Kualitas Oosit Kerbau dari Status Reproduksi Ovarium yang Berlainan

Sri Gustina; Hasbi Hasbi; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi

doi:10.22146/jsv.34695

Kualitas Oosit Kerbau dari Status Reproduksi Ovarium yang Berlainan

https://doi.org/10.22146/jsv.34695

Sri Gustina^(1*), Hasbi Hasbi⁽²⁾, Ni Wayan Kurniani Karja⁽³⁾, Mohamad Agus Setiadi⁽⁴⁾

(1) Mahasiswa Program Doktor, Sekolah Pascasarjana, Institut Pertanian Bogor, Jl. Agatis, Kampus IPB Darmaga, Bogor Jurusan Peternakan, Fakultas Peternakan dan Perikanan, Universitas Sulawesi Barat, Jl. Prof. Dr. Baharuddin Lopa, Tande Timur, Majene, 91412
(2) Mahasiswa Program Doktor, Sekolah Pascasarjana, Institut Pertanian Bogor, Jl. Agatis, Kampus IPB Darmaga, Bogor Jurusan Produksi ternak, Fakultas Peternakan, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Makassar
(3) Divisi Reproduksi dan Kebidanan, Departemen Klinik, Reproduksi, dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis, Kampus IPB Darmaga, Bogor
(4) Divisi Reproduksi dan Kebidanan, Departemen Klinik, Reproduksi, dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis, Kampus IPB Darmaga, Bogor
(*) Corresponding Author

Abstract

In vitro embryo production relies on the quality of oocytes, however the quality is subjected to ovaries reproduction cycle. This study was conducted to observe the potency of buffalo ovaries from various reproductive cycle in producing quality oocytes. Collected pairs of ovaries from slaughter house were weighed and grouped of 5 according to the cycle. Oocytes were collected by slicing techniques, then selected based on quality. The results showed the average weight of buffalo ovaries of (+CL, +FD); (+CL, -FD); (-CL, +FD); (-CL, -FD) are 7.2 g; 5.5 g; 4.1 g; and 4.5 g respectively. No significant quality difference of produced oocytes between ovaries cycles (P>0.5). Good quality of collected oocytes were only 40-55%. Approximately 2-5 oocytes of grade A and 1-5 oocytes of grade B can be collected per pair of ovaries.

Keywords

reproduction cycle; buffalo ovaries; oocytes quality; status reproduksi; ovarium kerbau; kualitas oosit

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DOI: https://doi.org/10.22146/jsv.34695