Karakterisasi dan Deteksi Cepat Bakteri Penyebab Penyakit Darah pada Pisang

https://doi.org/10.22146/jpti.9390

Nur Edy(1), Siti Subandiyah(2*), Christanti Sumardiyono(3), Jaka Widada(4)

(1) Fakultas Pertanian Universitas Tadulako Palu
(2) Fakultas Pertanian Universitas Gadjah Mada Yogyakarta
(3) Fakultas Pertanian Universitas Gadjah Mada Yogyakarta
(4) Fakultas Pertanian Universitas Gadjah Mada Yogyakarta
(*) Corresponding Author

Abstract


Blood disease of banana is one of the most serious banana disease in Indonesia. Although the disease has became the subject of quarantine it eventually spread and found in most provinces in Indonesia. The aim of this research were to identify the blood disease bacterium (BDB) using morphological observation, biochemical assay, pathogenicity testing of hosts range using infectivity titration and rapid detection by Polymerase Chain Reaction (PCR). The results showed that the blood disease bacterium could be differentiated from Ralstonia solanacearum race 2, the causal agent of Moko disease and R. solanacearum tobacco isolates. BDB isolates were not able to hydrolyze gelatin, Tween 80, starch, and were not able to produce nitrite from nitrate. They were only able to produce acid from galactose and glycerol. The pathogenicity test indicated that the BDB was only able to infect the banana/plantain and was not able to infect tomato, eggplant, and chili. Rapid detection using PCR method showed that the 121F/R primers was able to amplify the BDB genome and was not able to amplify the genome of R. solanacearum tobacco isolates.


Keywords


Blood Disease Bacterium; characterization; Polymerase Chain Reaction

Full Text:

PDF


References

Ausubel, F.M., R. Brent., R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, & K. Struhl (eds.). 1992. Current Protocols in Molecular Biology. Greene Publishing Association; WileyInterscience, New York.

Baharuddin. 1994. Pathological, Biochemical, and Serological Characterization of the Blood Disease Bacterium Affecting Banana and Plantain. Cuvillier Verlag, Gottingen, Doctoral Dissertation. Eden-Green, S.J. 1994. Diversity of Pseudomonas solanacearum and Related Bacteria in South Asia: New Direction of Moko Disease, p. 25–34. In A.C. Hayward & G.L. Hartman (eds.). Bacterial Disease and its Causative Agent Pseudomonas solanacearum. CAB International, Wellington.

Fahy, P.C. & A.C. Hayward. 1983. Media and Methods for Isolation and Diagnostic Tests, p. 337–378. In P.C. Fahy & G.J. Persley (eds.), Plant Bacterial Diseases: a Diagnostic Guide. Academic Press, Sidney. Fegan, M., M. Taghavi, Sly Li, & A.C. Hayward. 1998. Phylogeny, Diversity, and Molecular Diagnostic of Ralstonia solanacearum, p. 19–33. In P.C. Prior, C.Allen, J. Elphinstone. (eds.), Bacterial Wilt, Molecular, and Ecological Aspects. Berlin Heidelberg, Germany and Paris, Frances. Springer-Verlag and INRA Edition.

Fegan, M. & P. Prior. 2005. How Complex is the "Ralstonia solanacearum species complex"?, p. 449–461. In C. Allen, P. Prior, & A.C. Hayward (eds.), Bacterial Wilt Disease and the Ralstonia solanacearum Species Complex. American Phytopathological Society Press, St. Paul, Minnesota.

Fegan, M. & P. Pror. 2006. Diverse Members of the Ralstonia solanacearum Species Complex Cause Bacterial Wilt of Banana. Australian Plant Pathology 35: 93–101.

French, E.R. & L. Sequiera. 1970. Strains of Pseudomonas solanacearum from Central and South America: Comparative Study. Phytopathology 60: 506-512.
Hayward, A.C. 2000. Ralstonia solanacearum. Encyclopedia of Microbiology 4: 32–42.

Kelman, A. 1953. The Bacterial Wilt Caused by Pseudomonas solanacearum. A Literary Review and Bibliography. Technical Bulletin of North Carolina Agricultural Experiment Station No. 99. Raleigh, North Carolina. 194 p.
Lelliot, R.A. & D.E. Stead. 1987. Methods in Plant Pathology. Vol. 2. Methods for the Diagnosis of Bacterial Diseases of Plants. British Society for Plant Pathology by Blackwell Scientific Publication, Oxford. 216 p.
Opina, N., F. Tavner, G. Holloway, J. Wang, T. Li, R. Maghirang, M. Fegan, A. Hayward, V. Krishnapillai, W. Hong, B. Holloway, & J. Timmis. 1997. A Novel Method for Development of Species and Strain-Specific DNA Probes and PCR Primers for Identifying Burkholderia solanacearum (formerly Pseudomonas solanacearum). Asia-Pacific Journal of Molecular Biology and Biotechnology 5: 19–30.

Roberts, S.J. Eden-Green, P. Jones, & D.J. Ambler. 1990. Pseudomonas syzygii sp. Nov., the Cause of Sumatera Disease of Cloves. Systematic and Applied Microbiology 13: 34–43.

Seal, S.E., L. Jackson, & M.J. Daniels. 1992a. Isolation of a Pseudomonas solanacearum, specific DNA probe by subtraction hybridization and Construction of Species-species Oligonucleotida Primers for Sensitive Detection by Polymerase Chain Reaction. Applied and Environmental Microbiology 59: 3751–3758.

Seal, S.E. & J.G. Elphinstone. 1994. Advances in Identification and Detection of Pseudomonas solanacearum, p. 35–57. In A.C. Haywards & G.L. Hartman (eds.), Bacterial Wilt: the Disease and its Causative Agent, Pseudomonas solanacearum. CAB International.

Wallingford, UK. Sequeira, L. 1997. Bacterial Wilt: The Missing Element in International Banana Improvement Programs, p. 6–14. In P. Prior, C. Allen, & J. Elphinstone (eds.) Bacterial Wilt Disease: Molecular and Ecological Aspect, Reports of the Second International Bacterial Wilt Symposium.
France. Subandiyah, S. 1991. Bacterial Wilt of Peanut Caused by Pseudomonas solanacearum F.F. Smith. Thesis master. The University of Queensland, St. Lucia, Queensland, Australia.

Thagavi, M., A.C. Hayward, L.I. Sly, & M. Fegan. 1996. Analysis of Phylogenetic Relationship of Strains Bulkhoderia solanacearum, Pseudomonas syzygii, Blood Disease Bacterium based on 16S rRNA Gene Sequences. International Journal of Systematic Bacteriology 46: 10–15.



DOI: https://doi.org/10.22146/jpti.9390

Article Metrics

Abstract views : 4661 | views : 3324

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2011 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics