Deteksi Bakteri Patogen Pada Daging Ayam Broiler Sebagai Skrining Foodborne Diseases Di Kabupaten Jember
Enny Suswati(1*), Supangat Supangat(2), Alya Wafaul Lutfadaturroifa(3), Dava Rizky Pratama(4)
(1) Medical Faculty University of Jember
(2) 
(3) 
(4) 
(*) Corresponding Author
Abstract
Chicken meat is a source of animal protein that is very popular because of its high nutritional value, easy to obtain and cheap. However, as production increases, certain drugs and feed additives become essential for disease prevention, treatment, and growth promotion. The negative effect of using antimicrobial drugs is that they accumulate in animal tissues as residues and eventually become part of the food pyramid, subsequently impacting human health.
A total of 45 broiler chickens were purchased from broiler meat sellers in 9 traditional markets in Jember. We took five broiler chicken meat randomly from each market. Samples have identified the type of contaminant bacteria using Salmonella Chromogenic Agar and Mannitol Salt Agar. The results of this study showed that broiler chicken meat samples were contaminated with E. coli (45/45 (100%)), Staphylococcus aureus (43/45 (95.56%)), Salmonella spp. (38/45 (84.44%)) and Proteus spp. (14/45 (33.33%)). All broiler meat samples purchased at the Jember traditional market were contaminated with at least 1 type of bacteria, namely E. coli. The index of bacterial contamination is between 0.25 – 1. The most common contamination patterns are S. aureus, E. coli and Salmonella spp., with a bacterial contamination index of 0.75.
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Abdalrahman, L. S., Stanley, A., Wells, H., & Fakhr, M. K. (2015). Isolation, virulence, and antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA) strains from Oklahoma retail poultry meats. International Journal of Environmental Research and Public Health, 12(6), 6148–6161.
Akter, M., Sultana, S., & Munshi, S. (2019). Microbiological Quality Assessment of Ready-To-Eat Fried Chicken and Chicken Soup Samples Sold in Dhaka Metropolis , Bangladesh. Sumerianz Journal of Biotechnology, 2(7), 48–54.
Aung, K. T., Chau, M. L., Mak, K. W., Lim, N., Oh, J. Q., Kang, J. S. L., Lim, Y. H., Goh, T. L. V., Yap, H. M., Gutierrez, R. A., & Ng, L. C. (2018). Microbiological Assessment of Chicken Meat Sold At Chicken Rice Stalls in Singapore. Southeast Asian Journal of Tropical Medicine and Public Health, 49(6), 1043–1052.
Badan Standardidasi Nasional. (2000). Standar Nasional Indonesia No. 01-6366-2000 Batas maksimum cemaran mikroba dan batas maksimum residu dalam bahan makanan asal hewan. Journal of Chemical Information and Modeling, 1–12.
Bortolaia, V., Espinosa-Gongora, C., & Guardabassi, L. (2016). Human health risks associated with antimicrobial-resistant enterococci and Staphylococcus aureus on poultry meat. Clinical Microbiology and Infection, 22(2), 130–140.
BPS Kabupaten Jember. (2020). Kabupaten Jember Dalam Angka 2020. In Инновации.
CIVAS. (2021). Menjaga Keamanan Pangan Asal Unggas. 10.
Desokey, H., Edris, A. B., sabek, I., & Abd-ElGhaffar, A. (2020). Bacterial Status Of Broiler chicken Meat Meals Served at Governmental Hospital E. Benha Veterinary Medical Journal, 39(2), 153–157.
Effendi, M. H., Tyasningsih, W., Yurianti, Y. A., Rahmahani, J., Harijani, N., & Plumeriastuti, H. (2021). Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swabs of broilers in several wet markets in Surabaya, Indonesia. Biodiversitas, 22(1), 304–310.
Ghoddusi, A., Nayeri Fasaei, B., Zahraei Salehi, T., & Akbarein, H. (2019). Serotype Distribution and Antimicrobial Resistance of Salmonella Isolates in Human, Chicken, and Cattle in Iran. Archives of Razi Institute, 74(3), 259–266.
Haftay, A., Geberemedhin, H., Belay, A., Goytom, E., & Kidane, W. (2018). Antimicrobial resistance profile of Staphylococcus aureus isolated from raw cow milk and fresh fruit juice in Mekelle, Tigray, Ethiopia. Journal of Veterinary Medicine and Animal Health, 10(4), 106–113.
Hardiati, A., Safika, S., Wibawan, I. W. T., Indrawati, A., & Pasaribu, F. H. (2021). Isolation and detection of antibiotics resistance genes of Escherichia coli from broiler farms in Sukabumi, Indonesia. Journal of Advanced Veterinary and Animal Research, 8(1), 84–90.
Hennekinne, J.-A. (2018). Staphylococcus aureus as a Leading Cause of Foodborne Outbreaks Worldwide. In Staphylococcus aureus. Elsevier Inc.
Hermana, N. S. P., Nilasari, Z., Yuniarti, E., Riyanto, A., Safika, Indrawati, A., & Pasaribu, F. H. (2020). Antibiotic resistance profile of Staphylococcus aureus, Streptococcus spp. and Klebsiella spp. isolated form chicken farm in Bogor, Sukabumi, and Cianjur, West Java. Journal of Physics: Conference Series, 1430(1).
Hessel, C. T., de Oliveira Elias, S., Pessoa, J. P., Zanin, L. M., Stedefeldt, E., & Tondo, E. C. (2019). Food safety behavior and handling practices during purchase, preparation, storage and consumption of chicken meat and eggs. Food Research International, 125(May), 108631.
Jeong, J., Chon, J. W., Kim, H., Song, K. Y., & Seo, K. H. (2018). Risk assessment for salmonellosis in chicken in South Korea: The effect of Salmonella concentration in chicken at retail. Korean Journal for Food Science of Animal Resources, 38(5), 1043–1054.
Jung, Y., Porto-Fett, A. C. S., Shoyer, B. A., Henry, E., Shane, L. E., Osoria, M., & Luchansky, J. B. (2019). Prevalence, levels, and viability of Salmonella in and on raw chicken livers. Journal of Food Protection, 82(5), 834–843. https://doi.org/10.4315/0362-028X.JFP-18-430
Kayalvizhi, V., & Antony, U. (2011). Microbial a nd physico-chemical changes in tomato juice subjected to pulsed electric field treatment. African Journal of Agricultural Research, 6(30), 6348–6353.
Lee, S. K., Song, K. Y., Chon, J. W., Kim, D. H., & Seo, K. H. (2017). Evaluation of Selective-Enrichment and Chromogenic Media for Salmonella Detection in Raw Shell Egg Contents with a Low Microbial Load. Foodborne Pathogens and Disease, 14(7), 414–418.
Ma, L., Petersen, M., & Lu, X. (2020). Identification and antimicrobial susceptibility testing of campylobacter using a microfluidic lab-on-a-chip device. Applied and Environmental Microbiology, 86(9).
Mund, M. D., Khan, U. H., Tahir, U., Mustafa, B. E., & Fayyaz, A. (2017). Antimicrobial drug residues in poultry products and implications on public health: A review. International Journal of Food Properties, 20(7), 1433–1446.
Neunchat, P., Yangkao, K., Earm-sa-ard, N., Chatuphonprasert, S., Wiwattrakul, J., Manaijsin, J., Polruang, N., Sopa, A., Sukon, P., Kanistranon, K., Khaeng-air, S., & Noppon, B. (2017). Salmonella Prevalence, Antibiograms and Serotypes From Chicken Meat and Egg in Khon Kaen City, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 48(6), 1241–1247.
Nhung, N. T., Chansiripornchai, N., & Carrique-Mas, J. J. (2017). Antimicrobial resistance in bacterial poultry pathogens: A review. Frontiers in Veterinary Science, 4(AUG).
Novera, R., Rahayu, W. P., Kusumaningrum, H. D., Indrotristanto, N., & Nikastri, E. (2020). Prevalence of salmonella contamination in processing chain of selected chicken-based side dishes. Food Research, 4(3), 690–696.
OP, O., JO, I., PA, E., & SE, E. (2016). Proximate Analysis and Microbial Quality of Ready-To-Eat (RTE) Fried Chicken Parts. Journal of Food & Industrial Microbiology, 02(01).
Owuna, G., Nkene, I. H., & Joseph, G. W. (2015). Isolation and Antibiotic Susceptibility of Staphylococcus Aureus from Fresh Poultry Meat Sold in Keffi Metropolis , Nigeria. 3(11), 1–5. https://doi.org/10.13140/RG.2.2.23688.88328
Parry-Hanson Kunadu, A., Holmes, M., Miller, E. L., & Grant, A. J. (2018). Microbiological quality and antimicrobial resistance characterization of Salmonella spp. in fresh milk value chains in Ghana. International Journal of Food Microbiology, 277, 41–49. https://doi.org/10.1016/j.ijfoodmicro.2018.04.025
Perin, A. P., Martins, B. T. F., Barreiros, M. A. B., Yamatogi, R. S., Nero, L. A., & dos Santos Bersot, L. (2020). Occurrence, quantification, pulse types, and antimicrobial susceptibility of Salmonella sp. isolated from chicken meat in the state of Paraná, Brazil. Brazilian Journal of Microbiology, 51(1), 335–345. https://doi.org/10.1007/s42770-019-00188-x
Recto, J. B. P., Cabatic, K. J. A., Chua, G. A., Manamparam, A. A., Jalalon, K. C. B., & Romero, J. M. G. (2016). Detection of Salmonella Bacteria in Raw Chicken Meat Retailed at Ozamiz City Public Market. Journal of Multidisciplinary Studies, 5(1), 125–137.
Siddiky, N. A., Sarker, M. S., Khan, M. S. R., Begum, R., Kabir, M. E., Karim, M. R., Rahman, M. T., Mahmud, A., & Samad, M. A. (2021). Virulence and antimicrobial resistance profiles of salmonella enterica serovars isolated from chicken at wet markets in dhaka, bangladesh. Microorganisms, 9(5). https://doi.org/10.3390/microorganisms9050952
Sivaraman, S. (2018). Antibiotic Use in Food Animals: Indonesia Overview. ReAct Asia-Pacific, 1–31. https://www.reactgroup.org/wp-content/uploads/2018/11/Antibiotic_Use_in_Food_Animals_India_LIGHT_2018_web.pdforg
Tandi, J., Rimpa, M., & Tibe, F. (2018). Uji Efektivitas Antikolesteerol Ekstrak Etanol Daun Cincau Hijau Terhadap Tikus Putih Jantan Galur Wistar. Farmakologika Jurnal Farmasi, XV(2), 1–8.
Tang, Y., Larsen, J., Kjeldgaard, J., Andersen, P. S., Skov, R., & Ingmer, H. (2017). Methicillin-resistant and -susceptible Staphylococcus aureus from retail meat in Denmark. International Journal of Food Microbiology, 249, 72–76. h
Wibisono, F. J., Sumiarto, B., Untari, T., Effendi, M. H., Permatasari, D. A., & Witaningrum, A. M. (2020). CTX Gene of Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli on Broilers in Blitar, Indonesia. Systematic Reviews in Pharmacy, 11(7), 396–403.
World Health Organization (WHO). (2020). Food safety 30. Https://Www.Who.Int/En/News-Room/Fact-Sheets/Detail/Food-Safety.
Yin, M., Yang, B., Wu, Y., Wang, L., Wu, H., Zhang, T., & Tuohetaribayi, G. (2016). Prevalence and characterization of Salmonella enterica serovar in retail meats in market place in Uighur, Xinjiang, China. Food Control, 64, 165–172.
Yulistiani, R., Praseptiangga, D., Supyani, S., & Sudibya, S. (2019). Comparison of antibiotic resistance pattern among Enteropathogenic bacteria isolated from broiler and backyard chicken meat. Journal of the Indonesian Tropical Animal Agriculture, 44(2), 228–240.
Zhao, X., Lin, C. W., Wang, J., & Oh, D. H. (2014). Advances in rapid detection methods for foodborne pathogens. Journal of Microbiology and Biotechnology, 24(3), 297–312.
DOI: https://doi.org/10.22146/jsv.86084
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