* Corresponding Author
Deteksi Molekuler Virus Infectious Bursal Disease (IBD) pada Samp l Bursa Fabrisius yang Diperoleh dari Ayam Terdiagnosa Penyakit IBD
https://doi.org/10.22146/jsv.17890 Michael Haryadi Wibowo(1*), Radhian Fadiar(2), Dito Anggoro(3), Sidna Artanto(4), Surya Amanu(5), Agnesia Endang Tri Hastuti Wahyuni(6)
(1) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(2) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(3) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(4) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(5) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(6) Departemen Mikrobiologi, Fakultas Kedokteran Hewan, UGM
(*) Corresponding Author
Abstract
Fabrisius sebanyak 5 yang diperoleh pada kasus terdiagnosa IBD, dikoleksi dari peternakan ayam komersial di Yogyakarta. Konfirmasi diagnosis dilakukan dengan metode RT-PCR. Sampel positip uji RT-PCR yang mengamplifikasi fragmen gen VP2. Isolasi virus IBD yang dilakukan kultur in ovo pada telur ayam berembrio (TAB) antibodi negatif terhadap virus IBD, berumur 11 hari. Desposisi materi inokulasi dilakukan pada (CAM), diinkubasi selama lima hari. Panen virus dilakukan dengan mengkoleksi membran korioalantois dan embrio, selanjutnya diamati lesi makroskopis yang timbul akibat infeksi virus IBD. Membran korioalantois dan embrio
selanjutnya digerus dan diproses sebagai suspensi antigen yang digunakan dalam uji AGP. Hasil uji RT-PCR terhadap lima sampel Bursa Fabrisius yang dikoleksi dari peternakan ayam terdiagnosa penyakit IBD, tiga sampel menunjukkan hasil positif teramplifikasi fragmen gen VP-2 virus IBD dengan produk amplifikasi sebesar 440 bp, sedangkan dua sampel sisanya menunjukkan hasil negatif. Uji AGP dengan sumber antigen CAM menunjukkan hasil positip 2 dari 3 sampel yang diuji, sedangkan sumber antigen embrio menunjukkan
hasil negatif. Berdasarkan data yang diperoleh dalam penelitian ini dapat disimpulkan bahwa uji RT-PCR dapat digunakan dalam mendeteksi virus IBD dari sampel BF terdiagnosa IBD. Uji AGP dengan sumber antigen CAM menunjukkan hasil lebih baik dari pada embrionya.
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Daftar Pustaka
Allan, W.H., Faragher, J.T. and Cullen, G.A. (1972)
Immunosuppression by the Infectious bursal
agent in chickens immunized against Newcastle
disease. Vet. Rec. 90:511–512.
Anonimus (1979) Methods for examining poultry
biologics for identifying and quantifying avian
pathogen. Subcommitte, national academic
science, Washington D.C. : 109-125.
Anonimus (2001) Manual of Standards Ddiagnostic
Tests and Vaccines Infectious Bursal Disease
(http://education.vetmed.vt.edu/Curriculum/
VM8124/VM8124toth_2002/VirDis/avia/avia.
htm#reoviridae)
Ashraf, S., Tang, y. and Saif, M. (2007)
Development of differential RT-PCR assays and
molecular characterization of the complete VP1
gene of five strains of very virulent infectious
bursal disease virus. Avian Dis. 51 (4): 935-941.
Barlic-Maganja, D., Zorman-Rojs, O. and Grom, J.
(2002) Detection of infectious bursal disease
virus in different lymphoid organ by single-step
reverse transcriptase polymerase chain reaction
and microplate hybridization assay. J. Vet.
Diagn. Invest. 14: 243-246.
Beard, C.W. (1989) Serologic procedures. Dalam:
Purchase, H.G, Arp, H.L., Domermuth, C.H.
and Pearson, J.E. (Eds). A Laboratory manual
for the isolation and identification avian
pathogens. 3rded. Kendal/Hunt publishing
company, Iowa : 192-200.
Becht, H., Muller, H. and Muller, H.I.S. (1998)
Comparative studies of structural and antigenic
properties of two serotype of infectious bursal
disease virus. J. Gen. Virol. 69: 631-640.
Cao, Y.C., Young, W.S. and Law, M. (1998)
Molecular characterization of seven chineese
isolates of infectious bursal diseases virus:
classical, very virulent and variant strain. Avian
Dis. 42: 340-351.
Cavanagh, D. and Naqi, S.A. (2003) Infectious
bronchitis. In: Diseases of poultry, 11th ed. Saif,
Y.M. (Ed). Iowa State University Press, Ames,
Iowa, USA : 101-119.
Hirai, K., Kunihiro, K. and Shimakura, S. (1974)
Characterization of immunosupression in
chickens by infectious bursal disease virus.
Avian Dis. 23:950-965.
Hitchner, S.B. (1970) Infectivity of infectious bursal
disease virus for embryonating eggs. Poult. Sci.
, 511-516.
Kataria, R.S., Tiwari, A.K., Nanthakumar, T. and
Goswani, P.P. (2001) One-step RT-PCR for the
Detection of infectious bursal disease virus in
clinical samples. Vet. Res. Comm. 25(5): 429-
Kaufer, I. and Weiss, E. (1980) Significance of bursa
fabricius as target organ in infectious bursal
disease of chickens. Infect. Immun. 27: 364-367.
Kresno, S.B. (2000) Imunologi: Diagnosis dan
prosedure. Edisi III. Penerbit Fakultas
Kedokteran, Universitas Indonesia, Jakarta :
-273.
Lin, T.L., Wu C.C., Rosenberger, J.K., and Saif, Y.M.
(1994) Rapid differentiation of infectious bursal
diseases virus serotypes by polymerase chain
reaction. J.Vet. Diagn. Invest. 6: 100-102.
Lukert, P.D. and Saif, Y.M. (2003) Infectious bursal
disease. In: Diseases of poultry, 11th ed, Saif,
Y.M. (Ed). Iowa state university press, Ames,
Iowa, USA : 161-179.
Mahgoub, H.A. (2012) An overview of infection
bursal disease. Arch Virol. 157: 2047-2057.
Mawgod, S.A., Arafa, A.S. and Hussein, H.A. (2014)
Molecular genotyping of infectius bursal
disease virus isolated from broiler flock in
agypt. Int. J. Vet. Sci. and Med.. 2: 46-52.
Müller, H., Islam, R.Md. and Raue, R. (2003)
Research on infectious bursal disease: The past,
the present and the future. Vet. Microbiol. 97:
-165.
Müller, R., Kaufer, I., Reinacher, M. and Weiss, E.
(1979) Immunofluorscent studies of early virus
propagation after oral infection with infectious
bursal disease virus (IBDV). Zentralbl. Veterinarmed.
B, 26, 345-352.
Mutinda, U.W., Njagi, L.W., Nyaga, P.N., Debora,
L.C., Mbuthia, P.G., Kemboi, D., Githinji,
J.W.K. and Mariuki, A. (2015) Isoaltion of
infectious bursal disease virus using indigenous
chicken embryos in kenya. Int Scholarly Res.
N o t i c e s , I D 4 6 4 3 7 6 ,
Nagarajan, M.M. and Kibenge, F.S.B. (1997)
Infectious bursal disease virus: A review of
molecular basis for variations in antigenicity
and virulence. Can. J.Vet. Res. 61: 81-88.
Nunoya, T., Otaki, Y., Tajima, M., Hiraga, M., and
Saito, T. (1992) Occurrence of acute infectious
bursal disease with high mortality in japan and
pathogenicity of field isolates in specific
pathogen free chickens. Avian Dis. 36, 597-609.
Putra, H.H., Wibowo, M.H., Untari, T. dan Kurniasih
(2012) Studi lesi makroskopis dan mikroskopis
embrio ayam yang diinfeksi virus newcastle
disease isolat lapang yang virulen. J. Sain Vet.
(1): 57-67.
Raj, D.G., Thangavelu, R., Govindarajan, K.,
Nachimuthu and Venugopalan, A.T. (1995)
Precipatation reaction with newcastle disease
virus. Trop. Anim. Hlth. Prod. 27: 71-75.
Singh J., Banga H.S., Brar R.S., Singh N.D., Sodhi S.
a n d L e i s h a n g t h e m G . D . ( 2 0 1 5 )
Histopathological and immunohistochemical
http://dx.doi.org/10.1155/2015/464376.
diagnosis of infectious bursal disease in poultry
birds. Vet. Word 8(11): 1331-1339.
Sivanandan, V. and Maheswaran, S. K. (1980)
Immune profile of infectious bursal disease. I.
Effect of infectious bursal disease virus on
peripheral blood T and B lymphocytes in
chickens. Avian Dis. 24:715–725.
Tabbu., C.R. (2000) Penyakit ayam dan
penanggulangannya. Vol. 1. Kanisius:
Yogyakarta : 213-221.
Tripathy, N.D. and Reed, W.M. (2003) Pox. In:
Diseases of poultry, 11th ed. Saif, Y.M. (Ed).
Iowa State University Press, Ames, Iowa, USA :
-269.
Van den Berg, T.P. (2000) Acute infectious bursal
disease in poultry: A Rev. Avian Pathol. 29 (3):
-194.
Wibowo, M.H. (2003) Identifikasi serologis virus
infectious laryngotracheitis isolat mangestoni
farm dengan uji agar gel presipitasi dan uji
netralisasi. J. Sain Vet. 21 (2): 1-5.
Wibowo, M.H., Susetya, H. Untari, T., Wahyuni,
A.E.T.H. Tabbu, C.R.dan Asmara, W. (2007)
Identifikasi molekuler virus avian influenza
yang diisolasi dari kasus dengan dan tanpa
gejala klinis khas penyakit avian influneza. J.
Vet. 8 (3): 103-110.
Wibowo, M.H., Untari, T. dan Wahyuni, A.E.T.H.
(2012) Isolasi, identifikasi, sifat fisik dan
biologik virus tetelo yang diisolasi dari kasus di
lapangan. J. Vet.13 (4) 425-433.
DOI: https://doi.org/10.22146/jsv.17890
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