Studi Reseptor Virus Infectious Bursal Disease (IBD) pada Organ Limfoid Ayam Pasca Vaksinasi dengan Metode Imunohistokimia

Restu Librani; Agus Setiyono; Wiwin Winarsih

doi:10.22146/jsv.48488

Studi Reseptor Virus Infectious Bursal Disease (IBD) pada Organ Limfoid Ayam Pasca Vaksinasi dengan Metode Imunohistokimia

https://doi.org/10.22146/jsv.48488

Restu Librani^(1*), Agus Setiyono⁽²⁾, Wiwin Winarsih⁽³⁾

(1) Fakultas Peternakan Universitas Halu Oleo
(2) Bagian Patologi, Departemen Klinik Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(3) Bagian Patologi, Departemen Klinik Reproduksi dan Patologi , Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(*) Corresponding Author

Abstract

Infectious bursal disease (IBD) affects economical impact for breeders due to it can cause damage lymphoid organ, especially bursa of Fabricius and causing failure the vaccination program. Infectious bursal disease virus receptors on lymphoid organs suspected contribute to subclinical and clinical IBD incidence in the chickens. The aim of this study was to determine and compare the distribution of IBDvirus receptors on lymphoid organ of the chicken which obtained different IBD vaccination program. The presence of virus receptors in bursa of Fabricius, spleen, and thymus were observed microscopically using immunohistochemical method and evaluated with Image J® software. Monoclonal anti LSCC-BK3 (Gifu University, Japan) antibodies as primary antibody was used in this study. The result showed that IBDvirus receptors found abudantly in the bursa of Fabricius, afterwards in the spleen and thymus. No significant differences of IBD virus receptor distribution within lymphoid organs between chicken which obtained once and twice IBD vaccination. Infectious bursal disease virus receptor distribution in bursa Fabricius chickens aged 23 days received twice IBD vaccination more than once IBD vaccination program.

Keywords

chickens; IBD; immunohistochemistry; receptor; vaccination

Full Text:

PDF

References

Aberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2002). Molecular Biology of the thCell 4 ed. Garland Science : New York. Besseboua, O., Ayad, A., Benbarek, H. (2015). Determination of the optimal time of vaccination againts infectious bursal disease virus (gumboro) in Algeria. J Vet Research. 82(1): 1-6.

Davison, F., Kaspers, B., Schat, K. A. (2008). Avian Immunology. Elsavier Ltd. USA. Enterradossi, N., Saif, Y. M. (2008). Disease of Poultry th: Infectious Bursal Disease 12 ed. Backwell : USA. Fantay, H., Balcha, E., Tesfay, A., Afera, B. (2015). Determining optimum time for administration of live intermediate vaccine of infectious bursal disease at Mekelle farm. J Vet Sci & Technol. 6: 223.

Gaballa, M. M. S., Soliman, M. A., Moustafa, S. A., Nasef, S. A., El-Mashad, Ab. I. (2016). Immunopathological cell tropism detection of infectious bursal disease in broiler chickens. Global Vet. 16(1): 86-92.

Gao, H., Qi, X. L., Li, K., Gao, L. H., Gao, Y. L., Qin, L. T., Wang, Y. Q., Wang, M. X. (2011). Development of a tailored vaccine againts challenge with very virulent infectious bursal disease virus of chickens using reverse genetics. Vaccine. 29: 5550-5557.

Hirai, K., dan Calnek, B. W. 1979. In vitro replication of infectious bursal disease virus in established lymphoid cell lines and chicken B lymphocytes. Infect. Immun. 25:964-970.

Homer, B L., Butcher, G. D., Miles, R. D., Rossi, A. F. (1992). Subclinical infectious bursal disease in an integrated broiler production operation. J vet Diagn Invest. 4: 406-411.

Kaufer, I., dan Weiss, E. 1976. Electron-microscope studies on the pathogenesis of infectious bursal disease after intrabursal application of the causal virus. Avian Dis. 20:483-495.

Kencana, G. A. Y., Adi, A. A. A. M., Ardana, I. B. K., Mahardika, I. G. N. K. (2011). Vaksin gumboro menyebabkan imunosupresif pada respon primer vaksin penyakit tetelo ayam pedaging. J Vet. 12(4): 275-280. Kim, I. J., Youn, S. K., Kim, H., Yeh, H. Y., Sharma, J. M. (2000). Characteristics of Bursal T Lymphocytes Induced by Infectious Bursal Disease Virus. J. of Virol. 74(19): 8884-8892.

Müller, H., Scholtissek, C., Becht, H. 1979. The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. J Virol 31:584–589 Muller, H., Islam, M. R., Raue, R. (2003). Research on infectious bursal disease-the past, the present, and the future. Vet Microbiol. 97: 153-165.

Niepper, H., Muller, H. (1996). Susceptibility of chicken lymphoid cells to infectious bursal disease virus does not correlate with the presence of specific binding sites. J. General virol. 77: 1229-1237.

Ogawa, M., Yamaguchi, T., Setiyono, A., Ho, T., Matsuda, H., Furusawa, S., Fukushi, H., Hirai, K. (1998). Some characteristics of cellular receptor for virulent infectious bursal disease virus by using flow cytometry. Arc Virol. 143: 2327-2341.

Setiyono, A., Hayashi, T., Yamaguchi, T., Fukushi, H., Hirai, K. (2000). Detection of cell membrane proteins interact with virulent infectious bursal disease virus. J. Vet. Med. Sci. 63(2): 219-221.

Setiyono, A., Yamaguchi, T., Ogawa, M., Fukushi, H., Hirai, K. (2001). Isolation of monoclonal antibodies that inhibit the binding of infectious

bursal disease virus to LSCC-BK3 cells. J Vet Med Sci. 63(2): 219-221.

Smith, J., Sadeyen, J. R., Butter, C., Kaiser, P., Burt, D. W. (2014). Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease. J.Virol. 89(5): 24692482. doi:10.1128/JVI.02828-14.

Tippehauer, M., Heller, D. E., Weigend, S., Rautenschlein, S. (2013). The host genotype influences infectious bursal disease virus pathogenesis in chickens by modulation of T cell responses and cytokine gene expression. DCI. 40: 1-10.

Uddin, M. M., Islam, M. S., Basu, J., Khan, M. Z. I. (2012). Distribution and quantification of lymphocytes in the major lymphoid organs of naturally gumboro infected broilers. Int. J. Morphol. 30(4): 1585-1589.

Van den Berg, T. P., Eterradossi, N., Toquin, D., Meulemans, G. (2000). Infectious bursal disease (Gumboro Disease). Rev Sci Tech. Off Int Epiz. 19(2): 527-543.

Wahyuwardani, S., Agungpryono, D. R., Parede, L., Manalu, W. (2011). Penyakit gumboro : etiologi, epidemiologi, patologi, diagnosis dan pengendaliannya. Wartazoa. 21(3).

DOI: https://doi.org/10.22146/jsv.48488