The Oriental Tiny Frog of the Genus Microhyla Tschudi, 1839 (Amphibia: Anura: Microhylidae) Revealed across Geographical Barriers of the Wallace Line

https://doi.org/10.22146/jtbb.64342

Rury Eprilurahman(1*), Vestidhia Yunisya Atmaja(2), Misbahul Munir(3), Amir Hamidy(4), Tuty Arisuryanti(5), Rosichon Ubaidillah(6)

(1) Faculty of Biology, Universitas Gadjah Mada, Indonesia
(2) Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Indonesia
(3) Graduate School of Global Environmental Studies, Kyoto University, Japan
(4) Museum Zoologicum Bogoriense, Research Center for Biology, Indonesian Institute of Science, Indonesia
(5) Faculty of Biology, Universitas Gadjah Mada, Indonesia
(6) Museum Zoologicum Bogoriense, Research Center for Biology, Indonesian Institute of Science, Indonesia
(*) Corresponding Author

Abstract


The frog genus Microhyla was considered as the South, East, and Southeast Asian frog species. Microhyla orientalis was described in 2013, distributed in Java and Bali, Indonesia. Thenceforth, it was known as the easternmost distribution of this genus within the oriental region, but recently this species was recorded from the Timor Island and Sulawesi on the Wallace regions. We applied molecular analysis to evaluate the taxonomic status  and the origin of the Wallacean population. Phylogenetic analysis using the partial 16S mitochondrial gene demonstrated that the Java, Timor and Sulawesi populations were not significantly different from the Bali population. This Wallacean population of M. orientalis was originated from Java and possibly it is accidentally distributed by humans through the expansion of agricultural activity.


Keywords


introduced species; microhyla; molecular markers; species confirmation; identification

Full Text:

PDF


References

Alhadi, F. et al., 2019. Rediscovery of Micryletta inornata (Boulenger, 1890) from Sumatra: redescription, molecular identity, and taxonomic implications. Zootaxa, 4613(1), pp.111-126.

Bandelt, H.J. et al., 1999. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16, pp.37-48

Excoffier, L. & Lischer H.E.L., 2010. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, pp.564–567.

Excoffier, L. et al., 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 131(2), pp.479–491.

Frost, D. R., 2021. Amphibian Species of the World: an Online Reference. Version 6.0 (14 Februari 2021). Electronic Database accessible at http://research.amnh.org/herpetology/amphibia/index.html. American Museum of Natural History, New York, USA.

Garg, S. et al., 2019. Systematic revision of Microhyla (Microhylidae) frogs of South Asia: a molecular, morphological, and acoustic assessment. Vertebrate Zoology, 69, pp.1–71.

Gorin, V.A. et al., 2020. A little frog leaps a long way: compounded colonizations of the Indian Subcontinent discovered in the tiny Oriental frog genus Microhyla (Amphibia: Microhylidae). PeerJ 8:e941.1, pp.1-47.

Hadi, S. et al., 2020. Genetic connectivity of the scalloped hammerhead shark Sphyrna lewini across Indonesia and the Western Indian Ocean. PLoS ONE, 15(10), pp.1–14.

Hillis, D.M. & Bull, J.J., 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, pp.182–192.

Huelsenbeck, J. P. & Hillis D. M., 1993. Success of phylogenetic methods in the four-taxon case, Systematic Biology, 42, pp.247-265

Inger, R.F. & Frogner, K.J., 1979. New species of narrow-mouth frogs (genus Microhyla) from Borneo. Sarawak Museum Journal, 27, pp.311–322.

Inger, R.F. & Stuebing, R.B., 2005. Frogs of Borneo. Natural History Publications (Borneo). Kota Kinabalu. p.201

Iskandar, D.T., 1998. Amfibi Jawa dan Bali: Seri Panduan Lapangan, Cetakan pertama. Bogor: Pusat Penelitian dan Pengembangan Biologi–LIPI. p.117.

Jobb, G. et al., 2004. Treefinder: a powerful graphical analysis environment for molecular phylogenetics. BMC Evolution Biology, 4, p.18.

Johnson, J.R. et al., 2011. The origin of tiger salamander (Ambystoma tigrinum) populations in California, Oregon, and Nevada: introductions or relicts?. Conservation Genetic, 12(3), pp. 355–370.

Kementerian Desa, Pengembangan daerah Tertinggal dan Transmigrasi RI, 2015a. Transmigrasi Masa Doeloe, kini dan Harapan Kedepan, Direktorat Bina Potensi Kawasan Transmigrasi. Jakarta. pp.1–19.

Kementerian Desa, Pengembangan daerah Tertinggal dan Transmigrasi RI, 2015b. Buku Penyelenggaraan Transmigrasi di Indonesia Tahun 1998–2014. Pusat Data dan Informasi, Badan Penelitian dan Pengembangan, Pendidikan dan Pelatihan dan Informasi. Jakarta. pp.1–70.

Kumar, S. et al., 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, pp.1547–1549.

Kurabayashi, A., 2011. From Antarctica or Asia? New colonization scenario for Australian-New Guinean narrow mouth toads suggested from the findings on a mysterious genus Gastrophrynoides. BMC Evolution Biology, 11(175), pp.1–12.

Kuraishi, N. et al., 2009. Estimation the origin of Polypedates leucomystax (Amphibia: Anura: Rhacophoridae) introduced to the Ryukyu Archipelago, Japan. Pacific Science, 63(3), pp.317–325.

Matsui, M. et al., 2005. Taxonomic relationships within the Pan–Oriental narrow–mouth toad, Microhyla ornata as revealed by mtDNA analysis (Amphibia, Anura, Microhylidae). Zoological Science, 22, pp.489–495.

Matsui, M. et al., 2011. Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylogenetics and Evolution, 61, pp.167–176.

Matsui, M., 2011. Taxonomic revision of one of the Old World’s smallest frogs, with description of a new Bornean Microhyla (Amphibia, Microhylidae). Zootaxa, 2814, pp.33–49.

Matsui, M. et al., 2013. Description of a new species of Microhyla from Bali, Indonesia (Amphibia, Anura). Zootaxa, 3670, pp.579–590.

McKay, J.L., 2006. A Field Guide to the Amphibians and Reptiles of Bali. Florida: Krieger Publishing Company. pp.33–35.

Moritz, C. et al., 1993. Genetic diversity and the history of pacific island house geckos (Hemidactylus and Lepidodactylus). Biology Journal of Linnean Society London, 48, pp.113–133.

Ota, H. et al., 2004. Feral populations of amphibians and reptiles in the Ryukyu Archipelago, Japan. Global Environmental Research, 8, pp.133–143.

Poyarkov, N.A., Jr., et al., 2020. A new cryptic species of the genus Microhyla (Amphibia: Microhylidae) from Langbian Plateau, Vietnam. Taprobanica. The Journal of Asian Biodiversity, 9, pp. 136–163.

Reilly, S.B. et al., 2017. Toxic toad invasion of Wallacea: A biodiversity hotspot characterized by extraordinary endemism. Global Change Biology, 23, pp. 5029–5031.

Reilly, S.B. et al., 2020. New Island Records for Anurans and Squamates from the Lesser Sunda Archipelago. Herpetological Review, 51(4), pp.785–789.

Ronquist, et al., 2012. MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology, 61(3), pp.539–542.

Rozas, J. et al., 2017. DnaSP v6: DNA Sequence polymorphism analysis of large datasets. Molecular Biology and Evolution. 34, pp.3299–3302.

Suzuki, D. & Hikida, T., 2011. Origin of Japanese populations of Reeves’ pond turtle, Mauremys reevesii (Reptilia: Geoemydidae), as inferred by a molecular approach. Chelonian Conservation and Biology, 10, pp.237–249.

Suzuki, D. & Hikida, T., 2014. Taxonomic Status of the Soft-Shell Turtle Populations in Japan: A Molecular Approach. Current Herpetology, 33(2), pp.171–179.

Tanabe, A.S., 2011. Kakusan4 and Aminosan: two programs for comparing nonpartitioned, proportional, and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources, 11, pp.914–921.

van Kampen, P.N., 1923. The Amphibia of the Indo–Australian Archipelago. Leiden: E. Brill Ltd. p.304.

Vences, M. et al., 2017. Tracing a toad invasion: lack of mitochondrial DNA variation, haplotype origins, and potential distribution of introduced Duttaphrynus melanostictus in Madagascar. Amphibia-Reptilia, 38, pp.197–207.

Weir, B.S & Cockerham, C.C., 1984. Estimating F-Statistics for the Analysis of Population Structure. Evolution, 38(6), pp.1358.

Weir, B.S., 1996. Genetic Data Analysis II: Methods for Discrete Population Genetic Data. Sunderland, Mass: Sinauer Associates.

Wiantoro, S. et al., 2019. Effects of the total solar eclipse of March 9, 2016 on the animal behavior. Journal of Tropical Biology and Conservation, 16, pp.137–149.

Wogan, G.O.U. et al., 2016. Deep genetic structure and ecological divergence in a widespread human commensal toad. Biology Letters, 12, 20150807, pp.1–5.

Wright, S., 1951. The genetical structure of populations. Annals of Eugenics, 15, pp.323–354.

Yudha, D. S., et. al., 2019. Keanekaragaman katak dan kodok (Amphibia: Anura) di Suaka Margasatwa Paliyan, Gunungkidul, Yogyakarta. Jurnal Biologi Udayana, 23(2), pp. 59-67.



DOI: https://doi.org/10.22146/jtbb.64342

Article Metrics

Abstract views : 3114 | views : 1931

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Editoral address:

Faculty of Biology, UGM

Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia

ISSN: 2540-9581 (online)