Efektivitas Ekstrak Biji Kapas (Gossypium hirsutum L.) terhadap Jumlah dan Viabilitas Embrio Mencit (Mus musculus L.)

https://doi.org/10.22146/jsv.27563

Nofri Zayani(1*), Iman Supriatna(2), Mohamad Agus Setiadi(3)

(1) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(2) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(3) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(*) Corresponding Author

Abstract


The cottonseed (Gossypium hirsutum L.) contained gossypol as antifertility agent. The effect of cottonseed extract treatment could be decrease and impaired follicles development were accompanied by oocytes
damage. Damage of oocytes resulted reduction of number and viability of embryos. The objective of this study was to evaluate the effectiveness of cottonseed extract (Gossypium hirsutum L.) on the number and viability of
mice embryo (Mus musculus L.). Doses of the cottonseed extract were used consists of 0 (control), 1.5; 2.1; and 2.7 g/kg of body weight (BW) for 24 days via the oral route. This research used 24 animals healthy of female
DDY mice 14-15 weeks old and 30-35 g BW. Embryos were collected at day 4 of pregnancy by flushing the utery cornua. The collected embryos were cultured in vitro for 48 hours in modified phosphate buffered saline (PBS) supplemented with 10% fetal bovine serum (FBS) culture medium according to the stage of its development to observe the viability of embryos. The result showed that the cottonseed extract with doses 1.5; 2.1; and 2.7 g/kg of body weight (BW) made the number of embryos which collected in D4 of pregnancy significantly lower
than control (P<0.05). Data from embryos culture in vitro for 48 hours decreased embryos number (P<0.05) that developed in to the expanded and hatched blastocysts. At 2.7 g/kg BW, embryos only can develop to the
blastocysts stage. Retardation (4-8 cells) and degeneration embryos did not develop in culture


Keywords


embryo numbers; gossypol; viability of mice embry

Full Text:

PDF


References

AVMA [American Veterinary Medical Association].(2013). Guidelines for the Euthanasia of Animals. Penerbit Meacham Road Pr.

Schaumburg: 38-39.

Bai, J. and Shi Y. (2002). Inhibition of T-type Ca2+

currents in mouse spermatogenic cells by

gossypol, an antifertility compound. Eur. J.

Pharmacology. 440:1-6.

Basini, G., Bussolati, S., Baioni, L. and Grasselli, F.

(2009). Gossypol, a polyphenolic aldehyde from

cotton plant interferes with swine granulosa cell

function. Dom. Anim. Endocrinol.37:30-36.

Brocas, C., Rivera, M. R., Paula-Lopes, F. F., McDowell,

L. R., Calhoun, M. C., Staples, C. R., Wilkinson,

N. S., Boning, A. J., Chenoweth, P. J. and Hansen,

P. J. (1997). Deleterious actions of gossypol on

bovine spermatozoa, oocytes, and embryos. Biol.

Reprod. 57:901-907.

Camara, A. C. L., Gadelha, I. C. N., Borges, P. A. C.,

Paiva, S. A., Melo, M. M. and Blanco, B. S.

(2015). Toxicity of gossypol from cottonseed

cake to sheep ovarian follicles. Research

Article Plos One. 10:1-11.

Cecconi, S. (2002). Growth and differentiation of small

ovarian follicles in mammals: problems and

future perspective. Reprod. Dev. 48: 431-445.

Cheng, J. S., Lo, K. Y., Yeh, J. H., Cheng, H. H., Liu,

C. P., Chen, W. C. and Jan, C. R. (2003). Effect

of gossypol on intracellelar Ca2+ regulation in

human hepatoma cells. Chin. J. Physiol. 46:

-122.

Gadelha, I. C. N., Fonsesa, N. B. S., Oloris, S. C.

S., Melo, M. M. and Blanco, S. B. (2014a).

Gossypol toxicity from cottonseed products.

Sci. World. 14:1-9.

Gadelha, I. C. N., deMacedo, M. F., Melo, M. M.and

Blanco, B. B. (2014b). Gossypol promotes

degeneration of ovarian follicles in rats. Sci.

Word. 14: 1-7.

Galvao, K. N., Santos, J. E. P., Coscioni, A. C.,

Juchem, S. O., Chebel, R. C., Sischo, W. M.

and Villasenor, M. (2006). Embryo survival

from gossypol feed heifers after transfer to

lactating cows treated with human chorionic

gonadotropin. Dairy. Sci. 23:123-131.

Gordon, I. (2003). Laboratory Production of Cattle

Embryos 2rd ed. Penerbit CABI Publishing.

Willingford. 225-226.

Gu, Y., Lin, Y. C. and Rikihisa, Y. (1991). Inhibitory

effect of gossypol on steriodogenic pathways

in cultured bovine luteal cells. Biochem.

Biophysic. Res. Com. 61:169-179.

Hernandez, E. (2016). Cottonseed. Article Food. Sci.

:1-5.

Herve, J. C., Bastide, F. P. B., Cronier, L., Verrecchia,

F., Malassine, A. and Joffre, M. (1996).

Contraceptive gossypol blocks cell to cell

communication in human and rat cells. Eur. J

Pharm. 313: 242-255.

Kovacic, P. (2003). Mechanism of drug and toxic

actions of gossypol: focus on reactive oxygen

species and electron transfer. J. Curr. Medic.

Chem. 10: 2711-2718.

Kurniawati, H. (2010). Hubungan pemakaian

kontrasepsi pil KB kombinasi dengan tekanan

darah tinggi pada wanita pasangan usia subur

di Puskesmas Kecamatan Grogol Petamburan

Kota Administrasi Jakarta Barat tahun 2010.

Tesis. Fakultas Kesehatan Masyarakat,

Universitas Indonesia, Depok.

Lin, Y. C., Gu, Y., Brueggemeier, R. W. and Rikihisa,

Y. (1992). Binding of 3H-Gossypol in

organelles of cultured bovine luteal cells. Life.

Sci. 50:443-447.

Lin, Y. C., Sanbuissho, A., Coskun and Rikihisa, Y.

(1994). Inhibition of in vitro fertilization and

early embryonic development in hamsters by

gossypol. Life Sci. 55:14-19.

Lonergan, P., Rizos, D., Ward, F. and Boland, M. P.

(2001). Factors influencing oocyte and embryo

quality in cattle. Reprod. Nutr. Dev. 41: 427-437.

Nawrot, T. S., Hond, E. D., Fagard, R. H.,

Hoppenbrouwers, K. and Staessen, J. A. (2003).

Blood pressure, serum total cholesterol, and

contraceptive pill use in 17 year old girls. Eur

Cardiovasc Pretention Rehab. 10:438-442.

Nasim, A., Schrick, F. N., Butcher, R. L. and Inskeep,

E. K. (1995). Effect of persistent follicles

on early embryonic losses in beef cow. Biol

Reprod. 52:1129-1135.

Neganova, I. E. G. G., Sekirina and Ritter, U. E.

(2000). Surface-expressed E-cadherin, and

mitochondrial and microtubule distribution in

rescue of mouse embryos from 2-cell block by

aggregation. Mol. Hum. Reprod. 6: 454-46.

Pan, L. C., Nadakavukaren, M. J. and Jensen, D.

R. (1987). Effect of ingested gossypol on the

ultrastucture of rat ovarian follicles. Cell. Tiss.

Res. 250:215-220.

Ramadhani, S. A. (2016). Pengendalian folikulogenesis

ovarium mencit (Mus musculus) dengan pemberian

ekstrak biji kapas (Gossypium hirsutum).

Tesis. Institut Pertanian Bogor. Bogor: 24.

Rusmiati. (2010). Pengaruh ekstrak methanol kulit

kayu durian (Durio zibethinus Murr) pada

struktur mikroanatomi ovarium dan uterus

mencit (Mus musculus L.) betina. Sains Terap

Kim. 4: 29-37.

Sahid, M.dan Wahyuni, S. A. (2001). Kapas: Keragaan

dan Konsep Perbaikan Pengembangan Kapas

Di Indonesia. Penerbit Balittas. Malang: 1-16.

Said, S., Astirin, O. P. dan Wahyuningsih, S. (2011).

Tingkat fertilisasi dan perkembangan embrio

mencit yang diberi ekstrak buah merah. Med

Petern. 34: 112-116.

Sirard, M. A., Richard, F. O., Blondin, P. And Robert,

C. (2006). Contribution of the oocyte to

embryo quality. Theriogenology. 65:126-136.

Supriatna, I., Tolihere, M. R., Yusuf , T. L. dan

Purwantara, B. (1993). Pengaruh penambahan

fetal serum, calf serum, dan bovine serum

dalam pupukan in vitro terhadap viabilitas

embrio mencit. Laporan Penelitian. Institut

Pertanian Bogor. Bogor: 23

Theiler, K. (1989). The House Mouse:Atlas of

Embryonic Development. Penerbit Springer

Verlag Inc. Switzerland: 2-12.

Velasquiez-Pereira, J., Risco, C. A., McDowell, L.

R., Staples, C. R., Prichard, D., Chenoweth, P.

J., Martin, F. G., Williams, S. N., Rojas, L. X.,

Calhoun, M. C. and Wilkinson, N. S. (2002).

Long term effect of feeding gossypol and

vitamin E to dairy calves. Dairy Sci.82:1240-1251.

Villasenor, M., Coscioni, A. C., Galvao, K. N.,

Chebel, R. C. and Santos, J. E. P. (2008).

Gossypol distrupt embryos development in

Heifers. Dairy. Sci. 91: 3015-3024.

Yuan, Y. Y. and Shi, Q. X. (2000). Inhibition of

hamster sperm acrosomal enzyme by gossypol

is closely associated with the decrease in

fertilization capacity. Contraception. 62: 203-209.



DOI: https://doi.org/10.22146/jsv.27563

Article Metrics

Abstract views : 2843 | views : 5851

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats