* Corresponding Author
The Resistance of Local Pigmented Rice Varieties against Bacterial Leaf Blight
https://doi.org/10.22146/jpti.46902 Tri Joko(1*), Kristamtini Kristamtini(2), Sumarno Sumarno(3), Rofiq Andriyanto(4)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Yogyakarta Assessment Institute for Agricultural Technology Jln. Stadion Maguwoharjo No. 22, Ngemplak, Sleman, Yogyakarta 55584
(3) Agriculture, Food and Fisheries Office of Sleman Regency Jln. Dr. Rajimin, Sucen, Triharjo, Sleman, Yogyakarta, 55511
(4) Agriculture, Food and Fisheries Office of Sleman Regency Jln. Dr. Rajimin, Sucen, Triharjo, Sleman, Yogyakarta, 55511
(*) Corresponding Author
Abstract
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important disease in rice plants worldwide, including in Indonesia. The use of resistant variety against BLB is the best effort in disease control because it is considered as the most effective and environmentally friendly. This study aimed to evaluate the resistance of local varieties, red rice (cv. Sembada Merah) and black rice (cv. Sembada Hitam), against Xoo compared to white rice variety (Ciherang), commonly cultivated by the farmers. The study was conducted in a greenhouse and arranged in Factorial of Completely Randomized Design (CRD) with 5 replications and 2 treatment factors: variety (Sembada Hitam, Sembada Merah, and Ciherang) and inoculation (with and without inoculated by Xoo bacterial suspension). Xoo inoculation was performed by clipping the leaves that close to the reproductive phase when the heading begins to be produced. The disease intensity and Area Under Disease Progress Curve (AUDPC) over two weeks were used to determine the level of resistance of the tested plant varieties. The results showed that black rice and red rice had better levels of resistance against BLB compared to white rice. At two weeks after inoculation, Sembada Hitam had the lowest AUDPC value followed by Sembada Merah, while the Ciherang had the highest AUDPC value and was categorized as a susceptible variety to Xoo.
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Adhikari, T.B., C.M.V. Cruz, Q. Zhang, R.J. Nelson, D.Z. Skinner, T.W. Mew, & J.E. Leach. 1995. Genetic Diversity of Xanthomonas oryzae pv. oryzae in Asia. Applied and Environmental Microbiology 61: 966–971.
Adhikari, T.B., R.C. Basnyat, & T.W. Mew. 1999. Virulence of Xanthomonas oryzae pv. oryzae on Rice Lines Containing Single Resistance Genes and Gene Combinations. Plant Disease 83: 46–50.
Akhtar, M.A., A. Rafi, & A. Hameed. 2008. Comparison of Methods of Inoculation of Xanthomonas oryzae pv. oryzae in Rice Cultivars. Pakistan Journal of Botany 40: 2171–2175.
Dardick, C., F.G. da Silva, Y. Shen, & P. Ronald. 2003. Antagonistic Interactions between Strains of Xanthomonas oryzae pv. oryzae. Phytopathology 93: 705–711.
Djatmiko, H.A., B. Prakoso, & N. Prihatiningsih. 2011. Penentuan Patotipe dan Keragaman Genetik Xanthomonas oryzae pv. oryzae pada Tanaman Padi di Wilayah Karesidenan Banyumas. Jurnal Hama dan Penyakit Tumbuhan Tropika 11: 35–46.
Gianinetti, A., F. Finocchiaro, F. Maisenti, D.K. Satsap, C. Morcia, R. Ghizzoni & V. Terzi. 2018. The Caryopsis of Red-Grained Rice Has Enhanced Resistance to Fungal Attack. Journal of Fungi 4: 1–12.
Handiyanti, M., T. Joko, & S. Subandiyah. 2018. Deteksi Molekuler Burkholderia glumae, Penyebab Penyakit Hawar Malai Padi. Jurnal Perlindungan Tanaman Indonesia 22: 98-107.
Herlina, L. & T.S. Silitonga. 2011. Seleksi Lapang Ketahanan Beberapa Varietas Padi terhadap Infeksi Hawar Daun Bakteri Strain IV dan VIII. Buletin Plasma Nutfah 17: 80–87.
Hidayah, E.N. 2017. Analisis Metabolomik Padi Hitam (Oryza sativa L.) setelah Infeksi Xanthomonas oryzae pv. oryzae. Tesis, Program Pascasarjana Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta. 101 p.
Ismiyatuningsih, T. Joko, & S. Hartono. 2016. Survey and Detection of Pectobacterium atrosepticum in Major Potato-Growing Areas in Central Java Province, Indonesia. Ilmu Pertanian 1: 1-6.
Joko, T., S. Subandiyah, & S. Somowiyarjo. 2000. The Role of Extracellular Protein on the Pathogenicity of Xanthomonas campestris pv. citri. Jurnal Perlindungan Tanaman Indonesia 6: 32-38.
Joko, T. 2017. Burkholderia glumae sebagai Emerging Pathogen: Status, Potensi kerusakan, dan Strategi Pengendalian. Pp. 27–35. Prosiding Simposium Nasional Fitopatologi 2017, Bogor.
Joko, T., M. Umehara, T. Murata, H. Etoh, K. Izumori, & S. Tsuyumu. 2018. Hyperinduction of Pectate Lyase in Dickeya chrysanthemi EC16 by Plant-Derived Sugars. Journal of Plant Interaction 13: 141-150.
Kangatharalingam, N., M.L. Pierce, M.B. Bayles, & M. Essenberg. 2002. Epidermal Anthocyanin Production as Indicator of Bacterial Blight Resistance in Cotton. Physiological and Molecular Plant Pathology 61: 189–195.
Kim, S.M., S. Jung-Pil, Q. Yang, N. Tae-Hwan, F.R. Russel, & K.J. Kshirod. 2015. Identification and Fine-Mapping of a New Resistance Gene, Xa40, Conferring Resistance to Bacterial Blight Races in Rice (Oryza sativa L.). Theoretical and Applied Genetics 128: 1933–1943.
Kristamtini & H. Purwaningsih. 2009. Potensi Pengembangan Beras Merah sebagai Plasma Nutfah Yogyakarta. Jurnal Litbang Pertanian 28: 88–95.
Kristamtini, Taryono, P. Basunanda, R.H. Murti, S. Supriyatna, Widyayanti & Sutarno. 2012. Morphological and Genetic Relations among Black Rice Landraces from Jogjakarta and Surounding Areas. ARPN Journal of Agriculturean and Biological Science 7: 982–987.
Mau, Y.S., A.S.S. Ndiwa, J.E.R. Markus, S.S. Oematan, A. Nasution, D.D. Handoko & K. Makbul. 2018. Blast Resistance Levels of Red and Black Upland Rice Local Cultivars from Indonesia. Asian Journal of Crop Science 10: 53–65.
Nino-Liu, D.O., P.C. Ronald, & A.J. Bogdanove. 2006. Xanthomonas oryzae Pathovars: Model Pathogens of a Model Crop. Molecular Plant Pathology 7: 303–324.
Nurjanah, N., T. Joko, & S. Subandiyah. 2017. Characterization of Pantoea ananatis Isolated from Garlic and Shallot. Jurnal Perlindungan Tanaman Indonesia 21: 120-126.
Sharma, P., L.C. Bora, K.C. Puzari, A.M. Baruah, R. Baruah, K. Talukdar, L. Kataky, & A. Phukan. 2017. Review on Bacterial Blight of Rice Caused by Xanthomonas oryzae pv. oryzae: Different Management Approaches and Role of Pseudomonas fluorescens As A Potential Biocontrol Agent. International Journal of Current Microbiology and Applied Science 6: 982–1005.
Solekha, R., F.A. Susanto, T. Joko, T.R. Nuringtyas, & Y.A. Purwestri. 2019. Phenylalanine Ammonia Lyase (PAL) Contributes to the Resistance of Black Rice against Xanthomonas oryzae pv. oryzae. Journal of Plant Pathology. https://doi.org/10.1007/s42161-019-00426-z.
Sudir, B. Nuryanto, & T.S. Kadir. 2012. Epidemiologi, Patotipe, dan Strategi Pengendalian Penyakit Hawar Daun Bakteri pada Tanaman Padi. Iptek Tanaman Pangan 7: 79–87.
Suparyono, Sudir, & Suprihanto. 2004. Pathotype Profile of Xanthomonas oryzae pv. oryzae Isolates from the Rice Ecosystem in Java. Indonesian Journal of Agricultural Science 5: 63–69.
Sutrisno, F.A. Susanto, P. Wijayanti, M.D. Retnoningrum, T.R. Nuringtyas, T. Joko, & Purwestri YA. 2018. Screening of Resistant Indonesian Black Rice Cultivars against Bacterial Leaf Blight. Euphytica 214: 199.
Suvendra, K.R., R. Rajeshwari, & R.V. Sonti. 2000. Mutants of Xanthomonas oryzae pv. oryzae Deficient in General Secretory Pathway are Virulence Deficient and Unable to Secrete Xylanase. Molecular Plant-Microbe Interactions 13: 394-401.
Trianom, B., T. Arwiyanto, & T. Joko. 2019. Morphological and molecular characterization of Sumatra disease of clove in Central Java, Indonesia. Tropical Life Sciences Research 30: 107–118.
Wahyudi, A.T., S. Meliah, & A.A. Nawangsih. 2011. Xanthomonas oryzae pv. oryzae Bakteri Penyebab Hawar Daun pada Padi: Isolasi, Karakterisasi, dan Telaah Mutagenesis dengan Transposon. Makara Sains 15: 89–96.
Wibowo A., T. Joko, S. Subandiyah, I. Mariska, Y. Supriyati, Y. Suryadi, & I. Roostika. 2010. Peningkatan Ketahanan Tanaman Pisang Kepok Kuning terhadap Penyakit Darah melalui Variasi Somaklonal dan Simbiosis Endofitik. Jurnal Perlindungan Tanaman Indonesia 16: 15–21.
Widyaningsih, S., S.N.H. Utami, T. Joko, & S. Subandiyah. 2017. Development of Disease and Growth on Six Scion/Root Stock Combinations of Citrus Seedlings under Huanglongbing Pressure. Journal of Agricultural Science 9: 229-238.
Widyaningsih, S., S.N.H. Utami, T. Joko, & S. Subandiyah. 2019. Plant Response and Huanglongbing Disease Development against Heat Treatments on ‘Siam Purworejo’ (Citrus nobilis (Lour)) and ‘Nambangan’ (C. maxima (Burm.) Merr.) under Field Condition. Archives of Phytopathology and Plant Protection 52: 259-276.
Xu, G.W., & C.F. Gonzalez. 1989. Evaluation of TN4431-induced Protease Mutants of Xanthomonas campestris pv. oryzae for Growth in Plants and Pathogenicity. Phytopathology 79: 1210-1215.
Yun, H.D., S.T. Lim, M.H. Chung, Y.W. Park, H.K. Kim, & K.Y. Kang. 1992. Genomic Cloning of the Extracellular Cellulase Gene from Xanthomonas oryzae pv. oryzae. Molecular Cells 2: 17-22.
Yun, H.D., Y.W. Park, S.T. Lim, H.K. Kim, & K.Y. Kang. 1993. The Role of the Extracellular Cellulase in Xanthomonas oryzae pv. oryzae on Bacterial Leaf Blight. Molecular Cells 3: 143-148.
Zhang, H. & S. Wang. 2013. Rice Versus Xanthomonas oryzae pv. oryzae: a Unique Pathosystem. Current Opinion in Plant Biology 16: 188–195.
DOI: https://doi.org/10.22146/jpti.46902
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