Pengembangan Media Transpor untuk Koleksi Sampel Preputium, untuk deteksi Bovine Genital Campylobacteriosis

Apris Beniawan; Agustin Indrawati; Fachriyan Hasmi Pasaribu

doi:10.22146/jsv.25912

Pengembangan Media Transpor untuk Koleksi Sampel Preputium, untuk deteksi Bovine Genital Campylobacteriosis

https://doi.org/10.22146/jsv.25912

Apris Beniawan^(1*), Agustin Indrawati⁽²⁾, Fachriyan Hasmi Pasaribu⁽³⁾

(1) Badan Karantina Pertanian, Kementerian Pertanian
(2) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(3) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(*) Corresponding Author

Abstract

Campylobacter fetus subsp. Venerealis (Cfv) is bacteria causing contagious genital diseases in cows called Bovine Genital Campylobacteriosis (BGC) or vibriosis. Isolation of Cfv is difficult, because the bacteria are fragile and need specific nutrients and oxygen (5-10%). The transport media is very important to maintain Cfv survival before culturing in laboratory. The aim of this study was to modify a new transport media as an alternative media for Cfv. Developed media capability was compared to Weybridge media, and Phosphate Buffered Saline (PBS). All transport media was contaminated by Cfv with concentrations of 10⁵,10⁴,10³,10²,10¹(CFU/ml), and was stored for <6, 24, 48, 72, and 96 hours in each transport medium before culturing on blood agar, all in triplicate. The quality of transport media was analyzed based on bacterial growth on blood agar. PCR test was used as a confirmatory test of growing bacteria cultured on blood agar. Based on culture results, Cfv stored in three transport mediums for <6 hours, Cfv grew on blood agar from all concentration levels provided. Cfv stored for 24, 48, 72 and 96 hours on PBS did not grow, whereas on developed media and Weybridge media, the bacteria could grow and enrichment occurs at all concentration levels given. This study indicated the developed media can be used as an alternative transport medium for Cfv bacteria.

Keywords

Bacteria Culture; Campylobacter fetus subs. venerealis (Cfv); developed media; Transport Media

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References

Clark, B.L. (1971). Review of Bovine Vibriosis. Australian Vet J. 47:103-107.

Eaglesome, M.D. and Garcia, M.M. (1992). Microbial agents associated with bovine genital tract infections and semen. Part I. Brucella abortus, Leptospira, Campylobacter fetus and Tritrichomonas foetus. Vet Bull. 62(8):743-775.

George, H.A., Hoffman, P.S., Smibert, R.M. and Krieg, N.R. (1978). Improved media for growth and aerotolerance of Campylobacter fetus. J Clin Microbiol 8(1): 36-41.

Hardjoutomo, S. (1998). Tinjauan tentang Vibriosis Sapi di Indonesia. Balai Penelitian Veteriner. Bogor.

Hum, S. (2007). Vibriosis of Cattle. New South Wales Department of Primary Industries. Australia. Primefact 451.

Hum, S., Hornitzky, M., Berg, T. (2009). Bovine Genital Campylobacteriosis. Australia and New Zealand Standard Diagnostic Procedures, SCAHLS, ed. (Department of Agriculture, Fisheries and Forestry).

[Kementan RI] Kementerian Pertanian Republik Indonesia. (2009). Keputusan Menteri Pertanian Nomor 3238/Kpts/Pd.630/9/2009: Tentang Penggolongan Jenis-Jenis Hama Penyakit Hewan Karantina, Penggolongan dan Klasifikasi Media Pembawa. Jakarta (ID): Kementerian Pertanian Republik Indonesia.

Koya, A. (2016). Bovine Genital Campylobacteriosis: Isolation, identification and virulence profiling of Campylobacter fetus subsp. venerealis in a small animal model. Thesis for the degree of Doctor of Philosophy. School of Veterinary Science. University of Queensland.

Lander, K.P. (1990). The application of a transport and enrichment medium to the diagnosis of Campylobacter fetus infections in bulls. British Vet J. 146:334-340.

Monke, H.J., Love, B.C., Wittum, T.E., Monke, D.R. and Byrum, B.A. (2002). Effect of transport enrichment medium, transport time, and growth medium on the detection of Campylobacter fetus subsp. venerealis. J Vet Diagn Investigation. 14:35-39.

Muller, W., Hotzel, H. and Schulze, F. (2003). Identification and differentiation of Campylobacter fetus subspecies by PCR. Dtsch Tierarztl Wochenschr 110(2): 55-59.

Ng, L., Sherburne, R., Taylor, D.E. and Stiles, M.E. (1985). Morphological forms and viability of Campylobacter species studied by electron microscopy. J Bacteriol. 164(1):338-343.

[OIE] World Organization for Animal Health. (2012). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, World Organisation for Animal Health.

Skirrow, M.B. (1994). Diseases due to Campylobacter, Helicobacter and related bacteria. J Comparative Pathol. III:113-149.

Srijan, A., Bodhidatta, L., Mason,CJ., Bunyarakyothin, G., Jiarakul, W. and Vithayasai, N. (2013). Field Evaluation of a Transport Medium and Enrichment Broth for Isolation of Campylobacter Species from Human Diarrheal Stool Samples. Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences (AFRIMS), Open Journal of Medical Microbiology (3, 2013): 48-52.

Truyers, I., Luke, T., Wilson, D. and Sargison, N. (2014). Diagnosis and management of venereal campylobacteriosis in beef cattle. BMC Veterinary Research 2014, 10:280.

van Vliet, A.H.M., Ketley, J.M., Park, S.F. and Penn, C.W. (2002). The role of iron in Campylobacter gene regulation, metabolism and oxidative stress defence. FEMS Microbiology Reviews 26 (2002): 173-186.

DOI: https://doi.org/10.22146/jsv.25912