Deteksi Spesies Brucella pada Kambing di Rumah Potong Hewan Jakarta

https://doi.org/10.22146/jsv.27546

Mujiatun Mujiatun(1*), Retno Damajanti Soejoedono(2), Etih Sudarnika(3), Susan Maphilindawati Noor(4)

(1) Balai Besar Uji Standar Karantina Pertanian, Bandan Karantina Pertanian
(2) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(3) Balai Besar Penelitian Veteriner -Bogor, Badan Penelitian dan Pengembangan Pertanian Bogor
(4) 3Balai Besar Penelitian Veteriner -Bogor, Badan Penelitian dan Pengembangan Pertanian, Bogor
(*) Corresponding Author

Abstract


Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected from
slaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and Polymerase
Chain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limph
and limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.
The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown.


Keywords


Brucella spp.; goat; zoonosis; slaughterhouse

Full Text:

PDF


References

Alton, G. (1990). Brucella melitensis. In K. Nielsen

and J. Duncan (Eds.), Animal Brucellosis (pp.

–409). Boca Raton, Florida: CRC Press.

Alton, G.G., Jones, L.M., Angus, R.D. V.2. (1988).

Techniques for the Brucellosis Laboratory.

Mujiatun et al.

Paris: Institut National de la Recherche

Agronomique. INRA Press.

Aworh, M. K., Okolocha, E., Kwaga, J., Fasina, F.,

Lazarus, D., Suleman, I., Subuga, P. (2013).

Human brucellosis: seroprevalence and

associated exposure factors among abattoir

workers in Abuja, Nigeria - 2011, 8688, 1–9.

Batson, M. (2006). Step PCR Assay for identification

of classical Brucella strains (pp. 1–9). CSIRO

(AUS): Australian Animal Health (AAHL).

Blasco, J. M. (1992). Diagnosis of Brucella

melitensis infection in small ruminants. In M.

Plommet (Ed.), Prevention of Brucellosis in

the Mediterranean Countries (pp. 272–277).

JOUR, Wegeningen: Pudoc Scientific.

Blasco, J. M., Garin-Bastuji, B., Marin, C. M.,

Gerbier, G., Fanlo, J., Jiménez de Bagués, M. P.,

and Cau, C. (1994). Efficacy of different Rose

Bengal and complement fixation antigens for

the diagnosis of Brucella melitensis infection

in sheep and goats. The Veterinary Record.

https://doi.org/10.1136/vr.134.16.415.

Celli, J., Chastellier, C. De, Franchini, D., Pizarrocerda,

J., Moreno, E., and Gorvel, J. (2003).

Brucella Evades Macrophage Killing via

VirB-dependent Sustained Interactions with

the Endoplasmic Reticulum, 198(4). https://

doi.org/10.1084/jem.20030088.

EC [European Commission]. (2001). Brucellosis

in Sheep and Goats. Scientific Committe on

Animal Health and Animal Welfare, 1–20.

Farina, R. (1985). Current serological methods in B.

melitensis diagnosis. In : Brucella melitensis.

(Plommet, M., Verger, J. M., eds), Martinus

Nijhoff Publ., Dordrecht, 139-146.

Ficht, T. A. (2003). Intracellular survival of Brucella:

Defining the link with persistence. Veterinary

Microbiology, 92(3),213–223. https://doi.org/

1016/S0378-1135(02)00367-X.

Gupta, V. K., Nayakwadi, S., Kumar, A., Gururaj, K.,

Kumar, A., and Pawaiya, R. S. (2014). Markers

for the molecular diagnosis of brucellosis in

animals. Adv. Anim. Vet. Sci, 2, 31–39.

Harlow E and Lane, D. (1988). Antibodies A

Laboratory Manual. Cold Spring Harbor

Laboratory. New York.

John, W., Cherwonogrodzky, Dubray, G., Moreno, E.,

M. H. (1990). Antigens of Brucella. In: Animal

Brucellosis. (J. R. Nielsen, K., Duncan, Ed.).

Boca Raton: and 5 and Press Inc.

MacMillan, A. 1990. Conventional Serological Tests.

In: Animal Brucellosis. (Nielsen, K., Duncan,

J.R., eds). CRC Press Inc., Boca Raton, pp.

-198.

Nabukenya, I., Kaddu-mulindwa, D., and Nasinyama,

G. W. (2013). Survey of Brucella infection and

malaria among Abattoir workers in Kampala

and Mbarara Districts , Uganda. BMC Public

Health, 13(1), 1. https://doi.org/10.1186/1471-

-13-901.

Nasinyama, G., Ssekawojwa, E., Opuda, J., Grimaud,

P., Etter, E., and Bellinguez, A. (2014).

Brucella sero-prevalence and modifiable risk

factors among predisposed cattle keepers and

consumers of un-pasteurized milk in Mbarara

and Kampala districts, Uganda Abstract :,

(4), 2–5.

OIE. (2009). Bovine Brucellosis. OIE Terrestrial

Manual 2009, (May), 1–35. https://doi.

org/10.1016/j.cvfa.2009.10.006.

OIE. (2012). Caprine and ovine brucellosis (excluding

Brucella ovis). OIE Terrestrial Manual 2012,

(May), 968–977.

Oliveira, S. C., de Almeida, L. A., Carvalho, N.

B., Oliveira, F. S., and Lacerda, T. L. S.

(2012). Update on the role of innate immune

receptors during Brucella abortus infection.

Veterinary Immunology and Immunopathology,

(1–2), 129–135. https://doi.org/10.1016/j.

vetimm.2011.05.036.

Rahman, A. K. M. A., Saegerman, C., Berkvens, D.,

Fretin, D., Gani, O., Uddin, M., & Emmanuel, A.

(2013). Bayesian estimation of true prevalence,

sensitivity and specificity of indirect ELISA,

Rose Bengal Test and Slow Agglutination Test

for the diagnosis of brucellosis in sheep and

goats in Bangladesh. Preventive Veterinary Medicine, 110(2), 242–252. https://doi.

org/10.1016/j.prevetmed.2012.11.029.

Rajashekara, G., Eskra, L., Mathison, A., Petersen,

E., Yu, Q., Harms, J., and Splitter, G.

(2006). Brucella: functional genomics and

host–pathogen interactions. Animal Health

Research Reviews, 7(1–2), 1–11. https://doi.

org/10.1017/S146625230700117X.

Starr, T., Ng, T. W., Wehrly, T. D., Knodler, L. A.,

and Celli, J. (2008). Brucella intracellular

replication requires trafficking through the late

endosomal/lysosomal compartment. Traffic,

(5), 678–694. https://doi.org/10.1111/j.1600-

2008.00718.x.

Sudibyo, A. (1994). Studi Brucellosis dan

Karakterisasi Protein Antigenik Brucella

abortus Isolat Lapang pada Sapi Perah. Bogor:

Program Pasca Sarjana, Institut Pertanian

Bogor.

Walker, R. . (1990). Veterinary Microbiology. (Hirsh

DC and Zee YC, Ed.). Masacuset: Blackwell

science.



DOI: https://doi.org/10.22146/jsv.27546

Article Metrics

Abstract views : 6198 | views : 8936

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats