Evaluasi Pemberian Obat Diminazene Aceturate Secara In Vivo Pada Mencit (Mus musculus) yang Diinfeksi Isolat Trypanosoma evansi
Reza Yesica(1*), Bambang Sutrisno(2), Wisnu Nurcahyo(3)
(1) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(2) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(3) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Abstract
Surra's disease is caused by Trypanosoma evansi parasite has been established as one of the strategic infectious animal diseases. Drug resistance in this case is one of the major challenges in handle and control them. The aim of this study is to evaluate the provision drug resistance diminazene aceturate (Tryponil®) on Trypanosoma evansi isolate from Pemalang and Brebes Central Java province with in vivo test in mice. Total 50 mice, BALB / c strain, male, 2 months, body weight ± 30 gram are obtained from LPPT-UGM, adapted for one week. Mice were divided into 10 groups consist of 5 each. Each mouse was infected with Trypanosoma evansi by intraperitonial route. Treatment was given when mice had reached the level of parasitemia 108 – 109 trypanosoma / mL of blood this was predicted 24 hours post-infection (Eisler et al., 2001). The administration of the drug tryapanosidal was done intraperitonial with doses 1mg/kg, 3mg / kg, 5 mg / kg and 7mg / kg. Observation of parasitemia did every 2 times in one week till 60 days of observation. Parasitemia observation was performed using 3 techniques. The first method was native examination used a microscope, if the negative results would be followed by MHCT (Microhaematocrit centrifugation Technique) and BCT (Buffy Coat Technique) according to OIE (2012). Data obtained from the treatment group were the level of parasitemia, the number of deaths and the number of live mice from each test dose. The results are analysed by standard logit or probit. The results of this study showed the effects of the drug Dimianzene aceturate on both isolates varied. On Brebes Isolate was effective at doses of 7 mg / kg BW (100%) and 5mg / kg BW (80%), whereas in the effective dose Pemalang isolate at 3 mg dose / kg BW (80%), 5 and 7 mg / kg BW (100%). While at the lowest dose of 1 mg / kg obtained a level of effectiveness of 0% in both isolates. It could be concluded that both isolates have different pathogens and indicate resistance subpopulation to diminazene aceturate.
Keywords : diminazene aceturate, in vivo, resistance, Trypanosoma evansi.
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Anene, B.M., Onah D.N., Nawa Y. Drug resistance in pathogenic African trypanosoma what hopes for the future?. Vet Parasitol 2001; 96: 83-10. Boid, R., Jones, T.W., Payne, R.C., 1989. Malic enzyme type VII isoenzyme as an indicator of suramin resistance in Trypanosoma evansi. Exp. Parasitol. 69, 317–323. Bruning, A., 1996. Equine piroplasmosis: an update on diagnosis, treatment and prevention. Br. Vet. J. 152, 139–151. Davison, H.C., Thrusfield M.V., Husein A., Muharsini S., Partoutomo S., Rae P., Luckins A.G. 2000. The occurrence of Trypanosoma evansi in buffaloes in Indonesia, estimated using various diagnostic tests. Epidemiol Infect. 124:163-172. Desquesnes, M., Dargantes A., Lai D.H., Lun Z.R., Holzmuller P., Jittapalapong S. 2013. Trypanosoma evansi and Surra: a review and perspectives on transmission, epidemiology and control, impact and zoonotic aspects. Biomed Res Int. 2013:1-20. Dieleman, E.F., 1986. Trypanosomiasis in Indonesia. Vet. Q. 8, 250–256. Eisler, M.C., Brandt J., Bauer B., Clausen P.H., Delespaux V., Holmes P.H., Ilemobade A., Machila N., Mbwambo H., McDermott J., Mehlitz D., Murilla G., Ndung’u J.M., Peregrine A.S., Sidibé I., Sinyangwe L., Geerts S. 2001. Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle. Vet Parasitol. 97:171-183. El Rayah, I.E., Kaminsky, R., Schmid, C., El Malik, K.H., 1999. Drug resistance in Sudanese Trypanosoma evansi. Vet. Parasitol. 80, 281–287. Gillingwater, K., Büscher P., Brun R. 2007. Establishment of a panel of reference Trypanosoma evansi and Trypanosoma equiperdum strains for drug screening. Vet Parasitol. 148:114-121. Kroubi M, Karembe H, Betbeder D. 2011. Drug delivery systems in the treatment of African trypanosomiasis infections. Exprt Opin Drug Deliv 8(6): 735-47. Lazuardi, M., Wahyudi M.T., Munier R.S. 2013. Re-evaluate interrelathionship dose-response of Diminazene aceturate against to infected mice of Trypanosoma evansi Bangkalan isolate. Jurnal Vteriner Juni 2013. Vol 14.No.2: 173-177. Luscher, A. (2006) Combined contribution of TbAT1 and TbMRPA to drug resistance in Trypanosoma brucei. Mol. Biochem. Parasitol. 150, 364–366. Macaraeg, B.B., Lazaro J.V., Abes N.S., Mingala C.N. 2013. Invivo assessment of the effects of trypanocidal drugs against Trypanosoma evansi isolates from Philippine water buffaloes (Bubalus bubalis). Vet Arh. 83:381392. Nurcahyo, R. W. 2015. Pengembangan metode diagnosa molekuler Trypanosomiasis pada sapi dan kerbau sebagai upaya penanggulangan penyakit Surra di Indonesia. Laporan Kemajuan Hibah Strategi Nasional. Kementerian Riset, Teknologi dan Pendidikan Tinggi. [OIE] Office International des Epizooties. 2012. Trypanosoma evansi Infection (Surra). OIE Terrestrial Manual 2012, Chapter 2.1.17. Paris (France): Office International des Epizooties. p. 1-15. Peregrine A.S and Mamman M. 1991. Pharmacology of diminazene aceturate: a review. Elsevier Science Publishers. 1993: 185-203. Ronohardjo, P., Wilson A.J., Partoutomo S. and Hirst R.G. 1986. Some aspects of the epidemiology and economics of important diseases of large ruminants in Indonesia. Di dalam Davison HC, Thrusfield MV, Husein A, Muharsini S, Partoutomo S, Rae P and Luckins AG, editor. 2000. The occurrence of Trypanosoma evansi in buffaloes in Indonesia, estimated using various diagnostic tests. Epidemiol. Infect. 124 : 163 – 172. Subekti, D.T., Yuniarto I., Sulinawati, Susiani H., Santosa B., Amaliah F., Utomo B.N., Dahlan M., Suharyanto dan Sukarya. 2015. Perbedaan Kepekaan Antar Isolat Trypanosoma Indonesia Terhadap Beberapa Trypanosidal. JITV in press. Sukanto, I.P., Payne R.C.,Graydon R. 1988. Trypanosomiasis di Madura: Survei Parasitologik dan Serologik. Penyakit Hewan 19(13): 14-16.
DOI: https://doi.org/10.22146/jsv.43100
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