ANOVA analysis. Comparison of the results of rat’s body weight between the control group (doxorubicin) and test group showed significantly no different results. Comparison of the results of rat’s breast cancer nodule volume between the control group and the test group showed significantly no different results. A combination of 70% ethanol extract of kembang bulan’s leaves and turmeric’s rhizome have no significant effect on body weight and breast cancer nodules.

Keywords

Tithonia diversifolia, Curcuma domestica, DMBA, body weight, cancer nodules

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References

Baruah, N.C., Sharma, R.P., Madhusudana, K.P., Thygarajan, Gopalakrisna, Werner, H., Murary, Ramaswarny, 1979, Sesquiterpen Lactones of Tithonia diversifolia A. Gray. Chemical Abstract, 91 (20751): p.678, American Chemical Society, Ohio. Churchill, M. 2000. Inhibition of intestinal tumors by curcumin is associated with changes in the intestinal immune cell profile. J Surg Res, 89:169-75. Fürstenberger G, Krieg P, Müller-Decker K, Habenicht AJ, 2007. What are cyclooxygenases and lipoxygenases doing in the driver's seat of carcinogenesis? Int J Cancer, 119: 2247-54. Goffin, E., Ziemons, E., de Mol, P., de MadureiraMdo, C., Martins, A.P., da Cunha, A.P., Phillippe, G., Tits, M., Angenot, L., and Frederich, M., 2002. In vitro antiplasmodium activity of Tithonia diversifolia and Identification of its main active constituent :Taginin C., Planta Med. 68 (6): 543-5. Gu, J. Q., Gills, JJ., Park, EJ., Mata-Greenwood, E., Hawthorne, ME., Axelrod, F., Chavez, PI., Fong, HH., Mehta, RG., Pezzuto, JM. and Kinghorn, AD., 2002, Sesquiterpenoids from Tithonia diversifolia with potencial care chemopreventive activity, J Nat Prod, 65(4): 532-536. Howland, R.D., Mycek, M.J., editors Harvey, R.A., Champe, P. C., 2006. Pharmacology 3rd ed ( Lippincot’s illustrated reviews). Lippincott Williams & Wilkins, India. Ibrahim, A., El-Meligy, A., Lungu, G., Fetaih, H., Dessouki, A., Stoica, G., Barhoumi, R., 2011, Curcumin induces apoptosis in a murine mammary gland adenocarcinoma cell line through the mitochondrial pathway. Eur J Pharmacol. 668(1-2):127-32. Meiyanto, E., 1999, Kurkumin Sebagai Obat Kanker :Menelusuri Mekanisme Aksinya (Review), M. F. I., 10 (4), 224-236. Nugroho, A.E., Hermawan,A., Putri,D.D.P., Meiyanto, E., Hakim,L. 2012, Synergistic effects of ethyl acetate fraction of Ficus septic Burm.f. and doxorubicin chemotherapy on T47D human breast cancer cell line..[Pubmed : 23073201]. Nurhalimah W, 2013, Pengaruh Pemberian Ekstrak Etanol Tithonia diversifolia (Hemsley) A.Gray dan Curcuma domestica Val Terhadap Nodul Kanker Payudara dan Berat Badan Tikus Betina yang Diinduksi dengan DMBA, [Skripsi], Fakultas Kedokteran, UGM, Yogyakarta. Obafemi, C. A., Sulaimon, T. o., Akinpelu, D. A., Olugbade., 2006. Antimicrobial activity of extracts and a germacranolide-type sesquiterpene lactone from Tithonia diversifolia leaf extract. African Journal of Biotechnology 5(12):1254-8. Patil LR and Balaraman R, 2009. Effect of melatonin on Doxorubicin Induced testicular damage in rat. Intl.J.PharmTech.Res.(IJPRIF). 1(3): 879-884. Verma SP, Goldin BR, Lin PS, 1998. The inhibition of the estrogenic effects of pesticides and environmental chemicals by curcumin and isoflavonoids. Environ Health Perspect,106: 807-12. Wahyuningsih MSH, Pratiwi WR, 2012, Aktivitas Antikanker ekstrak etanol terstandar Tithonia diversifolia (Hemsley) A. Gray. dan Curcuma domestica Val. pada Tikus Model Kanker Payudara Serta Toksisitasnya, Laporan Penelitian, Damas tahun 2012, FK-UGM. Wahyuningsih MSH, Wijayanti MA, Budiyanto A, Hanafi M. 2015, Isolation and Identification of Potential Cytotoxic Compound from Kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] Leaves, Int J Pharm Pharm Sci. 7 (6): 298-301.

DOI: https://doi.org/10.22146/farmaseutik.v13i1.38465