Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus  Isolat Preputium Sapi Aceh  (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate)

Maryulia Dewi; Darmawi Darmawi; Nurliana Nurliana; T. Fadrial Karmil; T Zahrial Helmi; Fakhrurrazi Fakhrurrazi; Erina Erina; Mahdi Abrar; M. Daud AK; Masda Admi

doi:10.22146/jsv.35219

Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate)

https://doi.org/10.22146/jsv.35219

Maryulia Dewi^(1*), Darmawi Darmawi⁽²⁾, Nurliana Nurliana⁽³⁾, T. Fadrial Karmil⁽⁴⁾, T Zahrial Helmi⁽⁵⁾, Fakhrurrazi Fakhrurrazi⁽⁶⁾, Erina Erina⁽⁷⁾, Mahdi Abrar⁽⁸⁾, M. Daud AK⁽⁹⁾, Masda Admi⁽¹⁰⁾

(1) Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(2) Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(3) Program Studi Magister Kesmavet Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(4) Program Studi Magister Kesmavet Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(5) Laboratorium Biokimia Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(6) Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(7) Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(8) Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(9) Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(10) Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh, Aceh
(*) Corresponding Author

Abstract

Abstract

Biofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium were used to biofilm. Bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive.

Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh.

Abstrak

Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu.

Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh.

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References

Daftar Pusaka Ardani, M., Sylvia, U.T.P., dan Triana, H. 2010. Efek campuran minyak atsiri daun cengkeh dan kulit batang kayu manis sebagai antiplak gigi. Farmasi Indonesia, 21 (3): 191–201. Bjarnsholt, Th., Jensen, P.O., Moser, C., and Hoiby, N. (2011). Biofilm Infections. Springer-Verlag. New York: 1014. Choon, K. K., Catarina, M., Hermínia, L., and Alexander, T. (2017). Antibiotic resistance as a stress response: Recovery of high-level oxacillin resistance in methicillin-resistant Staphylococcus aureus “auxiliary” (fem) mutants by Induction of the stringent stress response. J Antimicrob Chemother. 61(8): e00313-17. Das, S.C. and K.N. Kapoor. (2004). Efect of growth medium on hydrophobicity of Staphylococcus epidermidis. Indian J. Med. Res. (119): 107 – 109. Hoiby, N., Bjarnsholt, T., Giuskov, M., Molin, S., and Ciofu, O. 2011. Antibiotic resistance of bacterial biofilm. J. Antimicrobial Agents 34 (4): 322-325. Homenta, H. (2016). Infeksi biofilm bakterial. Jurnal e-Biomedik 1 (4): 1-11. Karmakar, A., Dua, P., and Ghosh, C. 2016. Biochemical and molecular analysis of Staphylococcus aureus clinical isolates from hospitalized patients. Hindawi Publishing Corporation. Canadian Journal of Infectious Diseases and Medical Microbiology. (1): 1-7. Lahkar, V., Saika, L.,Saura, J.P., Nath, R., and Das,P.P. (2017). Estimation of biofilm, proteinase & phospholipase production of the Candida species isolated from the oropharyngeal samples in HIV-infected patients. Indian J Med Res (145): 635-640. Lestari, F. B., dan Siti, I.O.S. 2015. Karakterisasi Staphylococcus aureus isolat susu sapi perah berdasarkan keberadaan protein-A pada media serum soft agar terhadap aktivitas fagositosis secara invitro. JSV 33 (20): 149-155. Ma’rufah, 2007. Keanekaragaman bakteri yang terdapat dalam preputium pejantan sapi unggul di Balai Besar Inseminasi Buatan (BBIB) Singosari Malang. Skripsi. Unversitas Negeri Malang. Merle, E. O., Ceri, H., Douglas, W., Morck, A.G., Buret, and R. R. Read. (2002). Biofilm bacteria: formation and comparative susceptibility to antibiotics, Canadian J Veterinary Res. 66(2): 86–92. Monroe, D. (2007). Looking for chinks in the armor of bacterial biofilms. J PLoS Biol. 5(11): 307. Moretti, E., Capitani, S., Figura, N., Pammolli, A., Federico, M.G., Giannerini, V., and Collodel G. (2009). The presence of bacteria species in semen and sperm quality. J Assist Reprod Genet. 26 (1): 47–56. Nicolic, M., Vasic, S., Durdevic, J., Stevanovic, O., and Comic, L. (2014). Antibacterial and anti-biofilm activity of ginger (Zingiber officinate (Roscoe)) ethanolic extract. Laboratory of Microbiology, Department of Biology and Ecology, Faculty of Science, University of Kragujevac. Kragujevac J. Sci. (36) 129-136. Prawesthirini, S., Ferianto, A., dan Supranianondo, K. (2012). Pola resistensi Staphylococcus aureus yang diisolasi dari mastitis pada sapi perah di wilayah kerja KUD Argopuro Krucil Probolinggo terhadap antibiotika. J Vet Medika. 5 (3): 181-186. Riddle, L. K. 2001. Of biofilm resistance antimicroba agents chemotherapy. J Med Microbiol. (45): 999-1007. Sader, H.S., Fey, P.D., Fish, D.N., Limaye, A.P., Pankey, G., Rahal, J., Rybak, M.J., Snydman, D.R., Steed, L.L., Waites, K., and Jones, R.N. (2009). Evaluation of vancomycin and daptomycin potency trends (MIC creep) against methicillin-resistant Staphylococcus aureus isolates collected in nine U.S. medical centers from 2002 to 2006. J Antimicrob Chemother. 53(10): 4127-32. Sarah, T., Aurélie, C., Mayram, H., Liesbeth, S., Silke, H., Petra, R., Inne, D., and Tom, C. (2017). Community composition determines activity of antibiotics against multispecies biofilms. J Antimicrob Chemother. 61(9): e00302-17. Singh, J., Murray, R.D., Mshelia, G., and Woldehiwet, Z. (2008). The immune status of the bovine uterus during the peripartum period. The Veterinary Journal 175 (3) : 301-309.

DOI: https://doi.org/10.22146/jsv.35219