The Role of Nanochitosan on the Expression of Rice Resistance Genes against Bacterial Leaf Blight

https://doi.org/10.22146/jpti.44418

Siswanti Siswanti(1), Tri Joko(2), Siti Subandiyah(3*)

(1) Study Program of Biotechnology, The Graduate School of Universitas Gadjah Mada, Jln. Teknika Utara, Pogung, Mlati, Sleman, Yogyakarta, 55281
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has been reported to cause ± 20–50% of rice yield loss around the world. Resistant varieties are used to control this disease, however due to rapid evolution of this pathogen, the resistances was broken down in a few years. This study is aimed to determine the role of nanochitosan in the expression of rice Xa21 and Xa1 resistant genes against Xoo. The BLB susceptible rice cultivar IR64, the Xoo isolate MAG2 and a 0.065% concentration of nanochitosan were used in this experiment. Application of nanochitosan was carried out within 1-week intervals starting at rice aged 2–10 weeks after transplanting. The expression of Xa21 and Xa1 genes against Xoo were analyzed using conventional PCR and qPCR methods at 0 and 4 days after Xoo inoculation followed by 4x scoring of disease symptoms in 1-week interval. The treatments used in this study included the mock one/inoculated with sterile distilled water, K (+)/ plants inoculated with Xoo, CNP (-)/ with nanochitosan and sterile distilled water inoculation, and CNP (+)/ with nanochitosan and Xoo inoculation. The results showed that the 0.0065% concentration nanochitosan application was able to increase the expression of Xa21 and Xa1 genes on CNP (-). Disease intensity and AUDPC values did not show any significant difference between K (+) and CNP (+). This study concluded that nanochitosan at 0.065% was able to increase the expression of rice Xa21 and Xa1 resistance genes. However, the gene expression was not able to significantly suppress the infection development of Xoo.


Keywords


Bacterial Leaf Blight; nanochitosan; rice

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References

Adhikari, T.B., C.M.V. Cruz, Q. Zhang, R.J. Nelson, D.Z. Skinner, T.W. Mew, & J.E. Leach. 1995. Genetic Diversity of Xanthomonas oryzae pv. oryzae in Asia. Applied and Environmental Microbiology 61: 966-971.

Ahmed, H.U., M.R. Finckh, R.F. Alfonso, & C.C. Mundt. 1999. Epidemiological Effect of Gene Deployment Strategies on Bacterial Blight of Rice. Phytopathology 87: 66–70.

Akamatsu, A., K. Shimamoto, & Y. Kawano. 2016. Crosstalk of Signaling Mechanisms Involved in Host Defense and Symbiosis Against Microorganisms in Rice. Current Genomics 17: 297-307.

Century, K. S., R. A. Lagman, M. Adkisson, J. Morlan, R. Tobias, K. Schwartz, A. Smith, J. Love, P. C. Ronald, & M. C. Whalen. 1999. Developmental Control of Xa21-Mediated Disease Resistance in Rice. The Plant Journal 20: 231-236.

Hadrami, A. E., L. R. Adam, I. E. Hadrami, & F. Daayf. 2010. Chitosan in Plant Protection. Marine Drugs 8: 968-987.

Handani, W.R., W.B. Sediawan, A. Tawfiequrrahman, Wiratni, & Y. Kusumastuti. 2017. The Effect of Temperature and Chitosan Concentration during Storage on the Growth of Chitosan Nanoparticle Produced by Ionic Gelation Method. American Institute of Physics Conference Proceedings 1840: 080001.

IRRI (International Rice Research Institute). 2013. Standard Evaluation System (SES) for Rice. 5th edition. Manila, Philippines. 21 p.

Joko, T., Kristamtini, Sumarno, & R. Andriyanto. 2019. The Resistance of Local Pigmented Rice Varieties against Bacterial Leaf Blight. Jurnal Perlindungan Tanaman Indonesia 23: 205-210.

Joko, T., S. Subandiyah, & S. Somowiyarjo. 2000. The Role of Extracellular Protein on the Pathogenicity of Xanthomonas campestris pv. citri. Jurnal Perlindungan Tanaman Indonesia 6: 32-38.

Kobayashi, N.I. & K. Tanoi. 2015. Critical Issues in the Study of Magnesium Transport Systems and Magnesium Deficiency Symptoms in Plants. International Journal of Molecular Sciences 16: 23076-23093.

Livak, K. J. & T. D. Schmittgen. 2001. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-DDCT Method. Methods 25: 402-408.

Long, J., C. Song, F. Yan, J. Zhou, H. Zhou, & B. Yang. 2018. Non-TAL Effectors from Xanthomonas oryzae pv. oryzae Supress Peptidoglycan-Triggered MAPK Activation in Rice. Frontiers in Plant Science 9: 1857.

Manikandan, A. & M. Sathiyabama. 2015. Green Synthesis of Copper-chitosan Nanoparticles and Study of its Antibacterial Activity. Nanomedicine & Nanotechnology 6: 1–5.

Modina, I. M., L. Candelario, Calibo, & L. M. Borines. 2009. Antimicrobial Property of Chitosan and Induction of Systemic Acquired Resistance for the Control of Rice Bacterial Blight Caused by Xanthomonas oryzae pv. oryzae. Annals of Tropical Research 31: 69-89.

Park, C., S. Lee, M. Chern, R. Sharma, P. E. Canlas, M. Song, J. Jeon, & P. C. Ronald. 2011. Ectopic Expression of Rice Xa21 Overcomes Developmentally Controlled Resistance to Xanthomonas oryzae pv. oryzae. Plant Science 179: 466-471.

Park, C., Y. Peng, X. Chen, C. Dardick, D. Ruan, R. Bart, P. E. Canlas, & P. C. Ronald. 2008. Rice XB15, a Protein Phosphatase 2C, Negatively Regulates Cell Death and XA21-mediated Innate Immunity. PLoS Biology 6: e231.

Rusli, I. K., L. Soesanto, & R. F. Rahayuniati. 2016. Pengaruh Pupuk Organik Cair dan Asap Cair dalam Pengendalian Xanthomonas oryzae pv. oryzae dan Pyricularia grisea pada Padi Gogo Galur G136. Jurnal Perlindungan Tanaman Indonesia 20: 95-100.

Sasaki, T. & M. Ashikari. 2018. Rice Genomics, Genetics and Breeding. Springer Nature, Singapore, Pte, Ltd. 558 p.

Strange, R. N. 2003. Introduction to Plant Pathology. John Wiley and Sons Ltd., New York (US). 497 p.

Suryadi, Y., T.S. Kadir, & A.A. Daradjat. 2011. Evaluation of Disease Severity on Rice Genotypes to Bacterial Blight Using Amino Acid Content Analysis. Makara Journal of Science 15: 21-27.

Sutrisno, F. A. Susanto, P. Wijayanti, M. D. Retnoningrum, T. R. Nuringtyas, T. Joko, & Y. A. Purwestri. 2018. Screening of Resistant Indonesian Black Rice Cultivars against Bacterial Leaf Blight. Euphytica 214: 199.

Wahab, M.I, Satoto, R. Rachmat, A. Guswara, & Suharna. 2017. Deskripsi Varietas Unggul Baru Padi. Badan Penelitian dan Pengembangan Pertanian. Kementerian Pertanian, Subang. 19 p.

Wang, Y., L. Pi, X. Chen, P. K. Chakrabarty, J. Jiang, A. L. De Leon, G. Liu, L. Li, U. Benny, J. Oard, P. C. Ronald, & W. Song. 2006. Rice XA21 Binding Protein 3 is a Ubiquitin Ligase Required for Full Xa21-Mediated Disease Resistance. The Plant Cell 18: 3635-3646.

Wang, Y., Y. Xue, & J. Li. 2005. Towards Molecular Breeding and Improvement of Rice in China. TRENDS in Plant Science 10: 610.

Wang, W., N. Liu, C. Gao, L. Rui, & D. Tang. 2019. The Pseudomonas Syringae Effector AvrPtoB Associates with and Ubiquitinates Arabidopsis Exocyst Subunit EXO70B1. Frontiers in Plant Science 10: 1027.

Wheeder, B. E. 1969. An Introduction to Plant Diseases. John Wiley and Sons Limited, London. 301 p.

Xiang, T., N. Zong, Y. Zou, Y. Wu, J. Zhang, W. Xing, Y. Li, X. Tang, L. Zhu, J. Chai, & J-M. Zhou. 2008. Pseudomonas syringae Effector AvrPto Blocks Innate Immunity by Targeting Receptor Kinases. Current Biology 18: 74-80.

Yamaguchi, K., Y. Nakamura, K. Ishikawa, Y. Yoshimura, S. Tsuge, & T. Kawasaki. 2013. Supression of Rice Immunity by Xanthomonas oryzae type III Effector Xoo2875. Bioscience, Biotechnology, and Biochemistry 77: 796-801.

Yinggen, K., S. Hui, & M. Yuan. 2017. Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method. Bio-protocol 7: e2568.

Yoshimura, S., U. Yamanouchi, Y. Katayose, S. Toki, Z. Wang, I. Kono, N. Kurata, M. Yano, N. Iwata, & T. Sasaki. 1998. Expression of Xa1, a Bacterial Blight-Resistance Gene in Rice, is Induced by Bacterial Inoculation. Proceedings of the National Academy Science USA 95: 1663-1668.



DOI: https://doi.org/10.22146/jpti.44418

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