* Corresponding Author
Isolation, Characterization, and Selection of Bacillus sp. from Shallot Rhizosphere that Inhibits Fusarium oxysporum Growth
https://doi.org/10.22146/jpti.89634 Alil Saputra(1), Nur Prihatiningsih(2*), Heru Adi Djatmiko(3), Dhadhang Wahyu Kurniawan(4)
(1) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 61, Purwokerto, Central Java 53123 Indonesia
(2) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 61, Purwokerto, Central Java 53123 Indonesia
(3) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 61, Purwokerto, Central Java 53123 Indonesia
(4) Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman Jln. DR. Soeparno No. 63 Purwokerto, Central Java 53122 Indonesia
(*) Corresponding Author
Abstract
Bacillus sp. is a Plant Growth Promoting Rhizobacteria (PGPR) species that lives in the rhizosphere. This bacterium can produce antifungal compounds that suppress pathogenic fungi, such as Fusarium oxysporum. The aim of this research was isolate, characterize, and obtain Bacillus sp. that can inhibit F. oxysporum which causes twisted disease in shallots. Isolation was done by sampling 10 g of soil from shallot roots, placing it in 9 mL of sterile water, and carrying out a series of dilutions. The first dilution was incubated at 80 °C for 20 minutes to obtain Bacillus sp. on the selective medium HiChrome. Colony morphology, shape, cell color, Gram staining, catalase and endospore staining were observed from obtained isolates. An antagonist test was done to determine the inhibitory effects of isolates against the pathogenic fungus F. oxysporum. Four isolates of Bacillus sp. were obtained with irregular colony shape, dull white color without muccus, edges varying in shape between lobate and undulate, and raised elevation. The results of the antagonist test showed that the four isolates had the ability to inhibit the growth of the pathogenic fungus F. oxysporum with the greatest inhibition shown by isolate BM1 at 30.12%, with an antibiosis mechanism and hyphae swelling.
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DOI: https://doi.org/10.22146/jpti.89634
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