Escherichia coli Surface Characters of Ettawah Cross Breed Goats Milk on the Adhession Ability of Mammary Epithelial Cells
Lalita Prasiddhanti(1*), A.E.T.H. Wahyuni(2)
(1) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(2) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Escherichia coli is a bacteria that may cause mastitis in goats. The ability of bacteria to infect is influenced by surface characters of each bacterial cell. Adhesion of bacteria on the udder epithelial cells plays an important role in the incidence of subclinical mastitis.The purpose of this study was to determine the surface characters of Escherichia coli colony morphology, such as the presence of polysaccharides capsule, hemagglutination activity
and hydrophobicity contributes to the adhesion process. Three isolates of Escherichia coli isolated from Ettawah cross breed goats on Mandiri and Pangestu Farm at Turi, Sleman, Yogyakarta were used in the study. The ability of E. coli to fermentize lactose was done by examining its ability in fermented lactose on eosin methylene blue. Hemagglutination ability was seen by hemagglutination test using sheep erythrocytes at concentrations of 0.5%, 1%, 1.5% and 2%, respectively. Hydrophobicity expression was done by salt
aggregation test using ammonium sulfate ([NH4] 2SO4) at concentrations of 1.2 M, 1.6 M, 2.0 M, 2.4 M and 3.2 M, respectively. The presence or absence of sera proteins was tested with sera soft agar, and the ability of adhesion was done by adhesion of Escherichia coli which has been marked by fluorescein isothiocyanate , with rats udder epithelial cells after birth. The results showed that isolates of E. coli with dry surface characters had a
thin polysaccharide capsule, positive hemagglutination , and hydrophobic, which had the ability to stick more on the udder epithelial cells after birth. Meanwhile, those with mucoid surface characters, had a thick polysaccharide capsule, negative hemagglutination, and hydrophilic which had a lower adhesion ability.
Keywords
Full Text:
PDFReferences
Daftar Pustaka
Bailey, W., Robert, ,Elvyn, G. and Scott, M. T.
(1962) Diagnostic Microbiology. Saint Louis.
The C. V. Mosby Company.
Baselga, R., Albizu, I., Cruz, M. D. L., Cacho, E.D.,
Barberan, M. and Amonera, B. (1993) Phase
v a r i a t i o n o f s l i m e p r o d u c t i o n i n
Staphylococcus aureus: Implication in
colonization and virulence. Infect. Immun. 61:
-4862.
Fernández-Garayzábal, F., Fernández, E., Las Heras,
A., Pascual, C., Collins, M.D. and Domínguez,
L. (1998) Streptococcus parasanguinis: New
pathogen associated with asymptomatic
mastitis in sheep. Universidad Complutense,
Madrid, Spain; and BBSRC Institute of Food
Research, Reading, United Kingdom.
Forsum, Urban, Forsgren, E. and Eva Hjelm, E.
(1972) Role of protein A in the serum-soft agar
technique. Infect. Immun. 6: 583-586.
Han, H-R., Pak, S-II., Kang, S-W., Jong, W-S. and
Youn, C-J. 2000. Capsular polissacharide
typing of domestic mastitis-causing
Staphylococcus aureus strains and its potensial
exploration of bovine mastitis vaccine
development. I. Capsular polisacharidae
typing, isolation and purrification of the strains.
J. Vet. Scien. 1: 53-60.
Jawetz, Melnick, and Adelberg's. (2001)
Mikrobiologi Kedokteran. Medika Salemba.
Kusnan and Siti Isrina Oktavia Salasia. 2006.
Respon Neutrofil, Adesi pada Sel Epitel,
Aglutinasi Eritrosit terhadap Staphylococcus
aureus: Kajian Hidrofobisitas in Vitro. J. S. V.
: 102-109.
Lalita Prasiddhanti dan A.E.T.H. Wahyuni
Lehninger, A. I. (1990) Dasar-Dasar Biokimia. Jilid
I ( d i t e r j e m a h k a n o l e h M a g g y
Thenawijaya).Penerbit Erlangga. Jakarta. 313.
Ljungh, A., Stellan, H. and Wadstorm, T. (1985)
H i g h s u r f a c e h y d r o p h o b i c i t y o f
autoaggregating Staphylococcus aureus strains
isolated from human infections studied with the
salt aggregation test. Infect. Immun. 47: 522-
Mather, J. P. and Robert, P.E. (1998) Introduction to
cell and tissue culture: Theory and technique.
Plenum Press. New York and London.
Quinn, P. J., Markey, B. K., Carter, M. E., Donnelly,
W. J. C. and Leonard, F. C. 2002. Veterinary
Microbiology and Microbiology Disease.
Blackwell Publishing, UK.
Rozgonyi, F., Szitha, K. R., Hjerten, S. and
Wadstorm, T (1991) Standardization of salt
aggregation test for reproducible determination
of cell-surface hydrophobicity with special
reference to Staphylococcus species. J. Appl.
Bacteriol. 59: 451-457.
Salasia, S. I. O. (2001) Perlekatan Streptococcus suis
pada permukaan sel-sel Hospes. Media Vet.. 8:
-51.
Wahyuni, A. E. T. H. (1998) Peran hemaglutinin
Streptococcus agalactiae dalam proses adesi
pada sel epitel sapi perah. Thesis Magister
Sains. Program Pasca Sarjana IPB, Bogor.
Wahyuni, A. E. T. H., Wibawan, I.W.T. dan
Wibowo, M.H. (2005) Karakterisasi
hemaglutinin Streptococcus agalactiae dan
Staphylococcus aureus penyebab mastitis
subklinis pada sapi perah. J.S.V. 23 (2).
Wibawan, I. W. T., Laemmler, Ch., and Pasaribu.
(1992) Role of hydrophobic surface protein in
mediating adherence of group B Streptococci to
epitelial cells. J. Gen.Microbiol. 138: 1237-
Wibawan, I. W. T., Eva, H., Damayanti, C. S. dan
Kammaludin, Z. (2005) Preparasi antiserum
terhadap hemaglutinin Streptococcus
agalactiae dan Staphylococcus aureus serta
perannya sebagai anti adhesin dan opsonin. J.
Vet. 6 (2).
DOI: https://doi.org/10.22146/jsv.8102
Article Metrics
Abstract views : 2979 | views : 8977Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats