* Corresponding Author
Deteksi Spesies Brucella pada Kambing di Rumah Potong Hewan Jakarta
https://doi.org/10.22146/jsv.27546 Mujiatun Mujiatun(1*), Retno Damajanti Soejoedono(2), Etih Sudarnika(3), Susan Maphilindawati Noor(4)
(1) Balai Besar Uji Standar Karantina Pertanian, Bandan Karantina Pertanian
(2) Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(3) Balai Besar Penelitian Veteriner -Bogor, Badan Penelitian dan Pengembangan Pertanian Bogor
(4) 3Balai Besar Penelitian Veteriner -Bogor, Badan Penelitian dan Pengembangan Pertanian, Bogor
(*) Corresponding Author
Abstract
Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected from
slaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and Polymerase
Chain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limph
and limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.
The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown.
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Alton, G. (1990). Brucella melitensis. In K. Nielsen
and J. Duncan (Eds.), Animal Brucellosis (pp.
–409). Boca Raton, Florida: CRC Press.
Alton, G.G., Jones, L.M., Angus, R.D. V.2. (1988).
Techniques for the Brucellosis Laboratory.
Mujiatun et al.
Paris: Institut National de la Recherche
Agronomique. INRA Press.
Aworh, M. K., Okolocha, E., Kwaga, J., Fasina, F.,
Lazarus, D., Suleman, I., Subuga, P. (2013).
Human brucellosis: seroprevalence and
associated exposure factors among abattoir
workers in Abuja, Nigeria - 2011, 8688, 1–9.
Batson, M. (2006). Step PCR Assay for identification
of classical Brucella strains (pp. 1–9). CSIRO
(AUS): Australian Animal Health (AAHL).
Blasco, J. M. (1992). Diagnosis of Brucella
melitensis infection in small ruminants. In M.
Plommet (Ed.), Prevention of Brucellosis in
the Mediterranean Countries (pp. 272–277).
JOUR, Wegeningen: Pudoc Scientific.
Blasco, J. M., Garin-Bastuji, B., Marin, C. M.,
Gerbier, G., Fanlo, J., Jiménez de Bagués, M. P.,
and Cau, C. (1994). Efficacy of different Rose
Bengal and complement fixation antigens for
the diagnosis of Brucella melitensis infection
in sheep and goats. The Veterinary Record.
https://doi.org/10.1136/vr.134.16.415.
Celli, J., Chastellier, C. De, Franchini, D., Pizarrocerda,
J., Moreno, E., and Gorvel, J. (2003).
Brucella Evades Macrophage Killing via
VirB-dependent Sustained Interactions with
the Endoplasmic Reticulum, 198(4). https://
doi.org/10.1084/jem.20030088.
EC [European Commission]. (2001). Brucellosis
in Sheep and Goats. Scientific Committe on
Animal Health and Animal Welfare, 1–20.
Farina, R. (1985). Current serological methods in B.
melitensis diagnosis. In : Brucella melitensis.
(Plommet, M., Verger, J. M., eds), Martinus
Nijhoff Publ., Dordrecht, 139-146.
Ficht, T. A. (2003). Intracellular survival of Brucella:
Defining the link with persistence. Veterinary
Microbiology, 92(3),213–223. https://doi.org/
1016/S0378-1135(02)00367-X.
Gupta, V. K., Nayakwadi, S., Kumar, A., Gururaj, K.,
Kumar, A., and Pawaiya, R. S. (2014). Markers
for the molecular diagnosis of brucellosis in
animals. Adv. Anim. Vet. Sci, 2, 31–39.
Harlow E and Lane, D. (1988). Antibodies A
Laboratory Manual. Cold Spring Harbor
Laboratory. New York.
John, W., Cherwonogrodzky, Dubray, G., Moreno, E.,
M. H. (1990). Antigens of Brucella. In: Animal
Brucellosis. (J. R. Nielsen, K., Duncan, Ed.).
Boca Raton: and 5 and Press Inc.
MacMillan, A. 1990. Conventional Serological Tests.
In: Animal Brucellosis. (Nielsen, K., Duncan,
J.R., eds). CRC Press Inc., Boca Raton, pp.
-198.
Nabukenya, I., Kaddu-mulindwa, D., and Nasinyama,
G. W. (2013). Survey of Brucella infection and
malaria among Abattoir workers in Kampala
and Mbarara Districts , Uganda. BMC Public
Health, 13(1), 1. https://doi.org/10.1186/1471-
-13-901.
Nasinyama, G., Ssekawojwa, E., Opuda, J., Grimaud,
P., Etter, E., and Bellinguez, A. (2014).
Brucella sero-prevalence and modifiable risk
factors among predisposed cattle keepers and
consumers of un-pasteurized milk in Mbarara
and Kampala districts, Uganda Abstract :,
(4), 2–5.
OIE. (2009). Bovine Brucellosis. OIE Terrestrial
Manual 2009, (May), 1–35. https://doi.
org/10.1016/j.cvfa.2009.10.006.
OIE. (2012). Caprine and ovine brucellosis (excluding
Brucella ovis). OIE Terrestrial Manual 2012,
(May), 968–977.
Oliveira, S. C., de Almeida, L. A., Carvalho, N.
B., Oliveira, F. S., and Lacerda, T. L. S.
(2012). Update on the role of innate immune
receptors during Brucella abortus infection.
Veterinary Immunology and Immunopathology,
(1–2), 129–135. https://doi.org/10.1016/j.
vetimm.2011.05.036.
Rahman, A. K. M. A., Saegerman, C., Berkvens, D.,
Fretin, D., Gani, O., Uddin, M., & Emmanuel, A.
(2013). Bayesian estimation of true prevalence,
sensitivity and specificity of indirect ELISA,
Rose Bengal Test and Slow Agglutination Test
for the diagnosis of brucellosis in sheep and
goats in Bangladesh. Preventive Veterinary Medicine, 110(2), 242–252. https://doi.
org/10.1016/j.prevetmed.2012.11.029.
Rajashekara, G., Eskra, L., Mathison, A., Petersen,
E., Yu, Q., Harms, J., and Splitter, G.
(2006). Brucella: functional genomics and
host–pathogen interactions. Animal Health
Research Reviews, 7(1–2), 1–11. https://doi.
org/10.1017/S146625230700117X.
Starr, T., Ng, T. W., Wehrly, T. D., Knodler, L. A.,
and Celli, J. (2008). Brucella intracellular
replication requires trafficking through the late
endosomal/lysosomal compartment. Traffic,
(5), 678–694. https://doi.org/10.1111/j.1600-
2008.00718.x.
Sudibyo, A. (1994). Studi Brucellosis dan
Karakterisasi Protein Antigenik Brucella
abortus Isolat Lapang pada Sapi Perah. Bogor:
Program Pasca Sarjana, Institut Pertanian
Bogor.
Walker, R. . (1990). Veterinary Microbiology. (Hirsh
DC and Zee YC, Ed.). Masacuset: Blackwell
science.
DOI: https://doi.org/10.22146/jsv.27546
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