Analisis Variabilitas Genetik Rigidoporus microporus (Jamur Akar Putih) pada Karet dengan Teknik PCR-RFLP Fragment ITS

https://doi.org/10.22146/jpti.9383

Ria Novianti(1), Haryono Semangun(2), Ferry F. Karwur(3*), Martanto Martosupono(4)

(1) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(2) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(3) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(4) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(*) Corresponding Author

Abstract


The eleven isolates studied fell into two groups, which showed that the fungus did not have high genetic variability. Isolates from diseased rubber trees in shorter vicinities, which were due to pathogen’s vegetative spread through root contact, have close genetic relationship. Meanwhile isolates from far apart trees, either in one estate or from different islands, does not necessarily have distant genetic relationship. It might be due to the fact that long distance dispersal of the disease could also due to rhizomorph contaminating rubber stumps. PCR-RFLP Fragment ITS Techniques can be applied to study genetic relationship of Rigidoporus microporus isolates. It is expected that the technique will be applied with more isolates from different provinces by using additional different restriction enzymes.


Keywords


genetic variability; PCR-RFLP Fragment ITS Technique; Rigidoporus microporus; white root rot of rubber

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References

Campbell, N.A., J.B. Reece, & L.G. Mitchell. 2003. Biologi edisi kelima. Jilid II. (diterjemahkan dari: Biology, 5 ed., penerjemah: W. Manalu). Erlangga, Jakarta. 404 p.

Farid, A.M., S.S. Lee, H. Mohd. Rosli, & M. Patahayah. 2006. Specific Detection of the White Root Disease Fungus, Rigidoporus lignosus, through a PCR-based Technique. Univ. Sains Malaysia, Penang.

Hamidson, S.H. & S. Naito. 2004. Distribution of Rigidoporus lignosus Genotypes in a Rubber Plantation, as Revealed by Somatic Compatibility. Mycoscience 45: 72–75.

Jasalavich, C.A., A. Ostrofsky, & J. Jellison. 2000. Detection and Identification of Decay Fungi in Spruce Wood by Restriction Fragment Length Polymorphism Analysis of Amplified Genes Encoding rRNA. Applied and Environmental Microbiology 66: 4725–4734.

Semangun, H. 2007. White Root Disease of Rubber: Some of its Biological Aspects, p. 60–67. In S. Prawirosoemardjo, B. Setyawan, H. Suryaningtyas, M. Supriadi (eds.), Proceeding of International Workshop on White Root Disease of Hevea Rubber, Salatiga, 28–29 November 2006.

Semangun, H. 2008. Penyakit-Penyakit Tanaman Perkebunan di Indonesia. ed. kedua, Gadjah Mada University Press, Yogyakarta. 835 p.

Suwandi. 2007. Mode of Dispersal and Variation in Population of White Root Fungus Rigidoporus microporus as Revealed by Mycelial Incompatibility, p. 68–75. In S. Prawirosoemardjo, B. Setyawan, H. Suryaningtyas, M. Supriadi (eds.), Proceeding of International Workshop on White Root Disease of Hevea Rubber, Salatiga, 28–29 November 2006.



DOI: https://doi.org/10.22146/jpti.9383

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