Potency of Medicinal Plants for Eradication of Avian Influenza : In Vitro Test on Vero Cells

https://doi.org/10.22146/jsv.2626

Agus Setiyono(1*), Nurliani Bermawie(2)

(1) Bagian Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor,
(2) Balai Penelitian Tanaman Obat dan Aromatik, Bogor
(*) Corresponding Author

Abstract


Some of medicinal plants indicate their potency as anti-viral such as Sambiloto (Andrographis paniculata Nees), Temu Ireng (Curcuma aeruginosa L.), Beluntas (Pluchea indica L.) Sirih Merah (Piper crocatum) and Adas (Foeniculum vulgare).  Avian Influenza (AI) H5N1 strain viruses used in this study was isolated from field in Cikole area, West Java in July 20th 2007.  To explore the potency of medicinal plants as anti-viral substance, the consecutive assays were performed by virus infection inhibition test in in vitro study using Vero cells.  After the Vero cells were growing confluently, they were treated with sterilized-extract of medicinal plants either in single or combination.  Furthermore, the culture cells were infected with AI H5N1 strain virus, then incubated at 37oC and examined for cytopathic effect (CPE) microscopically.  The result showed that extract of Sambiloto and combination of Sambiloto and Temu Ireng were stronger than others in inhibition of virus attachment and infection to the cells.  The Vero cells still alive up to 3rd day post infection with AI H5N1 virus after treatment with Sambiloto and Temu Ireng.  In conclusion, extract of Sambiloto and Temu Ireng showed their potency as candidate for anti-viral substances that may needed for eradicating AI infection.



Keywords


Avian Influenza, Medicinal Plants, H5N1 Virus

Full Text:

PDF



DOI: https://doi.org/10.22146/jsv.2626

Article Metrics

Abstract views : 1490 | views : 5211

Refbacks

  • There are currently no refbacks.




Copyright (c) 1970 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats