Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract

Putri Lestari Ningtias

doi:10.22146/jsv.92377

Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract

https://doi.org/10.22146/jsv.92377

Putri Lestari Ningtias^(1*)

(1) Diponegoro University
(*) Corresponding Author

Abstract

Agents of immunomodulation are compounds that have the ability to balance the immune system throughout the body. The neem plant is one of the herbal plants that is often used as traditional medicine. Neem leaves (Azadirachta indica A. Juss) contain antioxidants that have the potential to be antiinflammatory, antiviral, and as an immunomodulatory agent. The constraint in giving medicine orally is the low bioavailability of drugs and the distribution of active compounds of herbal plants. This problem can be solved by packaging herbal plant extracts in nanochitosan. The purpose of this study was to analyze the effect of induced of a nanochitosan ethanol extract of neem leaves on the histological structure of the spleen tissue of female Wistar white rats. This study used a randomized design (CRD) which was divided into 3 treatment groups with 4 repetitions. Treatments include control (P0), an ethanol extract of neem leaves 2 ml/head/day (P1), and nanochitosan preparation of neem leaves 2 ml/head/day (P2). The parameters measured were the diameter of white pulp, the diameter of germinal centre, the area of white pulp, and the area of germinal centre in the spleen. Data on the diameter and the area of the white pulp and germinal centre were analyzed statistically parametrically with ANOVA with a 95% level of confidence, if the data were not normally distributed, the Kruskall Wallis nonparametric test was carried out. Data that were significantly different were carried out by Duncan’s further test. The results showed that the treatment of nanochitosan preparations of neem leaf ethanol extract had a significant difference in the diameter of the white pulp and germinal centre as well as the area of the white pulp and germinal centre (P<0,05). The conclusion of this study was that the nanochitosan preparation ethanol extract of neem leaves at a dose of 14 mg/kg BB can increase the diameter and the area of the white pulp and germinal centre.

Keywords

nanochitosan; white pulp, germinal centre

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References

Abror, Y. K., E. D. Woelansari, dan S. Suhariyadi. (2018). Imunomodulator Ekstrak Etanol Daun Mimba (Azadirachta indica) terhadap Jumlah Sel Makrofag Peritoneal pada Mencit yang Diinduksi Vaksin BCG. Jurnal Teknologi Laboratorium. 7(1): 8-14.

Ali, S. A., G. Singh, and A. K. Datusalia. (2021). Potential Therapeutic Applications of Phytoconstituents as Immunomodulators: Pre‐Clinical and Clinical Evidences. Phytotherapy Research. 35(7): 3702-3731.

Braga, L. R., L. M. Perez, M. V. Soazo, and F. Machado. (2019). Evaluation of The Antimicrobial, Antioxidant, and Phsycochemical Properties of Poly (Vinyl Chloride) Films Containing Quercetin and Silver Nanoparticles. LWT-Food Sciences and Technology Elsevier. 101: 491-498.

Carmelita, Austin, B. (2016). Pengaruh Pemberian Ekstrak Etanol Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) Secara Oral pada Mencit Balb-C terhadap Pencegahan Penurunan Diameter Germinal Center pada Kelenjar Getah Bening Serta Kadar IgM Serum. J Biosains Pascasarjana. 18(1): 1-12.

Dewi, Ni, Ketut, L., M. Jamhari, dan I. Isnainar. (2017). Kajian Pemanfaatan Tanaman Sebagai Obat Tradisional di Desa Tolai Kecamatan Torue Kabupatenparigimoutong. E-Jip Biol. 5(2): 57-65.

Dewi, Parlinaningrum, S. Widyanarti, dan M. Rifai. (2013). Pengaruh Pemberian Ekstrak Etanol Annona muricata Linn terhadap Peningkatan Jumlah Sel CD4+ dan CD8+ pada Timus Mencit (Mus musculus). Biotropika. 2(5): 269-272.

Ernis, G., D. Notriawan, D. Fitriani, E. Yunita, dan I. Cantika. (2021). Uji In Vitro Aktivitas Imunomodulator Minyak Atsiri Serai Dapur (Cymbopogon citratus) terhadap Proliferasi Sel Limfosit Mencit. Bioedusains: Jurnal Pendidikan Biologi dan Sains. 4(2): 129-135.

Fathir, A., dan M. Rifa’i. (2014). Aktivitas Ekstrak Daun Kelor terhadap Sel-T Helper dan Sel-T Sitotoksik pada Mencit yang Diinfeksi Salmonella thypi. Jurnal Veteriner. 15(1): 114-122.

He, X. and H. M. Hwang. (2016). Nanotechnology in Food Science: Functionality, Applicability and Safety Assessment. J Food Drug Anal. 24(4): 671–681.

Kotala, Sarmawaty, dan T. S. Kurnia. (2022). Eksplorasi Tumbuhan Obat Berpotensi Imunomodulator di Kecamatan Leihitu Kabupaten Maluku Tengah. Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam. 19(2): 186-200.

Ku, C. M., and J. Y. Lin. 2013. Anti-Inflammatory Effects of 27 Selected Terpenoid Compounds Tested Through Modulating Th1/Th2 Cytokine Secretion Profiles Using Murine Primary Splenocytes. Food Chemistry. 141(2): 1104-1113.

Kusuma, A. B., T. R. Saraswati, dan A. J. Sitasiwi. (2019). Efek Pemberian Daun Mimba (Azadirachta indica) terhadap Diameter Hepatosit Tikus (Rattus norvegicus). Bioma: Berkala Ilmiah Biologi. 21(2): 106-113.

Makiyah, Sri, N. N., dan U. H. Wardhani. (2017). Potensi Ekstrak Etanol Buah Citrullus lanatus sebagai Agen Imunosupresi Melalui Pengamatan Histologi Limpa Mencit BALB/c. Majalah Kedokteran Bandung. 49(4): 245-251.

Makiyah, Sri, N. N., R. Iszamriach, dan A. Nofariyandi. (2014). Paparan Ultraviolet C Meningkatkan Diameter Pulpa Alba Limpa dan Indeks Mitotik Epidermis Kulit Mencit. Jurnal Kedokteran Brawijaya. 28(1): 17-21.

Matheos, C. (2014). Gambaran Histologik Jaringan Limpa Tikus Putih (Rattus norvegicus) yang Diinfeksi Eschericia coli dan Diberi Madu. Ebiomedik. 1(2): 961-965.

Meliana, Nelly. (2016). Pengaruh Pemberian Ekstrak Etanol Umbi Bawang Dayak (Eleuthrine palmifolia L.) Secara Oral pada Mencit BALB/C terhadap Pencegahan Penurunan Jumlah NK Sel Dan CD 8+. J Biosains Pascasarjana.18(1): 13-23.

Merdana, I. M., I. A. P. Hapsari, dan F. Muslih. (2020). Efektivitas Ekstrak Ethanol Daun Mimba terhadap Rhipichepalus sanguineus secara in Vitro. Buletin Veteriner Udayana. 12(1): 86-91.

Mescher, A. L. (2012). Histologi Dasar Junquiera Teks & Atlas. Jakarta. Penerbit Buku Kedokteran EGC. Hlm. 221-239.

Mozafari, M. R. M., M. Danaei, S. A. Dehghankhold, F. H. Davarani, R. Javanmard, A. Dokhani, and S. Khorasani. (2018). Impact of Particle Size and Polydispersity Index on The Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceuticals. 5:1-23.

Muslihin, A. M., R. A. Astuti, dan I. Irwandi. (2022). Analisis Aktivitas Antioksidan Fraksi Ekstrak Daun Mimba (Azadirachta indica A. Juss) dengan Metode DPPH. Jurnal Etnofarmasi. 1: 1-7.

Nata, I. D. G. A. T., N. M. Linawati, I. G. A. D. Ratnayanti, dan I. Wayan. (2021). Efek Pemberian Teh Kombinasi Bunga Euphorbia Milii dan Propolis Terhadap Diameter Pulpa Putih Limpa Tikus Wistar Jantan. Jurnal Medika Udayana. 10:5.

Perdana, P. R. (2022). Aktivitas Imunomodulator Ekstrak Herba Meniran (Phyllanthus Niruri L.). Jurnal Farmagazine. 9(1): 50-54.

Puspitaningrum, Ika, L. Kusmita, dan Y. D. Franyoto. (2017). Aktivitas Imunomodulator Fraksi Etil Asetat Daun Som Jawa (Talinum triangulare (Jacq.) Willd) terhadap Respon Imun Non Spesifik. Jurnal Ilmu Farmasi dan Farmasi Klinik. 14(1): 24-29.

Putri, Fauziah, M., A. J. Sitasiwi, S. Isdadiyanto, dan S. M. Mardiati. (2023). Profil Leukosit Tikus Jantan (Rattus novergicus L.) Galur Sprague Dawley Setelah Paparan Nanopartikel Kitosan Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss.). Jurnal Sain Veteriner. 41(1): 31-36.

Renda, G., İ. Gökkaya, and D. Şöhretoğlu. 2021. Immunomodulatory Properties of Triterpenes. Phytochemistry Reviews. 22(18): 1-27.

Rosnizar, R., K. Eriani, I. M. Ramli, dan F. Muliani. (2018). Uji Efek Imunostimulan Buah Kurma (Phoenix dactylifera) pada Mencit Jantan (Mus musculus) Galur BALB/C. In Prosiding Seminar Nasional Biotik. 3(1): 292-297.

Rosyanti, Lilin, dan I. Hadi, I. 2020. Respon Imunitas dan Badai Sitokin Severe Acute Respiratory Syndrome Corona Virus 2 Literatur Review. Jurnal Kesehatan Madani Medika. 11(02): 176–201.

Rousdy, D.W., K. R. Rahmawati, E. Kuniadi. (2017). Mikroanatomi Limpa Tikus (Rattus norvegicus L.) Setelah Pemberian Asam Humat dari Tanah Gambut Kalimantan. J Penelitian dan Pengembangan Borneo Akcaya. 4(1): 57-62.

Sarkar, S., R. P. Singh, and G. Bhattacharya. (2021). Exploring The Role of Azadirachta indica (Neem) and Its Active Compound in The Regulation of Biological Pathways: An Update on Molecular Approach. 3 Biotech Springer. 11(4): 178.

Setiawan, H., S.W. Wulandari, dan A. N. Fitriyani. (2021). Potensi Imunomodulator Herbal Ekstrak Etanol Daun Pepaya Varietas Calina terhadap Struktur Jaringan Limpa Tikus Putih Galur Wistar. Jurnal Veteriner. 22(4): 531-539.

Sholikhah, Arsela, Rindang, dan H. M. Rahayuningsih. (2015). Pengaruh Ekstrak Lompong (Colocasia esculenta L. Schoot) 30 Menit Pengukusan terhadap Aktivitas Fagositosis dan Kadar No (Nitrit Oksida) Mencit Balb/C Sebelum dan Sesudah Terinfeksi Listeria monocytogenes. Journal of Nutrition College. 4(2): 526–569.

Sitasiwi, Agung J., S. Isdadiyanto, S. M. Mardiati. (2021). Formulasi dan Evaluasi Efek Antifertilitas Sediaan Nanopartikel Esktrak Etanol Daun Mimba (SNEEDM) terhadap Penurunan Potensi Reproduksi Tikus Sprague Dawley Jantan. Laporan Kegiatan Penelitian FSM UNDIP. No.2173/UN.7.5.8.2/PP/2021.

Sitasiwi, Agung, J., S. Isdadiyanto, dan S. M. Mardiati. (2018). Effect of Ethanolic Neem (Azadirachta indica) Leaf Extract as an Herb Contraceptive on Hepatosomatic Index of the Male Mice (Mus musculus). Journal of Physics. Conference Series. 1-6.

Sukmayadi, A. E., S. A. Sumiwi, M. I. Barliana, and A. D. Aryanti. (2014). The Immunomodulatory Activity of Ethanol Extract of Tempuyung Leaves (Sonchus arvensis Linn.). Indonesian Journal of Pharmaceutical Science and Technology. 1(2): 65-72.

Swaroop, A. K., C. M. V. N. Lalitha, M. Shanmugam, G. Subramanian, J. Natarajan, and J. Selvaraj. (2022). Plant Derived Immunomodulators: A Critical Review. Advanced Pharmaceutical Bulletin. 12(4): 712-729.

Tasminatun, Sari, R. Pravitasari, N. Makiyah. (2017). Potential Ethanol of Carica papaya L. Extract as Immunomodulatory Through Histology Observation at Mice Balb/C Spleen. J Berkala Kedokteran. 13(2): 205-210.

Ulya, Lisana, H., A. Ismail, dan N. Susilaningsih. 2016. Pengaruh Pemberian Ekstrak Daun Piper crocatum Dosis Bertingkat terhadap Proliferasi Limfosit Limpa: Studi Pada Mencit Balb/c yang Diinfeksi Salmonella typhimurium. Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal). 5(4): 1192-1199.

Yanuhar, U., G. Hartawan, M. Musa, H. Suryanto, G. Mahasri, A. Supii, dan N. Caesar. (2023). Pengaruh Nanovaksin Berbasis Nanopartikel Hybrid Chitosan terhadap Sel Darah Ikan Kerapu Cantang Yang Diinfeksi VNN. Jurnal Ilmu Pertanian Indonesia. 28(4): 650-659.

DOI: https://doi.org/10.22146/jsv.92377