Aktivitas Antimikroorganisme Ekstrak Etanol 70 % Biji Bengkuang Terhadap Staphylococcus epidermidis, Pseudomonas aeruginosa dan Candida Albican
Min Rahminiwati(1*), Joshie Ramadhan(2), Oom Komala(3)
(1) Divisi Farmakologi dan Toksikologi Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(2) Divisi Farmakologi dan Toksikologi Departemen Anatomi, Fisiologi dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
(3) Fakultas MIPA Universitas Pakuan Bogor
(*) Corresponding Author
Abstract
Selain sebagai biopestisida ekstrak etanol 70 % biji bengkuang dilaporkan mempunyai aktivitas antibakteri terhadap bakteri gram positif Staphylococcus aureus dan bakteri gram negatif Escherichia coli dan Candida alibican. namun efeknya terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa, belum dketahui. Penelitian ini ditujukan untuk menentukan aktivitas antimikroorganisme ekstrak etanol 70 % biji bengkuang lokal terhadap Staphylococcus epidermidis, Pseudomonas aeruginosa dan Candida Albican menggunakan teknik difusi agar dan metoda dilusi. Aktivitas antimikroorganisme ekstrak diteliti pada konsentrasi 10% 12% 14%, 16% dan sebagai kontrol negatif, kontrol positif antibakteri dan kontrol positif antifungal masing masing digunakan akuadestilata. ertromisin 10 ppm, ketokonazol 10 ppm. Data yang diperoleh menunjukkan bahwa ekstrak efektif sebagai antibakteri terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa serta sebagai antifungi terhadap Candida albican, pada konsentrasi bunuh minimum 10 mg/ml.
Kata kunci: Ketokonazol, Eritromisin Pseudomonas biji bengkuang Candida albikan
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Bella, B.Y and Lynn, E.V. (1953). Examination of the Seed of Pachyrrhizus erosus. Journal of the American Pharmaceutical Association (Scientific ed.). 42 ( 2) : 117-118. Davis, W. W., dan Stout, T. R.( 1971). Disc Plate Method of Microbiological. Antibiotic Assay. Applied Microbiology. 22 (4): 659-665. Dep. Kes RI. (1977). Materia Medika Indonesia, Edisi III. Direktorat Pengawasan Obat dan Makanan. Jakarta. Dep. Kes RI. (1978). Materia Medika Indonesia. Jilid II. Jakarta. Direktorat Pengawasan Obat dan Makanan. Hal : 49-150. Dep. Kes RI. (1980). Materia Medika Indonesia. Jilid IV. Cetakan Pertama. Jakarta : Direktorat Jendral Pengawasan Obat dan Makanan. Dep. Kes RI. (1985). Cara Pembuatan Simplisia. Direktorat Pengawasan Obat dan Makanan. Jakarta. Hal : 1-22. Dep. Kes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jendral Pengawasan Obat dan Makanan.. Fransworth, N.R. (1966). Biological and Phytochemical Screening Of Plants. Journal od Pharmaceutical Sciences. 55 ( 3) : 262-264. Jadhav, V.J., and Pal, L. M. (2013). Human and domestic animal infections caused by Candida albicans. J. Mycopathol. Res. 51(2): 243—249. Jawetz, E. Melnick, J.L and Adelberg, E.A. 2001. Mikrobiologi Kedokteran. Edisi I. Salemba Medika. Jakarta. Hal 196-198. Juriah, J. (2003). Fraksinasi Ekstrak Biji Bengkuang (Pachyrrhizus erozus) yang Berpotensi Sebagai Antibakteri. Bogor : IPB. Khan, U.A., Rahman, H., Niaz, Z., Qassim, M., Khan, J., Tayyaba and Rehman, B. 2013. Antibacterial activity of Some Medicinal Plants Against Selected Human Pathogenic Bacteria, European Journal of Microbiology and Immunology 3 (2013) 4, pp. 272–274. Kumalasari, I.D., Nishi, K., Harmayani, E., Raharjo, S., Sugahara, T. (2014). Immunomodulatory Activity of Bengkoang (Pachyrhizus Erosus) Fiber Extract In Vitro And In Vivo. Cytotechnology. 66(1):75-85. Koneman, E.W., Allen, S.D,. Janda, W.M., Schreckenberger, P.C., and Winn, W.C. (1997). Color atlas and Textbook of Diagnostic Microbiology. Fifth Edition. Lippincott Williams & Wilkins, a Wolters Kluwer Company. Page : 171-199. Levinson, W.M.D., and Jawetz, E. (2003). Medical Microbiology And Immunology (Examination and Board Review). Seventh edition. McGraw Hill Companies. Nort America ; 286 – 288. McKane, L. and Kandel, J. (1996). Microbiology : Essentials and Applicationts. McGraw-Hill. New York. Mpila, D. A., Fatimawali., & Wiyono, W. I., (2012). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Mayana (Coleus atropurpureus [L] Benth) Terhadap Staphylococcus aureus, Eschericia coli, dan Pseudomonas aeruginosa secara In Vitro (online) (http://ejournal.unstrat.ac.id/index.php/pharmacon/article/view/440/351, diakses tanggal 10 Mei 2013). Necha, L.L.B., Baños, S.B., Luna, L.B., Suárez , F.J.L.G. (2004). Antifungal Activity of Seed Powders, Extracts, and Secondary Metabolites of Pachyrhizus erosus (L.) Urban (Fabaceae) Against Three Postharvest Fungi. Revista Mexicana de FITOPATOLOGIA, 22(3):356-361. Nines, K.R (1999). Inulin and Oligofruktosa what are they. Jurnal of Nutrition. 129 :1402S-1406 S. Parra, E.A.E., Verjana,J.C.G., Sa ´nchezab, I. G.L., Martı ´nez b,E.R.V., Castaneda, E.V., , Cerbon. M.A., Solano, D.A., and-Chilpaa.R.R. (2014) .Rotenone Isolated From Pachyrhizus Erosus Displays Cytotoxicity And Genotoxicity In K562 Cells .Natural Product Research. 28 ( 20): 1780–1785. Peng, B., Wang, C., Li,H., Su, Y.B., Ye, J.Y., Yang, M.J.M., and Peng. X.X. (2017). Outer Membrane Proteins form Specific Patterns in Antibiotic-Resistant Edwardsiella tarda . Front Microbiol.8: 69. Radji, M. (2011). Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi dan Kedokteran. Jakarta : Buku Kedokteran EGC. Hal 201-207. Robinson, T. (I995). Kandungan Organik Tumbuhan Tinggi. Bandung : ITB. Hal 132,157,191. Roberfroid, M.B. (2015) Prebiotic and Probiotic are They Functional Food. J.Clint. Nytr.18:117-143. Rubatzky. Vincent, E. dan Yamagutchy, M. (1998). Sayuran Dunia. Jilid ke-2. Bandung: ITB. Santos, A.C., Cavalcanti, M.S., Coelho, L.C.(1996). Chemical Composition And Nutritional Potential Of Yam Bean Seeds (Pachyrhizus Erosus L. Urban). Plant Foods Hum Nutr. 49(1):35-41. Wiredu, C.B. 2014. Evaluation of Starch properties, Phytochemical Composition and Antimicrobial Activities of Yam Bean (Pachyrhizus erosus (L Urban.). Thesis Kwame Nkrumah University of Science and Technology. Wesley, A.V., dan Margaret, F.W. (1989). Mikrobiologi Dasar. Edisi 5 jilid II. LIPI. Jakarta: Erlangga. Hal 155,156,157,195,196. Woodford, N., Turton, J..F., and Livermore, D.M. (2011). Multiresistant Grambnegative Bacteria: the Role of High-Risk Clones In The Dissemination Of Antibiotic Resistance. FEMS Microbiol Rev.35(5): 736-55. https://doi.org/10.1111/j.1574-6976.2011.00268.x PMid:21303394. Zavascki, A.P., Zavascki, A.P., Carvalhaes, C.G., Picão ,R.C., Gales, A.C. (2010). Multidrug-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii: Resistance Mechanisms and Implications for Therapy. Expert Rev Anti Infect Ther. 8(1):7193. https://doi.org/10.1586/eri.09.108 PMid:20014903. Zowalaty,M.E., Al Thani, A.A., Webster,T.J., El Zowalaty,A.E., Schweizer,H.P., Nasrallah, G.K., Marei, H.E., Ashour, H.M. (2015) Pseudomonas aeruginosa: arsenal of resistance mechanisms, decades of changing resistance profiles, and future antimicrobial therapies. Future Microbiol.10 (10): 1683-706. https://doi.org/10.2217/fmb.15.48 PMid:26439366
DOI: https://doi.org/10.22146/jsv.44589
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