* Corresponding Author
Spongia officinalis -Associated Pseudomonas fluorescens as a Reservoir of Bioactive Compounds: A Novel Source of Natural Anticancer Compounds
https://doi.org/10.22146/jtbb.90693 Usharani Subbiah(1), Yuvaraj Dinakarkumar(2), Madhusudhanan Jeyaraman(3*)
(1) Department of Chemistry, S.I.V.E.T. College of Arts and Science, Velachery Main Road, Gowrivakkam, Tambaram, Chennai 600 073, India
(2) Department of Biotechnology, Vel Tech High Tech Dr.Rangarajan Dr.Sakunthala Engineering College, Vel Tech Road Vel Nagar Avadi, Tamil Nadu 600062, India
(3) Department of Biotechnology, Anand Institute of Higher Technology, Kalasalingam Nagar IT Corridor, Kazhipattur, Tamil Nadu 603103, India
(*) Corresponding Author
Abstract
Marine sponges are an important source of chemical variety and a repository of biodiversity. In this study, the microbial communities found in the marine sponge Spongia officinalis that was taken from the Kanyakumari coast in India were explored. We identified, characterized, and evaluated the bioactive potential of the sponge-associated bacteria. A total of 12 bacterial isolates were obtained, primarily consisting of gram-positive rods and some gram-negative rods and cocci. These bacterial isolates showed a variety of bioactive substances when their secondary metabolites were extracted, including phenols, flavonoids, alkaloids, saponins, terpenoids, and steroids. These chemical compounds are well-known for their numerous biological activity and health benefits. The antioxidant and anticancer effects of these metabolites were also investigated. Isolate 6 showed significant antioxidant activity, suggesting it may be able to treat cancer. With an IC50 value of 55.32 g/ml, this isolate also displayed impressive anticancer activity against HeLa cells. The screened isolate was identified as Pseudomonas fluorescens strain ATCC 13525 using 16S rRNA sequencing. This discovery emphasizes the importance of bacteria associated with marine sponges as a source of bioactive compounds with medicinal potential. The conclusion of this research highlights the diverse microbial communities found in marine sponges and their potential for use in biotechnology and medication development. Pseudomonas fluorescens was found to be a prolific generator of bioactive byproducts, including strong antioxidants and anticancer agents, which emphasizes the significance of additional marine resource exploitation for pharmacologically relevant chemicals.
Keywords
Full Text:
PDFReferences
Arasu, M.V. et al., 2014. In vitro antimicrobial potential of organic solvent extracts of novel actinomycetes isolated from forest soil. African Journal of Biotechnology, 13(18), pp.1891-1987. doi: 10.5897/AJB2013.13555.
Arunachalam, K. & Appadorai, R.A.J., 2013. Antioxidant potential and biochemical evaluation of metabolites from the marine bacteria Virgibacillus sp. associated with the sponge Callyspongia diffusa. Free Radicals and Antioxidants, 3(1), pp.47–51. doi: 10.1016/j.fra.2013.04.004.
Bhakuni, D.S. & Rawat, D.S., 2006. Bioactive marine natural products, Springer Science & Business Media.
Brinkmann, C.M., Marker, A. & Kurtböke, D.I., 2017. An overview on marine sponge-symbiotic bacteria as unexhausted sources for natural product discovery. Diversity, 9(4), 40. doi: 10.3390/d9040040.
Ćetković, H., Halasz, M. & Herak Bosnar, M., 2018. Sponges: A reservoir of genes implicated in human cancer. Marine drugs, 16(1), 20. doi: 10.3390/md16010020.
Chelossi, E. et al., 2004. Characterisation and antimicrobial activity of epibiotic bacteria from Petrosia ficiformis (Porifera, Demospongiae). Journal of experimental marine biology and ecology, 309(1), pp.21–33. doi: 10.1016/j.jembe.2004.03.006.
Cheng, C., 2017. Metabolomics and dereplication-based isolation of novel bioactive natural products from marine sponge-associated actinomycetes. Universität Würzburg.
Elmanama, I.A. et al., 2020. In vitro Anticancer Activity Effect of Extracellular Metabolites of Some Bacterial Species on HeLa Cell Line. IUG Journal of Natural Studies, 28(2). doi: 10.33976//IUGNS.28.2/2020/2.
Fenical, W., 1993. Chemical studies of marine bacteria: developing a new resource. Chemical Reviews, 93(5), pp.1673–1683. doi: 10.1021/cr00021a001.
Harborne, J.B., 1973. Textbook of phytochemical methods, 1st Edn, Champraan and Hall Ltd. London., pp.110–113.
Jin, L. et al., 2014. Pezizomycotina dominates the fungal communities of South China Sea Sponges Theonella swinhoei and Xestospongia testudinaria. FEMS Microbiology Ecology, 90(3), pp.935–945. doi: 10.1111/1574-6941.12446.
Karthikeyan, A., Joseph, A. & Nair, B.G., 2022. Promising bioactive compounds from the marine environment and their potential effects on various diseases. Journal of Genetic Engineering and Biotechnology, 20(1), pp.1–38. doi: 10.1186/s43141-021-00290-4.
Kennedy, J. et al., 2009. Isolation and analysis of bacteria with antimicrobial activities from the marine sponge Haliclona simulans collected from Irish waters. Marine biotechnology, 11, pp.384–396. doi: 10.1007/s10126-008-9154-1.
Koopmans, M., Martens, D. & Wijffels, R.H., 2009. Towards commercial production of sponge medicines. Marine drugs, 7(4), pp.787–802. doi: 10.3390/md7040787.
Krishnan, K.A.A. & Keerthi, T.R., 2016. Analyses of Methanol Extracts of two Marine Sponges, Spongia officinalis var. ceylonensis and Sigmadocia carnosa from Southwest Coast of India for their Bioactivities. Int. J. Curr. Microbiol. App. Sci, 5(2), pp.722–734. doi: 10.20546/ijcmas.2016.502.081.
Kumar, P. et al., 2013. Extending the limits of Bacillus for novel biotechnological applications. Biotechnology advances, 31(8), pp.1543–1561. doi: 10.1016/j.biotechadv.2013.08.007.
Li, Y.-X., Himaya, S.W.A. & Kim, S.-K., 2013. Triterpenoids of marine origin as anti-cancer agents. Molecules, 18(7), pp.7886–7909. doi: 10.3390/molecules18077886.
Liu, D. et al., 2015. Microbacterins A and B, new peptaibols from the deep sea actinomycete Microbacterium sediminis sp. nov. YLB-01 (T). Organic letters, 17(5), pp.1220–1223. doi: 10.1021/acs.orglett.5b00172.
Maloney, K.N. et al., 2009. Lodopyridone, a structurally unprecedented alkaloid from a marine actinomycete. Organic letters, 11(23), pp.5422–5424. doi: doi.org/10.1021/ol901997k.
Manzo, E. et al., 2011. Bioactive terpenes from Spongia officinalis. Journal of natural products, 74(5), pp.1241–1247. doi: 10.1021/np200226u.
Migliuolo, A. et al., 1990. New tetrahydroxylated sterols from the marine sponge Spongia officinalis. Journal of natural products, 53(6), pp.1414–1424. doi: 10.1021/np50072a003.
Mohan, C.D. et al., 2022. Bacteria as a treasure house of secondary metabolites with anticancer potential. Seminars in cancer biology, 86(Pt 2), pp. 998–1013. doi: 10.1016/j.semcancer.2021.05.006.
Mostafa, Y. et al., 2019. Enhanced production of glutaminase-free L-asparaginase by marine Bacillus velezensis and cytotoxic activity against breast cancer cell lines. Electronic Journal of Biotechnology, 42, pp.6–15. doi: 10.1016/j.ejbt.2019.10.001
Photolo, M.M. et al., 2020. Antimicrobial and antioxidant properties of a bacterial endophyte, Methylobacterium radiotolerans MAMP 4754, isolated from Combretum erythrophyllum seeds. International journal of microbiology, 2020, 9483670. doi: 10.1155/2020/9483670
Prastiyanto, M.E. et al., 2022. Bioprospecting of bacterial symbionts of sponge Spongia officinalis from Savu Sea, Indonesia for antibacterial potential against multidrug-resistant bacteria. Biodiversitas Journal of Biological Diversity, 23(2), pp.1118-1124. doi: 10.13057/biodiv/d230256.
Rao, K.V.R. & Rao, T.R., 2013. Molecular characterization and its antioxidant activity of a newly isolated Streptomyces coelicoflavus BC 01 from mangrove soil. Journal of Young Pharmacists, 5(4), pp.121–126. doi: 10.1016/j.jyp.2013.10.002.
Riguera, R., 1997. Isolating bioactive compounds from marine organisms. Journal of Marine Biotechnology, 5, pp.187–193.
Shivale, N. et al., 2018. Screening of Antioxidant Activity of marine bacteria isolated from marine soil obtained from North-West coastal region of India. Int. J. Biol. Pharm. Allied Sci, 7. doi: 10.31032/IJBPAS/2018/7.3.4382.
Srinivasan, R. et al., 2021. Marine bacterial secondary metabolites: A treasure house for structurally unique and effective antimicrobial compounds. Marine Drugs, 19(10), 530. doi: 10.3390/md19100530.
Stabili, L. et al., 2008. Evaluation of microbiological accumulation capability of the commercial sponge Spongia officinalis var. adriatica (Schmidt)(Porifera, Demospongiae). Water research, 42(10–11), pp.2499–2506. doi: 10.1016/j.watres.2008.02.008.
Tangman, S., Govinden-Soulange, J. & Marie, D., 2015. Bioactive profile of Plakortis nigra, a sea sponge from Mauritius Islands. J. Coast. Life Med, 3, pp.44–51. doi: 10.12980/JCLM.3.201514B343.
Taylor, M.W. et al., 2007. Sponge-associated microorganisms: evolution, ecology, and biotechnological potential. Microbiology and molecular biology reviews, 71(2), pp.295–347. doi: 10.1128/mmbr.00040-06.
Tsilo, P.H. et al., 2020. Chemical composition and some biological activities of the methanolic Encephalartos ferox fruit extract. Pharmacognosy Journal, 12(5), pp.1190-1197.
Wang, E. et al., 2020. From seabed to bedside: A review on promising marine anticancer compounds. Biomolecules, 10(2), 248. doi: 10.3390/biom10020248
Webster, N.S. & Hill, R.T., 2001. The culturable microbial community of the Great Barrier Reef sponge Rhopaloeides odorabile is dominated by an $α$-Proteobacterium. Marine Biology, 138, pp.843–851. doi: 10.1007/s002270000503.
Wright, M.H., Adelskov, J. & Greene, A.C., 2017. Bacterial DNA extraction using individual enzymes and phenol/chloroform separation. Journal of microbiology & biology education, 18(2), pp.12–18. doi: 10.1128/jmbe.v18i2.1348
Zhang, H., Zhao, Z. & Wang, H., 2017. Cytotoxic natural products from marine sponge-derived microorganisms. Marine drugs, 15(3), 68. doi: 10.3390/md15030068.
DOI: https://doi.org/10.22146/jtbb.90693
Article Metrics
Abstract views : 1659
| 
views : 984
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Journal of Tropical Biodiversity and Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editoral address:
Faculty of Biology, UGM
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ISSN: 2540-9581 (online)