Ekstraksi DNA Phytophthora palmivora Isolat Kelapa
Achmadi Priyatmojo(1*), Siti Subandiyah(2)
(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Coconut isolate of Phytophthora palmivora DNA was extracted from sporangium and mycelium using SDS and Proteinase-K method. Sporangium and mycelium were harvested from liquid and solid culture of Potato Dextrose medium. The result of the study showed that the DNA extracted from sporangium and mycelium of 8 days solid culture was seen as distinct band. On the other hand, the dNA from sporangium and mycelium of 24 days old liquid culture was fragmented with smear band appearance.
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DOI: https://doi.org/10.22146/jpti.9358
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