Propagation and Purification of Baculovirus oryctes Huger

https://doi.org/10.22146/jpti.9317

Susamto Somowiyarjo(1*), YB Sumardiyono(2), Sedyo Hartono(3), Triharso Triharso(4), Jun Kobayashi(5)

(1) Faculty of Agriculture Universitas Gadjah Mada
(2) Faculty of Agriculture Universitas Gadjah Mada
(3) Faculty of Agriculture Universitas Gadjah Mada
(4) Faculty of Agriculture Universitas Gadjah Mada
(5) Mie University
(*) Corresponding Author

Abstract


An isolate of Baculovirus oryctes, a possible biological control agent for coconut beetle (Oryctes rhinoceros Huger) from East Java was propagated and purified. The virus could be transmitted by feeding the imago with 10% sucrose containing virus from homogenate of infected beetles. Effectivity of virus to 9 healthy females by sexual copulation. Virus be succesfully purified by a method of Payne.


Keywords


Baculovirus oryctes; transmission; purification

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References

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DOI: https://doi.org/10.22146/jpti.9317

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Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

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