* Corresponding Author
In Vitro Evaluation of Trichoderma asperellum Isolate UGM-LHAF against Rhizoctonia solani Causing Sheath Blight Disease of Rice
https://doi.org/10.22146/jpti.65290 Yeyet Nurhayati(1*), Suryanti Suryanti(2), Arif Wibowo(3)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281, Indonesia
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281, Indonesia
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281, Indonesia
(*) Corresponding Author
Abstract
Trichoderma spp. is a fungus widely used to control soil-borne pathogens, such as Rhizoctonia solani which is plant pathogenic fungi in widely host range, especially on rice. This research aimed to evaluate the ability of Trichoderma asperellum isolate UGM-LHAF against R. solani causing sheath blight disease of rice in vitro condition. Trichoderma sp. used in this research was obtained from The Biological Laboratory of Pakem, Yogyakarta, Indonesia, and Rhizoctonia sp. was obtained through isolation of diseased rice obtained from rice fields in Yogyakarta. The two isolates were characterized base on morphology and molecular identification based on ITS rDNA. The pathogenicity test of Rhizoctonia sp. was evaluated by adding four sclerotia of Rhizoctonia sp. near rice roots at 6 days after sowing. The in vitro test used dual culture and antifungal activity (0%, 10%, 25%, 50% culture filtrate of Trichoderma sp.) with three replicates of each treatment. Two isolates were identified as T. asperellum and R. solani. Sheath blight symptoms appeared after 12 days inoculation. In the in vitro test, T. asperellum isolate UGM-LHAF was able to inhibit the mycelial growth of R. solani (64.23% on dual culture and 68.5% on antifungal activity). This study suggests that T. asperellum isolate UGM-LHAF able to inhibit the growth of R. solani and can be a further potential candidate as a biocontrol agent against R. solani causing sheath blight disease of rice.
Keywords
Full Text:
PDFReferences
Asad, S.A., Ali, N., Hameed, A., Khan, S.A., Ahmad, R., Bilal, M., … Tabassum, A. (2014). Biocontrol Efficacy of Different Isolates of Trichoderma against Soil Borne Pathogen Rhizoctonia solani. Polish Journal of Microbiology, 63(1), 95–103. https://doi.org/10.33073/pjm-2014-014
Badan Pusat Statistik. (2020). Harvested Area, Productivity, and Production of Paddy by Province, BPS Statistics Indonesia. Retrieved from https://www.bps.go.id/indicator/53/1498/1/harvested-area-productivity-and-production-of-paddy-by-province.html
Bissett, J. (1991). A Revision of the Genus Trichoderma. II. Infrageneric Classification. Canadian Journal of Botany, 69(11), 2357–2372. https://doi.org/10.1139/b91-297
Budiarti, S.W., Lukman, R., Wibowo, A., Sumardiyono, C., & Priyatmojo, A. (2020). The Cultural and Morphological Variability among Rhizoctonia solani Isolates Causing Banded Leaf and Sheath Blight of Maize in Indonesia. Archives of Phytopathology and Plant Protection, 53(1–2),17–36. https://doi.org/10.1080/03235408.2020.1715765
Carling, D.E. (1996). Grouping in Rhizoctonia solani by Hyphal Anastomosis Reaction. In B. Sneh, S. Jabaji-Hare, S. Neate, G. Dijst (Eds.), Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control (pp. 35–47), Dordrecht, Netherlands: Springer. https://doi.org/10.1007/978-94-017-2901-7_3
Carling, D.E., Baird, R.E., Gitaitis, R.D., Brainard, K.A., & Kuninaga, S. (2002). Characterization of AG-13, a Newly Reported Anastomosis Group of Rhizoctonia solani. Phytopathology, 92(8), 893–899. https://doi.org/10.1094/PHYTO.2002.92.8.893
Chinnaswami, K., Mishra, D., Miriyala, A., Vellaichamy, P., Kurubar, B., Gompa, … Raman, M.S. (2021). Native Isolates of Trichoderma as Biosuppressants against Sheath Blight and Stem Rot Pathogens of Rice. Egyptian Journal of Biological Pest Control, 31, 12. https://doi.org/10.1186/s41938-020-00356-4
de França, S.K.S., Cardoso, A.F., Lustosa, D.C., Ramos, E.M.L.S., de Filippi, M.C.C., & da Silva, G.B. (2015). Biocontrol of Sheath Blight by Trichoderma asperellum in
Tropical Lowland Rice. Agronomy for Sustainable Development, 35(1), 317–324. https://doi.org/10.1007/s13593-014-0244-3
Dinas Perkebunan Provinsi Kalimatan Timur. (2017). Manfaat Trichoderma sp. dan Cara Perkembangbiakannya. Retrieved from https://disbun.kaltimprov.go.id/artikel/manfaat-trichoderma-sp-cara-pembiakkannya
Doyle, J.J. & Doyle, J.L. (1990). Isolation of Plant DNA from Fresh Tissue. Focus, 12(1), 13–15.
El-Rafai, I.M., Asswah, S.M.W., & Awdalla, O.A. (2003). Biocontrol of Some Tomato Disease Using Some Antagonistic Microorganisms. Pakistan Journal of Biological Sciences, 6(4), 399–406. https://doi.org/10.3923/pjbs.2003.399.406
Garcia, V.G., Onco, M.A.P., & Susan, V.R. (2006). Review. Biology and Systematic of the Form Genus Rhizoctonia. Spanish Journal of Agricultural Research, 4(1), 55–79. https://doi.org/10.5424/sjar/2006041-178
Guleria, S., Aggarwal, R., Thind, T.S., & Sharma, T.R. (2007). Morphological and Pathological Variability in Rice Isolates of Rhizoctonia solani and Molecular Analysis of their Genetic Variability. Journal of Phytopathology, 155(11–12), 654–661. https://doi.org/10.1111/j.1439-0434.2007.01291.x
Jaisani, P. & Pandey, R.N. (2017). Morphological and Molecular Characterization for Identification of Isolates of Trichoderma spp. from Rhizospheric Soils of Crops in Middle Gujarat. Indian Phytopathology, 70(2), 238–245. http://doi.org/10.24838/ip.2017.v70.i2.71652
Jiang, H., Zhang, L., Zhang, L-Z., Ojaghian, M.R., & Hyde, K.D. (2016). Antagonistic Interaction between Trichoderma asperellum and Phytophthora capsici in Vitro. Journal of Zhejiang University Science B, 17(4), 271–281. https://doi.org/10.1631/jzus.B1500243
Khan, A.A. & Sinha, A.P. (2007). Screening of Trichoderma spp. against Rhizoctonia solani the Causal Agent of Rice Sheath Blight. Indian Phytopathology, 60(4), 450–456. Retrieved from https://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/13922/6910
Manganiello, G., Sacco, A., Ercolano, M.R., Vinale, F., Lanzuise, S., Pascale, A., … Woo, S.L. (2018). Modulation of Tomato Response to Rhizoctonia solani by Trichoderma harzianum and Its Secondary Metabolite Harzianic Acid. Frontiers in Microbiology, 9, 1996. https://doi.org/10.3389/fmicb.2018.01966
Mayo-Prieto, S., Campelo, M.P., Lorenzana, A., Rodríguez-González, A., Reinoso, B., Gutiérrez, S., & Casquero, P.A. (2020). Antifungal Activity and Bean Growth Promotion of Trichoderma Strains Isolated from Seed vs Soil. European Journal of Plant Pathology, 158(4), 817–828. https://doi.org/10.1007/s10658-020-02069-8
Meena, M., Swapnil, P., Zehra, A., Dubey, M.K., & Upadhyay, R.S. (2017). Antagonistic Assessment of Trichoderma spp. by Producing Volatile and Non-volatile Compounds against Different Fungal Pathogens. Archives of Phytopathology and Plant Protection, 50(13–14), 629–648. https://doi.org/10.1080/03235408.2017.1357360
Mishra, P.K., Gogoi, R., Singh, P.K., Rai, S.N., Singode, A., Kumar, A., & Manjunatha, C. (2014). Morpho-cultural and Pathogenic Variability in Rhizoctonia solani Isolates from Rice, Maize and Green Gram. Indian Phytopathology, 67(2), 147–154. Retrieved from https://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/40913/18622
Mukhopadhyay, R. & Kumar, D. (2020). Trichoderma: A Beneficial Antifungal Agent and Insights into its Mechanism of Biocontrol Potential. Egyptian Journal of Biological Pest Control, 30(1), 1–8. https://doi.org/10.1186/s41938-020-00333-x
Naeimi, S., Okhovvat, S.M., Javan-Nikkhah, M., Vágvölgyi, C., Khosravi, V., & Kredics, L. (2010). Biological Control of Rhizoctonia solani AG1-1A, the Causal Agent of Rice Sheath Blight with Trichoderma Strains. Phytopathologia Mediterranea, 49(3), 287–300. Retrieved from https://www.jstor.org/stable/26458654
Oszako, T., Voitka, D., Stocki, M., Stocka, N., Nowakowska, J.A., Linkiewicz, A., ... Malewski, T. (2020). Trichoderma asperellum Efficiently Protects Quercus robur Leaves against Erysiphe alphitoides. European Journal of Plant Pathology, 159(2), 295–308. https://doi.org/10.1007/s10658-020-02162-y
Pandian, R.T.P., Raja, M., Kumar, A., & Sharma, P. (2016). Morphological and Molecular Characterization of Trichoderma asperellum strain Ta13. Indian Phytopathology, 69(3), 298–303. Retrieved from https://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/60166/24995
Priyatmojo, A., Escopalao, V.E., Tangonan, N.G., Pascual, C.B., Suga, H., Kageyama, K., & Hyakumachi, M. (2001). Characterization of a New Subgroup of Rhizoctonia solani Anastomosis Group 1 (AG-1-ID), Causal Agent of a Necrotic Leaf Spot on Coffee. Phytopathology®, 91(11), 1054–1061. https://doi.org/10.1094/phyto.2001.91.11.1054
Rabindran, R., & Vidhyasekaran, P. (1996). Development of a Formulation of Pseudomonas fluorescens PfALR2 for Management of Rice Sheath Blight. Crop Protection, 15(8), 715–721. https://doi.org/10.1016/s0261-2194(96)00045-2
Rismanto, T.A.L. (2020). Eksplorasi dan Isolasi APH Trichoderma sp. (Exploration and Isolation of Biological Agents of Trichoderma sp.). Retrieved from https://dpkp.jogjaprov.go.id/
Romero-Cortes, T., López-Pérez, P.A., Pérez España, V.H., Medina-Toledo, A.K., Aparicio-Burgos, J.E., & Cuervo-Parra, J.A. (2019). Confrontation of Trichoderma asperellum VSL80 against Aspergillus niger via The Effect of Enzymatic Production. Chilean Journal of Agricultural & Animal Sciences, 35(1), 68–80. https://doi.org/10.4067/s0719-38902019005000202
Sandoval, R.F.C., & Cumagun, C.J.R. (2019). Phenotypic and Molecular Analyses of Rhizoctonia spp. Associated with Rice and Other Hosts. Microorganisms, 7(3), 88. https://doi.org/10.3390/microorganisms7030088
Saravanakumar, K., Yu, C., Dou, K., Wang, M., Li, Y., & Chen, J. (2016). Synergistic Effect of Trichoderma-derived Antifungal Metabolites and Cell Wall Degrading Enzymes on Enhanced Biocontrol of Fusarium oxysporum f. sp. cucumerinum. Biological Control, 94, 37–46. https://doi.org/10.1016/j.biocontrol.2015.12.001
Shang, J., Liu, B., & Xu, Z. (2020). Efficacy of Trichoderma asperellum TC01 against Anthracnose and Growth Promotion of Camellia sinensis Seedlings. Biological Control, 143, 104205. https://doi.org/10.1016/j.biocontrol.2020.104205
Singh, P., Mazumdar, P., Harikrishna, J.A., & Babu, S. (2019). Sheath Blight of Rice: A Review and Identification of Priorities for Future Research. Planta, 250(5), 1387–1407. https://doi.org/10.1007/s00425-019-03246-8
Singh, R., Murti, S., Mehilal, Tomer, A., & Prasad, D. (2015). Virulence Diversity in Rhizoctonia Solani Causing Sheath Blight in Rice Pathogenicity. Journal of Plant Pathology & Microbiology, 6(8), 296. https://doi.org/10.4172/2157-7471.1000296
Singh, V., Amaradasa, B. S., Karjagi, C. G., Lakshman, D. K., Hooda, K. S., & Kumar, A. (2018). Morphological and Molecular Variability among Indian Isolates of Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize. European Journal of Plant Pathology, 152(1), 45–60. https://doi.org/10.1007/s10658-018-1447-2
Stracquadanio, C., Quiles, J.M., Meca, G., & Cacciola, S.O. (2020). Antifungal Activity of Bioactive Metabolites Produced by Trichoderma asperellum and Trichoderma
atroviride in Liquid Medium. Journal of Fungi, 6(4), 263. https://doi.org/10.3390/jof6040263
Susheela, K., & Reddy, C.S. (2013). Variability in Rhizoctonia solani (AG1IA) Isolates Causing Sheath Blight of Rice in India. Indian Phytopathology, 66 (4), 341–350. Retrieved from https://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/36009/15962
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28(10), 2731–2739. https://doi.org/10.1093/molbev/msr121
Vinale, F., Ghisalberti, E., Sivasithamparam, K., Marra, R., Ritieni, A., Ferracane, R., ... Lorito, M. (2009). Factors Affecting the Production of Trichoderma harzianum Secondary Metabolites during the Interaction with Different Plant Pathogens. Letters in Applied Microbiology, 48(6), 705–711. https://doi.org/10.1111/j.1472-765x.2009.02599.x
Wang, L., Liu, L.M., Hou, Y.X., Li, L., & Huang, S.W. (2015). Pathotypic and Genetic Diversity in the Population of Rhizoctonia solani AG1-IA Causing Rice Sheath Blight in China. Plant Phytopathology, 64(3), 718–728. https://doi.org/10.1111/ppa.12299
White, T.J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In M.A. Innis, D.H. Gelfand, J.J. Sninsky, & J.J. White (Eds.), PCR Protocols: A Guide to Methods and Applications (pp. 315–322). San Diego, United States: Academic Press. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Wu, Q., Sun, R., Ni, M., Yu, J., Li, Y., Yu, C., ... Chen, J. (2017). Identification of a Novel Fungus, Trichoderma asperellum GDFS1009, and Comprehensive Evaluation of its Biocontrol Efficacy. PLoS ONE, 12(6), e0179957. https://doi.org/10.1371/journal.pone.0179957
Xian, H., Liu, L., Li, Y., Yang, Y., & Yang, S. (2019). Molecular Tagging of Biocontrol Fungus Trichoderma asperellum and its Colonization in Soil. Journal of Applied Microbiology, 128(1), 255–264. https://doi.org/10.1111/jam.14457
Yu, Z., Wang, Z., Zhang, Y., Wang, Y., & Liu, Z. (2021). Biocontrol and Growth-Promoting Effect of Trichoderma asperellum TaspHu1 Isolate from Juglans mandshurica Rhizosphere Soil. Microbiological Research, 242, 126596. https://doi.org/10.1016/j.micres.2020.126596
Zhang, C., Liu, Y., Ma, X., Feng, Z., & Ma, Z. (2009). Characterization of Sensitivity of Rhizoctonia solani, Causing Rice Sheath Blight, to Mepronil and Boscalid. Crop Protection, 28(5), 381–386. https://doi.org/10.1016/j.cropro.2008.12.004
Zhang, Y., & Zhuang, W.-Y. (2020). Trichoderma brevicrassum strain TC967 with Capacities of Diminishing Cucumber Disease Caused by Rhizoctonia solani and Promoting Plant Growth. Biological Control, 142, 104151. https://doi.org/10.1016/j.biocontrol.2019.104151
DOI: https://doi.org/10.22146/jpti.65290
Article Metrics
Abstract views : 3538
| 
views : 2011
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics