* Corresponding Author
Deteksi Molekuler Virus Infectious Bursal Disease (IBD) pada Samp l Bursa Fabrisius yang Diperoleh dari Ayam Terdiagnosa Penyakit IBD
https://doi.org/10.22146/jsv.17890 Michael Haryadi Wibowo(1*), Radhian Fadiar(2), Dito Anggoro(3), Sidna Artanto(4), Surya Amanu(5), Agnesia Endang Tri Hastuti Wahyuni(6)
(1) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(2) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(3) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(4) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(5) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(6) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(*) Corresponding Author
Abstract
Fabrisius sebanyak 5 yang diperoleh pada kasus terdiagnosa IBD, dikoleksi dari peternakan ayam komersial di Yogyakarta. Konfirmasi diagnosis dilakukan dengan metode RT-PCR. Sampel positip uji RT-PCR yang mengamplifikasi fragmen gen VP2. Isolasi virus IBD yang dilakukan kultur in ovo pada telur ayam berembrio (TAB) antibodi negatif terhadap virus IBD, berumur 11 hari. Desposisi materi inokulasi dilakukan pada (CAM), diinkubasi selama lima hari. Panen virus dilakukan dengan mengkoleksi membran korioalantois dan embrio, selanjutnya diamati lesi makroskopis yang timbul akibat infeksi virus IBD. Membran korioalantois dan embrio
selanjutnya digerus dan diproses sebagai suspensi antigen yang digunakan dalam uji AGP. Hasil uji RT-PCR terhadap lima sampel Bursa Fabrisius yang dikoleksi dari peternakan ayam terdiagnosa penyakit IBD, tiga sampel menunjukkan hasil positif teramplifikasi fragmen gen VP-2 virus IBD dengan produk amplifikasi sebesar 440 bp, sedangkan dua sampel sisanya menunjukkan hasil negatif. Uji AGP dengan sumber antigen CAM menunjukkan hasil positip 2 dari 3 sampel yang diuji, sedangkan sumber antigen embrio menunjukkan
hasil negatif. Berdasarkan data yang diperoleh dalam penelitian ini dapat disimpulkan bahwa uji RT-PCR dapat digunakan dalam mendeteksi virus IBD dari sampel BF terdiagnosa IBD. Uji AGP dengan sumber antigen CAM menunjukkan hasil lebih baik dari pada embrionya.
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DOI: https://doi.org/10.22146/jsv.17890
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