* Corresponding Author
Sugarcane streak mosaic virus (ScSMV) Resistance Evaluation of Sugarcane Varieties
https://doi.org/10.22146/jpti.71158 Feny Ernawati(1*), Syamsuddin Djauhari(2), Mintarto Martosudiro(3), Lilik Koesmihartono Putra(4), Ari Kristini(5)
(1) Postgraduate Program, Faculty of Agriculture, Brawijaya University, Jln. Veteran, Malang, East Java, 65145 Indonesia
(2) Faculty of Agriculture, Brawijaya University, Jln. Veteran, Malang, East Java, 65145, Indonesia
(3) Faculty of Agriculture, Brawijaya University, Jln. Veteran, Malang, East Java, 65145, Indonesia
(4) Indonesian Sugar Research Institute, Jln. Pahlawan 25 Pasuruan, East Java, 67126 Indonesia
(5) Indonesian Sugar Research Institute, Jln. Pahlawan 25 Pasuruan, East Java, 67126 Indonesia
(*) Corresponding Author
Abstract
Sugarcane streak mosaic virus (ScSMV) is the most important viral disease of sugarcane in Indonesia with distribution in almost all commercial sugarcane plantations. The disease causes significant yield losses of both cane tonnage and sugar yield. The use of resistant varieties is the best approach for controlling viral diseases. This study aims to investigate resistance response of several introduced varieties against ScSMV in a glasshouse condition and the impact of the viral infection on chlorophyll and proline content in sugarcane leaves. Sugarcane plants were inoculated using ScSMV inoculum one month after planting using an abrasive pad rubbing method. Disease incidence and severity was observed at week 4-12 after inoculation and variety resistance levels were classified based on disease incidence. Confirmation of the virus was done by RT-PCR. Spectrophotometer was used to measure chlorophyll content at dual wavelengths of 645 and 663 nm, and proline content at wavelengths of 520 nm. The results showed that most of the tested varieties were susceptible to ScSMV. There are six highly resistant varieties, namely SRA 1, SRA 2, N 10-4, N 10-7, N10-9, and N 10-13, but these varieties still require to be tested on a field scale. ScSMV infection generally decrease chlorophyll and proline content. However, the physiological effect of ScSMV infection on chlorophyll and proline content needs further investigation.
Keywords
Full Text:
PDFReferences
Agrios. G. N. (2005). Plant Pathology (5th ed.). San Diego, CA, United States: Elsevier Academic Press.
Aldila, F., Anggarwulan, E., & Solichatun. (2008). Kadar Prolin dan Polifenol Tanaman Kimpul (Xanthosoma sagittifolium (L.) Schott) pada Variasi Intensitas Cahaya dan Ketersediaan Air [Research report]. Surakarta, Indonesia: Universitas Sebelas Maret.
Arnon, D. I. (1949). Copper Enzymes In Isolated Chloroplasts. Polyphenol Oxidase in Beta vulgaris. Plant Physiology, 24(1), 1‒15. https://doi.org/10.1104/pp.24.1.1
Bassiouny, F. M., Khalil, R. R., El-Dougdo, K. A., Abo-Elmaty, S., & Yousef, M.S. (2015). Gemini Modified Antioxidant Systems and Prolin Accumulation in Tomato Plant. Journal of Functional and Environmental Botany, 5(1), 31-40.
Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid Determination of Free Proline for Water Stress Study. Plant Soil, 41, 1222-1230.
Bedoya, G. C., Espejel, F. Briseno, R. A., Vela, J. H. & Rosales, L. S. (2011). Short Distance Movement of Genomic Negative Strands in a Host and Nonhost for Sugarcane mosaic virus (SCMV). Virology Journal, 8(15), 1-8.
Chatterjee, A., & Ghosh, S. K. (2008). Alterations in Biochemical Components in Mesta Plants Infected with Yellow Vein Mosaic Disease. Brazilian Society of Plant Physiology, 20(4), 267-275.
Chen, C., & Dickman, M. B. (2005). Proline Suppresses Apoptosis in the Fungal Pathogen Colletotrichum trifolii. Proceedings of the National Academy of Sciences, 102(9), 3459–3464. https://doi.org/10.1073/pnas.0407960102
Damayanti, T. A., Putra, L. K., & Giyanto. (2010). Hot Water Treatment of Cutting-Cane Infected with Sugarcane Streak Mosaic Virus (SCSMV). Journal of ISSAAS (International Society for Southeast Asian Agricultural Sciences, 16(2), 17‒25.
Damayanti, T. A., & Putra, L. K. (2011). First Occurrence of Sugarcane streak mosaic virus Infecting Sugarcane in Indonesia. Journal of General Plant Pathology, 77, 72-74.
Damayanti, T. A., Putra, L. K., & Giyanto. (2011). Kajian Penggunaan Kombinasi Tiga Jenis Bakteri Biokontrol untuk Menekan Infeksi Sugarcane streak mosaic virus (SCSMV) pada Tebu. Majalah Penelitian Gula, 47(1), 40‒53.
Hamida, R., & Suhara, C. (2019). Pengaruh Sugarcane streak mosaic virus terhadap Anatomi dan Kadar Klorofil Daun Beberapa Aksesi Tebu. Berita Biologi, 18(1), 37-45.
Hema, M., Savithri, H. S., & Sreenivasulu, P. (2003). Comparison of Direct Binding Polymerase Chain Reaction with Recombinant Coat Protein Antibody Based Dot-blot Immunobinding assay and Immunocapture-Reverse Transcription-Polymerase Chain Reaction for Detection of Sugarcane streak mosaic virus Causing disease of Sugarcane in India. Current Science, 85(12), 1774-1777.
Hosseini, S. A., Ghaemi, M., & Khayyat, M. (2021). Responses of Pepper to Alfalfa mosaic virus and Manganese Nutrition Under Greenhouse Conditions: Preliminary Results. Journal of Horticulture and Postharvest Research, 4, (Special Issue), 67-80.
Kristini, A., Irawan, & Sasongko, D. (2006). Mosaic Booming. Gula Indonesia, 30(1), 36-38.
Legowo, L. (1993). Rangkuman Hasil Uji Ketahanan Jenis terhadap Penyakit Mosaik, Blendok, Pokkah Boeng selama 28 Tahun (1964–1992). Pasuruan, Indonesia: Pusat Penelitian Perkebunan Gula Indonesia.
Mandadi, K. K., & Scholthof, K. B. G. (2013). Plant Immune Responses against Viruses: How Does a Virus Cause Disease? The Plant Cell, 25(5), 1489-1505.
Magarey, R. C., Kristini, A., Achadian, E., Thompson, N., Wilson E., Reynolds M., Sallam, N., Goebel, R., & Putra, L.K. (2018). Sugarcane Streak Mosaic—Researching a Relatively New Disease in Indonesia. In Proceeding of the 2018 Conference of the Australian Society Sugar Cane Technologists (ASSCT) (pp. 257–266). Annual ASSCT Conference, Mackay, Australia, April 17–20, 2018.
Matthews, R.E.F. (1991). Plant Virology. 3th Edition. San Diego, California: Academy Press.
Mohanty, S. K., & Sridhar, R. (1982). Physiology of Rice tungro virus Diseases: Proline Accumulation due to Infection. Physiology of Plant 56, 89-93.
Pazarlar, S., Gümüş, M., & Öztekin, G. B. (2013). The Effect of Tobacco mosaic virus Infection on Growth and Physiological Parameter in Some Pepper Varieties (Capsicum annuum L). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(2), 427‒433. https://doi.org/10.15835/nbha4129008
Putra, L. K., Ogle, H. J., James, A. P., & Whittle, P. J. L. (2003). Distribution of Sugarcane mosaic virus in Sugarcane Plants. Australasin Plant Pathology, 32, 305-307.
Putra, L. K., & Damayanti, T. A. (2012). Major Disease Affecting Sugar Production in Indonesia. Functional Plant Science and Biotechnology 6(2), 124-129.
Putra, L. K., Kristini, A., Achadian, E. M., & Damayanti, T. A. (2014). Sugarcane Streak Mosaic Virus in Indonesia: Distribution, Characterisation, Yield Losses and Management Approaches. Sugar Tech 16(4), 392-399.
Putra, L. K. (2015). Penyebaran dan Cara Bertahan Sugarcane Streak Mosaic Virus (SCSMV) pada Tebu (Saccharum officinarum L.) di Indonesia [Doctorate thesis]. Malang, Indonesia: Universitas Brawijaya.
Putra, L. K., Hadiastono, T., Syamsidi, S.R.Ch., & Djauhari, S. (2015). Dispersal, Yield Losses and Varietal Resistance of Sugarcane Streak Mosaic Virus (SCSMV) in Indonesia. International Journal of Virology, 11(1), 32-40.
Putra, L.K., Kristini, A., & Jati, W.W. (2022). Viral Disease of Sugarcane in Indonesia: Occurrence Notes, Pathogenic Characteristics and Management Strategies. In P. Zang & T. A. Siswoyo (Eds.), Proceeding of the 5th International Conference on Agriculture and Life Science. AIP Publishing Conference Proceedings. (In press).
Sholeh, A., Sugiharto, B., & Addy, H.S. (2019). Monitoring Sugarcane streak mosaic virus on Recent Sugarcane Varieties in East Java, Indonesia. Journal Applied Science, 19(7), 647-653.
Soni, S. K., Mishra, M. K., Mishra, M., Kumari, S., Saxena, S., Shukla, V., Tiwari, S., & Shirke, P. (2022). Papaya Leaf Curl Virus (PaLCuV) Infection on Papaya (Carica papaya L.) Plants Alters Anatomical and Physiological Properties and Reduces Bioactive Components. Plants2022, 11(579). https://doi.org/10.3390/plants11050579
Srisink, S., Taylor, P. W. J., Stringer, J. K., & Teakle, D. S. (1994). An Abrasive Pad Rubbing Method for Inoculating Sugarcane with Sugarcane mosaic virus. Australian Journal of Agricultural Research, 45(3), 625‒631. https://doi.org/10.1071/AR9940625
Sumithra, K., & Reddy, A. R. (2004). Changes in Proline Metabolism of Cowpea Seedlings Under Water Deficit. Journal of Plant Biology, 31, 201-204.
Valkonen, J. P. T. (2002). Natural Resistance to Viruses. In Khan, J.A. & J. Dijkstra (Eds.), Plant Viruses as Molecular Pathogen. Food Product Press, New York.
Zhao, J., Zhang, X., Hog, Y. & Liu, Y. (2016). Chloroplast in Plant-Virus Interaction. Frontiers in Microbiology, 7, 1565. https://doi.org/10.3389/fmicb.2016.01565
DOI: https://doi.org/10.22146/jpti.71158
Article Metrics
Abstract views : 1570
| 
views : 929
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics